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References (39)
-
CHRISTOPHER Smith, Dana Smith, J. Derisi, E. Blackburn (2003)
Telomeric protein distributions and remodeling through the cell cycle in Saccharomyces cerevisiae.Molecular biology of the cell, 14 2
-
J. Lin (1996)
The Saccharomyces CDC13 is a single-strand TG1-3 telomeric DNA-binding protein in vitro that affects telomere in vivo, 93
-
K. Huffman, S. Levene, V. Tesmer, J. Shay, W. Wright (2000)
Telomere Shortening Is Proportional to the Size of the G-rich Telomeric 3′-Overhang*The Journal of Biological Chemistry, 275
-
A. Taggart, S. Teng, V. Zakian (2002)
Est1p As a Cell Cycle-Regulated Activator of Telomere-Bound TelomeraseScience, 297
-
S. Brill, B. Stillman (1991)
Replication factor-A from Saccharomyces cerevisiae is encoded by three essential genes coordinately expressed at S phase.Genes & development, 5 9
-
H. Maniar, R. Wilson, S. Brill (1997)
Roles of replication protein-A subunits 2 and 3 in DNA replication fork movement in Saccharomyces cerevisiae.Genetics, 145 4
-
M. Longhese, P. Plevani, G. Lucchini (1994)
Replication factor A is required in vivo for DNA replication, repair, and recombinationMolecular and Cellular Biology, 14
-
C. Price (1997)
Synthesis of the telomeric C-strand. A review.Biochemistry. Biokhimiia, 62 11
-
B. Bourns, M. Alexander, Andrew Smith, V. Zakian (1998)
Sir Proteins, Rif Proteins, and Cdc13p BindSaccharomyces Telomeres In VivoMolecular and Cellular Biology, 18
-
S. Marcand, Vanessa Brevet, E. Gilson (1999)
Progressive cis‐inhibition of telomerase upon telomere elongationThe EMBO Journal, 18
-
G. Brush, Dawn Clifford, Suzanne Marinco, Amy Bartrand (2001)
Replication protein A is sequentially phosphorylated during meiosis.Nucleic acids research, 29 23
-
Y. Tsukamoto, A. Taggart, V. Zakian (2001)
The role of the Mre11-Rad50-Xrs2 complex in telomerase- mediated lengthening of Saccharomyces cerevisiae telomeresCurrent Biology, 11
-
Scott Diede, D. Gottschling (1999)
Telomerase-Mediated Telomere Addition In Vivo Requires DNA Primase and DNA Polymerases α and δCell, 99
-
E. Pennock, K. Buckley, V. Lundblad (2001)
Cdc13 Delivers Separate Complexes to the Telomere for End Protection and ReplicationCell, 104
-
S. Evans, V. Lundblad (1999)
Est1 and Cdc13 as comediators of telomerase access.Science, 286 5437
-
R. Polotnianka, Jian Li, A. Lustig (1998)
The yeast Ku heterodimer is essential for protection of the telomere against nucleolytic and recombinational activitiesCurrent Biology, 8
-
P. Greenwell, Shara Kronmal, S. Porter, Johann Gassenhuber, B. Obermaier, T. Petes (1995)
TEL1, a gene involved in controlling telomere length in S. cerevisiae, is homologous to the human ataxia telangiectasia geneCell, 82
-
S. Gravel, Michel Larrivée, P. Labrecque, R. Wellinger (1998)
Yeast Ku as a regulator of chromosomal DNA end structure.Science, 280 5364
-
Jing-Jer Lin, V. Zakian (1996)
The Saccharomyces CDC13 protein is a single-strand TG1-3 telomeric DNA-binding protein in vitro that affects telomere behavior in vivo.Proceedings of the National Academy of Sciences of the United States of America, 93 24
-
R. Craven, T. Petes (1999)
Dependence of the regulation of telomere length on the type of subtelomeric repeat in the yeast Saccharomyces cerevisiae.Genetics, 152 4
-
I. Dornreiter, L. Erdile, U. Gilbert, Dorothea Winkler, T. Kelly, E. Fanning (1992)
Interaction of DNA polymerase alpha‐primase with cellular replication protein A and SV40 T antigen.The EMBO Journal, 11
-
Vera Schramke, H. Neecke, Vanessa Brevet, Y. Corda, Giovanna Lucchini, M. Longhese, Eric Gilson, Vincent Géli (2001)
The set1Delta mutation unveils a novel signaling pathway relayed by the Rad53-dependent hyperphosphorylation of replication protein A that leads to transcriptional activation of repair genes.Genes & development, 15 14
-
S. Diede, D. Gottschling (1999)
Telomerase-mediated telomere addition in vivo requires DNA primase and DNA polymerases alpha and delta.Cell, 99 7
-
B. Snow, N. Erdmann, Jennifer Cruickshank, Hartt Goldman, R. Gill, M. Robinson, L. Harrington (2003)
