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Toxicological Sciences, kfab145, https://doi.org/10.1093/toxsci/kfab145 In the originally published version of this manuscript, the images of Figs 8-12 were erroneously transposed and consequently incorrect for the legends beneath. Figure 9 published as Fig. 8; Fig. 10 as Fig. 9; Fig.11 as Fig. 10; Fig. 12 as Fig. 11; and Fig. 8 as Fig. 12. Figure 8 should appear as Instead ofFigure 9 should appear as:Instead of.Figure 10 should appear as:Instead ofFigure 11 should appear as: Instead ofFigure 12 should appear as:Instead of. These errors have now been corrected online. Figure 8. Open in new tabDownload slide Comparisons of the fold changes in mRNA expression levels for selected bisphenol S (BPS) target genes determined by RNA sequencing (left panels) with those measured by quantitative reverse transcription-polymerase chain reaction (qRT-PCR; right panels). Limbs were exposed to vehicle (blue) or 50 μM BPS (purple) for 24 h (A and B) or 48 h (C and D). qRT-PCR expression was normalized to Rpl8. Two-way ANOVA with Bonferroni’s multiple comparisons test was performed; ‡p < .01 control versus 50 μM BPS (n = 5). Figure 8. Open in new tabDownload slide Comparisons of the fold changes in mRNA expression levels for selected bisphenol S (BPS) target genes determined by RNA sequencing (left panels) with those measured by quantitative reverse transcription-polymerase chain reaction (qRT-PCR; right panels). Limbs were exposed to vehicle (blue) or 50 μM BPS (purple) for 24 h (A and B) or 48 h (C and D). qRT-PCR expression was normalized to Rpl8. Two-way ANOVA with Bonferroni’s multiple comparisons test was performed; ‡p < .01 control versus 50 μM BPS (n = 5). Figure 9. Open in new tabDownload slide Comparisons of the fold changes in mRNA expression levels for 2 retinoic acid-related genes determined by RNA sequencing (left panels) with those measured by quantitative reverse transcription-polymerase chain reaction (qRT-PCR; right panels). Limbs were exposed to vehicle (blue), 10 μM bisphenol A (BPA; green), or 50 μM BPA (purple) for 3 h (A and B), 24 h (C and D), or 48 h (E and F). qRT-PCR expression was normalized to Rpl8. Two-way ANOVA with Dunnett’s test was performed; †p < .01 control versus 10 μM BPA, ‡p < .01 control versus 50 μM BPA (n = 5). Figure 9. Open in new tabDownload slide Comparisons of the fold changes in mRNA expression levels for 2 retinoic acid-related genes determined by RNA sequencing (left panels) with those measured by quantitative reverse transcription-polymerase chain reaction (qRT-PCR; right panels). Limbs were exposed to vehicle (blue), 10 μM bisphenol A (BPA; green), or 50 μM BPA (purple) for 3 h (A and B), 24 h (C and D), or 48 h (E and F). qRT-PCR expression was normalized to Rpl8. Two-way ANOVA with Dunnett’s test was performed; †p < .01 control versus 10 μM BPA, ‡p < .01 control versus 50 μM BPA (n = 5). Figure 10. Open in new tabDownload slide Comparisons of the fold changes in mRNA expression levels for 4 RhoGDI signaling genes determined by RNA sequencing (left panels) with those measured by quantitative reverse transcription-polymerase chain reaction (qRT-PCR; right panels). Limbs were exposed to vehicle (blue), 10 μM bisphenol A (BPA; green), or 50 μM BPA (purple) for 3 h (A and B), 24 h (C and D), or 48 h (E and F). qRT-PCR expression was normalized to Rpl8. Two-way ANOVA with Dunnett’s test was performed; †p < .01 control versus 10 μM BPA, ‡p < .01 control versus 50 μM BPA (n = 5). Figure 10. Open in new tabDownload slide Comparisons of the fold changes in mRNA expression levels for 4 RhoGDI signaling genes determined by RNA sequencing (left panels) with those measured by quantitative reverse transcription-polymerase chain reaction (qRT-PCR; right panels). Limbs were exposed to vehicle (blue), 10 μM bisphenol A (BPA; green), or 50 μM BPA (purple) for 3 h (A and B), 24 h (C and D), or 48 h (E and F). qRT-PCR expression was normalized to Rpl8. Two-way ANOVA with Dunnett’s test was performed; †p < .01 control versus 10 μM BPA, ‡p < .01 control versus 50 μM BPA (n = 5). Figure 11. Open in new tabDownload slide Comparisons of the fold changes in mRNA expression levels for 7 p53 signaling pathway genes determined by RNA sequencing (left panels) with those measured by quantitative reverse transcription-polymerase chain reaction (qRT-PCR; right panels). Limbs were exposed to vehicle (blue), 1 μM BPAF (green), or 5 μM bisphenol AF (BPAF; purple) for 3 h (A and B), 24 h (C and D), or 48 h (E and F). quantitative RT-PCR expression was normalized to Rpl8. Two-way ANOVA with Dunnett’s test was performed; †p < .01 control versus 1 μM BPAF, ‡p < .01 control versus 5 μM BPAF (n = 5). Figure 11. Open in new tabDownload slide Comparisons of the fold changes in mRNA expression levels for 7 p53 signaling pathway genes determined by RNA sequencing (left panels) with those measured by quantitative reverse transcription-polymerase chain reaction (qRT-PCR; right panels). Limbs were exposed to vehicle (blue), 1 μM BPAF (green), or 5 μM bisphenol AF (BPAF; purple) for 3 h (A and B), 24 h (C and D), or 48 h (E and F). quantitative RT-PCR expression was normalized to Rpl8. Two-way ANOVA with Dunnett’s test was performed; †p < .01 control versus 1 μM BPAF, ‡p < .01 control versus 5 μM BPAF (n = 5). Figure 12. Open in new tabDownload slide Comparisons of the fold changes in mRNA expression levels for selected bisphenol AF (BPAF) target genes determined by RNA sequencing (left panels) with those measured by quantitative reverse transcription-polymerase chain reaction (qRT-PCR; right panels). Limbs were exposed to vehicle (blue), 1 μM BPAF (green), or 5 μM BPAF (purple) for 3 h (A and B), 24 h (C and D), or 48 h (E and F). qRT-PCR expression was normalized to Rpl8. Two-way ANOVA with Dunnett’s test was performed; †p < .01 control versus 1 μM BPAF, ‡p < .01 control versus 5 μM BPAF (n = 5). Figure 12. Open in new tabDownload slide Comparisons of the fold changes in mRNA expression levels for selected bisphenol AF (BPAF) target genes determined by RNA sequencing (left panels) with those measured by quantitative reverse transcription-polymerase chain reaction (qRT-PCR; right panels). Limbs were exposed to vehicle (blue), 1 μM BPAF (green), or 5 μM BPAF (purple) for 3 h (A and B), 24 h (C and D), or 48 h (E and F). qRT-PCR expression was normalized to Rpl8. Two-way ANOVA with Dunnett’s test was performed; †p < .01 control versus 1 μM BPAF, ‡p < .01 control versus 5 μM BPAF (n = 5). © The Author(s) 2022. Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved. For permissions, please e-mail: [email protected] This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)
Toxicological Sciences – Oxford University Press
Published: Feb 12, 2022
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