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Activity of Genital Tract Secretions and Synthetic Antimicrobial Peptides against Group B Streptococcus

Activity of Genital Tract Secretions and Synthetic Antimicrobial Peptides against Group B... Problem Genital tract secretions inhibit Escherichia coli (E. coli) through antimicrobial peptides (AMP) secreted by the host and vaginal microbiota. However, there are limited data against group B Streptococcus (GBS). Method of Study Group B Streptococcus were incubated with cervico‐vaginal lavage (CVL) samples from healthy non‐pregnant women (n = 12) or synthetic AMP and monitored for bacterial growth using a turbidimetric approach. E. coli inhibitory activity was determined by a colony‐forming unit assay. Results None of the CVL samples inhibited GBS. The human neutrophil peptide‐1 and human defensin 5 inhibited GBS growth by ≥80% at concentrations ≥20 μg/mL and ≥50 μg/mL, respectively, while human beta‐defensin 2 and LL‐37 did not inhibit at highest concentration tested (100 μg/mL). In contrast, all AMP inhibited E. coli. Conclusions Antimicrobial peptides may protect against E. coli colonization but have more limited activity against GBS. Future studies will focus on augmenting host defense with specific AMP to prevent genitourinary infection with these pathogenic organisms. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png American Journal of Reproductive Immunology Wiley

Activity of Genital Tract Secretions and Synthetic Antimicrobial Peptides against Group B Streptococcus

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References (48)

Publisher
Wiley
Copyright
Copyright © 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
ISSN
1046-7408
eISSN
1600-0897
DOI
10.1111/aji.12427
pmid
26395128
Publisher site
See Article on Publisher Site

Abstract

Problem Genital tract secretions inhibit Escherichia coli (E. coli) through antimicrobial peptides (AMP) secreted by the host and vaginal microbiota. However, there are limited data against group B Streptococcus (GBS). Method of Study Group B Streptococcus were incubated with cervico‐vaginal lavage (CVL) samples from healthy non‐pregnant women (n = 12) or synthetic AMP and monitored for bacterial growth using a turbidimetric approach. E. coli inhibitory activity was determined by a colony‐forming unit assay. Results None of the CVL samples inhibited GBS. The human neutrophil peptide‐1 and human defensin 5 inhibited GBS growth by ≥80% at concentrations ≥20 μg/mL and ≥50 μg/mL, respectively, while human beta‐defensin 2 and LL‐37 did not inhibit at highest concentration tested (100 μg/mL). In contrast, all AMP inhibited E. coli. Conclusions Antimicrobial peptides may protect against E. coli colonization but have more limited activity against GBS. Future studies will focus on augmenting host defense with specific AMP to prevent genitourinary infection with these pathogenic organisms.

Journal

American Journal of Reproductive Immunology – Wiley

Published: Dec 1, 2015

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