Functional Conservation of the Telomerase Protein Est1p in HumansCurrent Biology, 13
-
G. Brush, D. Morrow, P. Hieter, T. Kelly (1996)
The ATM homologue MEC1 is required for phosphorylation of replication protein A in yeast.Proceedings of the National Academy of Sciences of the United States of America, 93 26
-
Tomoyuki Tanaka, K. Nasmyth (1998)
Association of RPA with chromosomal replication origins requires an Mcm protein, and is regulated by Rad53, and cyclin‐ and Dbf4‐dependent kinasesThe EMBO Journal, 17
-
M. Longhese, H. Neecke, V. Paciotti, G. Lucchini, P. Plevani (1996)
The 70 kDa subunit of replication protein A is required for the G1/S and intra-S DNA damage checkpoints in budding yeast.Nucleic acids research, 24 18
-
M. Wold (1997)
Replication protein A: a heterotrimeric, single-stranded DNA-binding protein required for eukaryotic DNA metabolism.Annual review of biochemistry, 66
-
S. Chan, Jennifer Chang, J. Prescott, E. Blackburn (2001)
Altering telomere structure allows telomerase to act in yeast lacking ATM kinasesCurrent Biology, 11
-
P. Reichenbach, M. Höss, C. Azzalin, M. Nabholz, P. Bucher, J. Lingner (2003)
A Human Homolog of Yeast Est1 Associates with Telomerase and Uncaps Chromosome Ends When OverexpressedCurrent Biology, 13
-
R. Wellinger, A. Wolf, V. Zakian (1993)
Saccharomyces telomeres acquire single-strand TG1–3 tails late in S phaseCell, 72
-
S. Peterson, A. Stellwagen, S. Diede, Miriam Singer, Zara Haimberger, Catherine Johnson, M. Tzoneva, D. Gottschling (2001)
The function of a stem-loop in telomerase RNA is linked to the DNA repair protein KuNature Genetics, 27
-
C. Nugent, V. Lundblad (1998)
The telomerase reverse transcriptase: components and regulation.Genes & development, 12 8
-
Sophie Martin, T. Laroche, N. Suka, M. Grunstein, S. Gasser (1999)
Relocalization of Telomeric Ku and SIR Proteins in Response to DNA Strand Breaks in YeastCell, 97
-
M. McEachern, A. Krauskopf, E. Blackburn (2000)
Telomeres and their control.Annual review of genetics, 34
-
G. Brush, T. Kelly (2000)
Phosphorylation of the replication protein A large subunit in the Saccharomyces cerevisiae checkpoint response.Nucleic acids research, 28 19
-
K. Ritchie, T. Petes (2000)
The Mre11p/Rad50p/Xrs2p complex and the Tel1p function in a single pathway for telomere maintenance in yeast.Genetics, 155 1
-
J. Mallory, V. Bashkirov, Kelly Trujillo, J. Solinger, M. Dominska, P. Sung, W. Heyer, T. Petes (2003)
Amino acid changes in Xrs2p, Dun1p, and Rfa2p that remove the preferred targets of the ATM family of protein kinases do not affect DNA repair or telomere length in Saccharomyces cerevisiae.DNA repair, 2 9
-
Julianne Smith, Hui Zou, R. Rothstein (2000)
Characterization of genetic interactions with RFA1: the role of RPA in DNA replication and telomere maintenance.Biochimie, 82 1
- Publisher
- Springer Journals
- Copyright
- Copyright © 2004 by Nature Publishing Group
- Subject
- Biomedicine; Biomedicine, general; Human Genetics; Cancer Research; Agriculture; Gene Function; Animal Genetics and Genomics
- ISSN
- 1061-4036
- eISSN
- 1546-1718
- DOI
- 10.1038/ng1284
- Publisher site
- See Article on Publisher Site
Abstract
Replication protein A (RPA) is a highly conserved single-stranded DNA–binding protein involved in DNA replication, recombination and repair. We show here that RPA is present at the telomeres of the budding yeast Saccharomyces cerevisiae, with a maximal association in S phase. A truncation of the N-terminal region of Rfa2p (associated with the rfa2Δ40 mutated allele) results in severe telomere shortening caused by a defect in the in vivo regulation of telomerase activity. Cells carrying rfa2Δ40 show impaired binding of the protein Est1p, which is required for telomerase action. In addition, normal telomere length can be restored by expressing a Cdc13-Est1p hybrid protein. These findings indicate that RPA activates telomerase by loading Est1p onto telomeres during S phase. We propose a model of in vivo telomerase action that involves synergistic action of RPA and Cdc13p at the G-rich 3′ overhang of telomeric DNA.
Journal
Nature Genetics – Springer Journals
Published: Dec 21, 2003