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Cytochemistry of undamaged neurons transporting exogenous protein in vivo

Cytochemistry of undamaged neurons transporting exogenous protein in vivo The uptake, transport and fate of exogenous protein in undamaged cranial motor and neurosecretory neurons in mice have been investigated using enzyme cytochemical techniques for horseradish peroxidase (HRP) and lysosomal acid hydrolases. Labeling of these neuronal perikarya with HRP is accomplished by retrograde axoplasmic transport from their axon terminals following vascular injection of the protein or by direct somal‐dendritic uptake subsequent to cerebral ventriculo‐cisternal perfusion of peroxidase. Axon terminals of neurosecretory neurons innervating the posterior pituitary gland and those of cranial motoneurons can remain unexposed to HRP administered intraventricularly and, therefore, cannot directly incorporate the protein. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Comparative Neurology Wiley

Cytochemistry of undamaged neurons transporting exogenous protein in vivo

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References (96)

Publisher
Wiley
Copyright
"Copyright © 1979 Wiley Subscription Services, Inc., A Wiley Company"
ISSN
0021-9967
eISSN
1096-9861
DOI
10.1002/cne.901850104
pmid
85639
Publisher site
See Article on Publisher Site

Abstract

The uptake, transport and fate of exogenous protein in undamaged cranial motor and neurosecretory neurons in mice have been investigated using enzyme cytochemical techniques for horseradish peroxidase (HRP) and lysosomal acid hydrolases. Labeling of these neuronal perikarya with HRP is accomplished by retrograde axoplasmic transport from their axon terminals following vascular injection of the protein or by direct somal‐dendritic uptake subsequent to cerebral ventriculo‐cisternal perfusion of peroxidase. Axon terminals of neurosecretory neurons innervating the posterior pituitary gland and those of cranial motoneurons can remain unexposed to HRP administered intraventricularly and, therefore, cannot directly incorporate the protein.

Journal

The Journal of Comparative NeurologyWiley

Published: Jan 1, 1979

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