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- Publisher
- Springer Journals
- Copyright
- Copyright © 1990 by Nature Publishing Group
- Subject
- Science, Humanities and Social Sciences, multidisciplinary; Science, Humanities and Social Sciences, multidisciplinary; Science, multidisciplinary
- ISSN
- 0028-0836
- eISSN
- 1476-4687
- DOI
- 10.1038/347207a0
- Publisher site
- See Article on Publisher Site
Abstract
THE specificity of vesicular transport in a cell is determined by the formation of vesicles with specific contents from a donor compartment and their selective fusion with the appropriate acceptor compartment. Several of the latter fusion steps have been investigated in detail using cell-free systems1–9, and work with these systems as well as genetic evidence has revealed a role for GTP-binding proteins in membrane fusion processes7–15. We have reconstituted the formation of constitutive secretory vesicles and immature secretory granules from the trans Golgi network in a cell-free system16. We show here that the budding of both types of post-Golgi vesicles is inhibited by non-hydrolysable analogues of GTP, which suggests a more widespread role for GTP-binding proteins in membrane traffic than previously assumed.
Journal
Nature – Springer Journals
Published: Sep 13, 1990