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Molecular cloning and expression of the human melanocyte stimulating hormone receptor cDNA

Molecular cloning and expression of the human melanocyte stimulating hormone receptor cDNA Melanocytes and melanoma cells are known to possess receptors for melanocyte stimulating hormone (MSH). A cDNA clone, designated IID, has been isolated from human melanoma cells and encodes a MSH receptor. The cloned cDNA encodes a 317 amino acid protein with transmembrane topography characteristics of a G‐protein‐coupled receptor, but it does not show striking similarity to already published sequences of other G‐protein‐coupled receptors. When IID cDNA is expressed in COS‐7 cells, it binds an 125I‐labelled MSH analogue (NDP‐MSH) in a specific manner. The bound ligand could be displaced by melanotropic peptides, α‐MSH, β‐MSH, γ‐MSH and ACTH (adrenocorticotropic hormone), but not by the non‐melanotropic peptide, β‐endorphine. This is the first report of the cloning of a receptor gene of the melanotropin receptor family. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png FEBS Letters Wiley

Molecular cloning and expression of the human melanocyte stimulating hormone receptor cDNA

Chhajlani, Vijay; Wikberg, Jarl E.S.
FEBS Letters , Volume 309 (3) – Sep 14, 1992
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References (19)

Publisher
Wiley
Copyright
© 2015 Federation of European Biochemical Societies
eISSN
1873-3468
DOI
10.1016/0014-5793(92)80820-7
Publisher site
See Article on Publisher Site

Abstract

Melanocytes and melanoma cells are known to possess receptors for melanocyte stimulating hormone (MSH). A cDNA clone, designated IID, has been isolated from human melanoma cells and encodes a MSH receptor. The cloned cDNA encodes a 317 amino acid protein with transmembrane topography characteristics of a G‐protein‐coupled receptor, but it does not show striking similarity to already published sequences of other G‐protein‐coupled receptors. When IID cDNA is expressed in COS‐7 cells, it binds an 125I‐labelled MSH analogue (NDP‐MSH) in a specific manner. The bound ligand could be displaced by melanotropic peptides, α‐MSH, β‐MSH, γ‐MSH and ACTH (adrenocorticotropic hormone), but not by the non‐melanotropic peptide, β‐endorphine. This is the first report of the cloning of a receptor gene of the melanotropin receptor family.

Journal

FEBS Letters – Wiley

Published: Sep 14, 1992

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