Access the full text.
Sign up today, get DeepDyve free for 14 days.
Loading next page...
/lp/oxford-university-press/enzymatic-determination-of-total-serum-cholesterol-RMyJWlkLWb
References (20)
-
S. Brown (1973)
Notes on the Quality of Performance of Serum Cholesterol AssaysAnnals of Clinical Biochemistry, 10
-
C. Fasce, R. Vanderlinde (1972)
Factors affecting the results of serum cholesterol determinations: an interlaboratory evaluation.Clinical chemistry, 18 9
-
H. Hernandez, I. Chaikoff (1957)
Purification and properties of pancreatic cholesterol esterase.The Journal of biological chemistry, 228 1
-
R. Conn (1964)
Clinical Chemistry: Principles and TechnicsJAMA, 189
-
(1972)
Lab. Invest -
(1961)
Fig. 12. Correlation of results by the method of Abell and present method -
N. Gochman, J. Schmitz (1971)
Automated determination of uric acid, with use of a uricase-peroxidase system.Clinical chemistry, 17 12
-
T. Stadtman, A. Cherkes, C. Anfinsen (1954)
Studies on the microbiological degradation of cholesterol.The Journal of biological chemistry, 206 2
-
The development of an enzymatic technique for the assay of cholesterol in biological fluids -
W. Werner, H. Rey, H. Wielinger (1970)
Über die Eigenschaften eines neuen Chromogens für die Blutzuckerbestimmung nach der GOD/POD-MethodeFresenius' Zeitschrift für analytische Chemie, 252
-
Abell Ll, B. Levy, B. Brodie, F. Kendall (1952)
A simplified method for the estimation of total cholesterol in serum and demonstration of its specificity.The Journal of biological chemistry, 195 1
-
P. Trinder (1969)
Determination of Glucose in Blood Using Glucose Oxidase with an Alternative Oxygen AcceptorAnnals of Clinical Biochemistry, 6
-
(1949)
The ability of soil organisms to decompose steroids -
G. Turfitt (1944)
The microbiological degradation of steroids: 2. Oxidation of cholesterol by Proactinomyces spp.The Biochemical journal, 38 5
-
W. Brown (1961)
ERRORS IN THE DETERMINATION OF SERUM CHOLESTEROLImmunology and Cell Biology, 39
-
(1952)
J. Biol. Chem -
D. Tonks (1967)
The estimation of cholesterol in serum:A classification and critical review of methods*Clinical Biochemistry, 1
-
W. Richmond (1973)
Preparation and properties of a cholesterol oxidase from Nocardia sp. and its application to the enzymatic assay of total cholesterol in serum.Clinical chemistry, 19 12
-
J. Hyun, H. Kothari, E. Herm, J. Mortenson, C. Treadwell, G. Vahouny (1969)
Purification and properties of pancreatic juice cholesterol esterase.The Journal of biological chemistry, 244 7
-
(1958)
N. Y
- Publisher
- Oxford University Press
- Copyright
- © 1974 The American Association of Clinical Chemists, Inc.
- ISSN
- 0009-9147
- eISSN
- 1530-8561
- DOI
- 10.1093/clinchem/20.4.470
- Publisher site
- See Article on Publisher Site
Abstract
Abstract An enzymatic method is described for determination of total serum cholesterol by use of a single aqueous reagent. The method requires no prior treatment of sample and the calibration curve is linear to 600 mg/dl. Cholesterol esters are hydrolyzed to free cholesterol by cholesterol ester hydrolase (EC 3.1.1.13). The free cholesterol produced is oxidized by cholesterol oxidase to cholest-4-en-3-one with the simultaneous production of hydrogen peroxide, which oxidatively couples with 4-aminoantipyrine and phenol in the presence of peroxidase to yield a chromogen with maximum absorption at 500 nm. The method is reproducible, and the results correlate well with those obtained by automated Liebermann—Burchard procedures (AA-2 and SMA 12/60) and the method of Abell et al. The present method affords better specificity than those previously reported and has excellent precision. This content is only available as a PDF. © 1974 The American Association of Clinical Chemists, Inc. This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)
Journal
Clinical Chemistry – Oxford University Press
Published: Apr 1, 1974