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Correlation of apparent diffusion coefficient values measured by diffusion MRI and MGMT promoter methylation semiquantitatively analyzed with MS‐MLPA in patients with glioblastoma multiforme

Correlation of apparent diffusion coefficient values measured by diffusion MRI and MGMT promoter... Purpose: To retrospectively determine whether the apparent diffusion coefficient (ADC) values correlate with O6‐methylguanine DNA methyltransferase (MGMT) promoter methylation semiquantitatively analyzed by methylation‐specific multiplex ligation‐dependent probe amplification (MS‐MLPA) in patients with glioblastoma. Materials and Methods: The study was approved by the Institutional Review Board and was Health Insurance Portability and Accountability Act (HIPAA) compliant. Newly diagnosed patients with glioblastoma (n = 26) were analyzed with an ADC histogram approach based on enhancing solid portion. The methylation status of MGMT promoter was assessed by methylation‐specific polymerase chain reaction (MSP) and by MS‐MLPA. MS‐MLPA is a semiquantitative method that determines the methylation ratio. The Ki‐67 labeling index was also analyzed. The mean and 5th percentile ADC values were correlated with MGMT promoter methylation status and Ki‐67 labeling index using a linear regression model. Progression‐free survival (PFS) was also correlated with the ADC values using Kaplan–Meier survival analysis. Results: The mean methylation ratio was 0.21 ± 0.20. By MSP, there were 5 methylated and 21 unmethylated tumors. The mean ADC revealed a positive relationship with MGMT promoter methylation ratio (P = 0.015) and was also significantly different according to MSP‐determined methylation status (P = 0.011). Median PFS was significantly related with methylation ratio (P = 0.017) and MSP‐derived methylation status (P = 0.025). A positive relationship was demonstrated between PFS and the mean ADC value (P = 0.001). The 5th percentile ADC values showed a significant negative relationship with Ki‐67 labeling index (P = 0.036). Conclusion: We found that ADC values were significantly correlated with PFS as well as with MGMT promoter methylation status. We believe that ADC values may merit further investigation as a noninvasive biomarker for predicting treatment response. J. Magn. Reson. Imaging 2013;37:351–358. © 2012 Wiley Periodicals, Inc. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Magnetic Resonance Imaging Wiley

Correlation of apparent diffusion coefficient values measured by diffusion MRI and MGMT promoter methylation semiquantitatively analyzed with MS‐MLPA in patients with glioblastoma multiforme

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References (33)

Publisher
Wiley
Copyright
Copyright © 2012 Wiley Periodicals, Inc.
ISSN
1053-1807
eISSN
1522-2586
DOI
10.1002/jmri.23838
pmid
23023975
Publisher site
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Abstract

Purpose: To retrospectively determine whether the apparent diffusion coefficient (ADC) values correlate with O6‐methylguanine DNA methyltransferase (MGMT) promoter methylation semiquantitatively analyzed by methylation‐specific multiplex ligation‐dependent probe amplification (MS‐MLPA) in patients with glioblastoma. Materials and Methods: The study was approved by the Institutional Review Board and was Health Insurance Portability and Accountability Act (HIPAA) compliant. Newly diagnosed patients with glioblastoma (n = 26) were analyzed with an ADC histogram approach based on enhancing solid portion. The methylation status of MGMT promoter was assessed by methylation‐specific polymerase chain reaction (MSP) and by MS‐MLPA. MS‐MLPA is a semiquantitative method that determines the methylation ratio. The Ki‐67 labeling index was also analyzed. The mean and 5th percentile ADC values were correlated with MGMT promoter methylation status and Ki‐67 labeling index using a linear regression model. Progression‐free survival (PFS) was also correlated with the ADC values using Kaplan–Meier survival analysis. Results: The mean methylation ratio was 0.21 ± 0.20. By MSP, there were 5 methylated and 21 unmethylated tumors. The mean ADC revealed a positive relationship with MGMT promoter methylation ratio (P = 0.015) and was also significantly different according to MSP‐determined methylation status (P = 0.011). Median PFS was significantly related with methylation ratio (P = 0.017) and MSP‐derived methylation status (P = 0.025). A positive relationship was demonstrated between PFS and the mean ADC value (P = 0.001). The 5th percentile ADC values showed a significant negative relationship with Ki‐67 labeling index (P = 0.036). Conclusion: We found that ADC values were significantly correlated with PFS as well as with MGMT promoter methylation status. We believe that ADC values may merit further investigation as a noninvasive biomarker for predicting treatment response. J. Magn. Reson. Imaging 2013;37:351–358. © 2012 Wiley Periodicals, Inc.

Journal

Journal of Magnetic Resonance Imaging – Wiley

Published: Feb 1, 2013

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