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Myc and Ras collaborate in inducing accumulation of active cyclin E/Cdk2 and E2F

Myc and Ras collaborate in inducing accumulation of active cyclin E/Cdk2 and E2F Considerable evidence points to a role for Gl cyclin-dependent kinase (CDK) in allowing the accumulation of E2F transcription factor activity and induction of the S phase of the cell cycle1,2. Numerous experiments have also demonstrated a critical role for both Myc and Ras activities in allowing cell-cycle progression3. Here we show that inhibition of Ras activity blocks the normal growth-dependent activation of G1 CDK, prevents activation of the target genes of E2F, and results in cell-cycle arrest in G1. We also show that Ras is essential for entry into the S phase in Rb+/+fibroblasts but not in Rb-/- fibroblasts, establishing a link between Ras and the G1 CDK/Rb/E2F pathway. However, although expression of Ras alone will not induce G1 CDK activity or S phase, coexpression of Ras with Myc allows the generation of cyclin E-dependent kinase activity and the induction of S phase, coincident with the loss of the p27 cyclin-dependent kinase inhibitor (CKI). These results suggest that Ras, along with the activation of additional pathways, is required for the generation of G1 CDK activity, and that activation of cyclin E-dependent kinase in particular depends on the cooperative action of Ras and Myc. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nature Springer Journals

Myc and Ras collaborate in inducing accumulation of active cyclin E/Cdk2 and E2F

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References (29)

Publisher
Springer Journals
Copyright
Copyright © Nature Publishing Group 1997
Subject
Science, Humanities and Social Sciences, multidisciplinary; Science, Humanities and Social Sciences, multidisciplinary; Science, multidisciplinary
ISSN
0028-0836
eISSN
1476-4687
DOI
10.1038/387422a0
Publisher site
See Article on Publisher Site

Abstract

Considerable evidence points to a role for Gl cyclin-dependent kinase (CDK) in allowing the accumulation of E2F transcription factor activity and induction of the S phase of the cell cycle1,2. Numerous experiments have also demonstrated a critical role for both Myc and Ras activities in allowing cell-cycle progression3. Here we show that inhibition of Ras activity blocks the normal growth-dependent activation of G1 CDK, prevents activation of the target genes of E2F, and results in cell-cycle arrest in G1. We also show that Ras is essential for entry into the S phase in Rb+/+fibroblasts but not in Rb-/- fibroblasts, establishing a link between Ras and the G1 CDK/Rb/E2F pathway. However, although expression of Ras alone will not induce G1 CDK activity or S phase, coexpression of Ras with Myc allows the generation of cyclin E-dependent kinase activity and the induction of S phase, coincident with the loss of the p27 cyclin-dependent kinase inhibitor (CKI). These results suggest that Ras, along with the activation of additional pathways, is required for the generation of G1 CDK activity, and that activation of cyclin E-dependent kinase in particular depends on the cooperative action of Ras and Myc.

Journal

Nature – Springer Journals

Published: May 22, 1997

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