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THE EFFECTS OF OXYGEN DEPRIVATION ON ELECTRICALLY STIMULATED CEREBRAL CORTEX SLICES

THE EFFECTS OF OXYGEN DEPRIVATION ON ELECTRICALLY STIMULATED CEREBRAL CORTEX SLICES Abstract— (1) Thin slices were prepared from guinea pig cerebral cortex and allowed to incubate in oxygenated bicarbonate‐buffered medium for 30 min. Subsequent to that time the slices were made hypoxic by passing 95% N2‐5% CO2 through the medium. Hypoxic exposure caused the slices to gain Na+ and to lose K+ ions from the non‐inulin space. These shifts were especially pronounced when slices were electrically stimulated during the hypoxic period. Thus, after 30 min of hypoxia plus stimulation, non‐inulin Na+ had risen from 30 to 84, μequiv./g wet wt., and non‐inulin K+ had fallen from 50·5 to 14·3 μequiv./g wet wt. (2) The above shifts were in part reversible, but when reoxygenated slices were subsequently electrically stimulated in oxygenated media, they failed to lose K+ or to gain Na+. (3) The induced inexcitable state could not be attributed to inability of the slices to replenish ATP and phosphocreatine and may indicate an alteration in membrane constituents necessary for preservation of membrane excitability. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neurochemistry Wiley

THE EFFECTS OF OXYGEN DEPRIVATION ON ELECTRICALLY STIMULATED CEREBRAL CORTEX SLICES

SWANSON, P. D.
Journal of Neurochemistry , Volume 16 (1) – Jan 1, 1969
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References (23)

Publisher
Wiley
Copyright
Copyright © 1969 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0022-3042
eISSN
1471-4159
DOI
10.1111/j.1471-4159.1969.tb10341.x
Publisher site
See Article on Publisher Site

Abstract

Abstract— (1) Thin slices were prepared from guinea pig cerebral cortex and allowed to incubate in oxygenated bicarbonate‐buffered medium for 30 min. Subsequent to that time the slices were made hypoxic by passing 95% N2‐5% CO2 through the medium. Hypoxic exposure caused the slices to gain Na+ and to lose K+ ions from the non‐inulin space. These shifts were especially pronounced when slices were electrically stimulated during the hypoxic period. Thus, after 30 min of hypoxia plus stimulation, non‐inulin Na+ had risen from 30 to 84, μequiv./g wet wt., and non‐inulin K+ had fallen from 50·5 to 14·3 μequiv./g wet wt. (2) The above shifts were in part reversible, but when reoxygenated slices were subsequently electrically stimulated in oxygenated media, they failed to lose K+ or to gain Na+. (3) The induced inexcitable state could not be attributed to inability of the slices to replenish ATP and phosphocreatine and may indicate an alteration in membrane constituents necessary for preservation of membrane excitability.

Journal

Journal of Neurochemistry – Wiley

Published: Jan 1, 1969

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