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Telomerase- and recombination-independent immortalization of budding yeast

Telomerase- and recombination-independent immortalization of budding yeast Downloaded from genesdev.cshlp.org on December 16, 2021 - Published by Cold Spring Harbor Laboratory Press Telomerase- and recombination- independent immortalization of budding yeast Laura Maringele and David Lydall University of Newcastle, School of Clinical Medical Sciences-Gerontology, Newcastle upon Tyne, NE4 6BE, United Kingdom It is generally assumed that there are only two ways to maintain the ends of chromosomes in yeast and mammalian nuclei: telomerase and recombination. Without telomerase and recombination, cells enter senescence, a state of permanent growth arrest. We found that the decisive role in preventing senescent budding yeast cells from dividing is played by the Exo1 nuclease. In the absence of Exo1, telomerase- and recombination-defective yeast can resume cell cycle progression, despite degradation of telomeric regions from many chromosomes. As degradation progresses toward internal chromosomal regions, a progressive decrease in viability would be expected, caused by loss of essential genes. However, this was not the case. We demonstrate that extensive degradation and loss of essential genes can be efficiently prevented through a little-studied mechanism of DNA double-strand-break repair, in which short DNA palindromes induce formation of large DNA palindromes. For the first time, we show that large palindromes form as a natural consequence of postsenescence growth http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Genes & Development Unpaywall

Telomerase- and recombination-independent immortalization of budding yeast

Maringele, Laura; Lydall, David
Genes & Development – Oct 15, 2004
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Unpaywall
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0890-9369
DOI
10.1101/gad.316504
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Abstract

Downloaded from genesdev.cshlp.org on December 16, 2021 - Published by Cold Spring Harbor Laboratory Press Telomerase- and recombination- independent immortalization of budding yeast Laura Maringele and David Lydall University of Newcastle, School of Clinical Medical Sciences-Gerontology, Newcastle upon Tyne, NE4 6BE, United Kingdom It is generally assumed that there are only two ways to maintain the ends of chromosomes in yeast and mammalian nuclei: telomerase and recombination. Without telomerase and recombination, cells enter senescence, a state of permanent growth arrest. We found that the decisive role in preventing senescent budding yeast cells from dividing is played by the Exo1 nuclease. In the absence of Exo1, telomerase- and recombination-defective yeast can resume cell cycle progression, despite degradation of telomeric regions from many chromosomes. As degradation progresses toward internal chromosomal regions, a progressive decrease in viability would be expected, caused by loss of essential genes. However, this was not the case. We demonstrate that extensive degradation and loss of essential genes can be efficiently prevented through a little-studied mechanism of DNA double-strand-break repair, in which short DNA palindromes induce formation of large DNA palindromes. For the first time, we show that large palindromes form as a natural consequence of postsenescence growth

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Genes & Development – Unpaywall

Published: Oct 15, 2004

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