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Identification of HLA‐B35, B53, B18, B5, B78, and B17 alleles by the polymerase chain reaction using sequence‐specific primers (PCR‐SSP)

Identification of HLA‐B35, B53, B18, B5, B78, and B17 alleles by the polymerase chain reaction... ISSN 0001-2Rlj Brief Communication Identification of HLA-B35, B53, B18, B5, B78, and B17 alleles by the polymerase chain reaction usmg sequence-speciIic pnmers (PCR-SSP) M. G. Guttridge, C. Burr, P. T. Klouda. Identification of HLA-B35, B53, B18, B5. B78 and B17 alleles bv the Dolvmerase chain reaction using sequence-specificprimers (PCR-SSP). Tissue Antigens 1994: 44: 43-46. 0 Munksgaard, 1994 , , I I M. G. Guttridge, C. Burr and P. T. Klouda UK Transplant Support Service Authority, Stoke Gifford, Bristol, U.K. Received 18 October, 1993, revised, accepted for publication 22 February 1994 The polymorphisms of the human major histocompatibility complex class I and I1 molecules, encoded by the HLA-A, B, Cw, DR and DQ genes, have been studied extensively using serological methods. A major drawback of serology is that monospecific antisera are rare, making the discrimination of some antigens difficult. Many HLA class I antigens may be resolved by one-dimensional isoelectric fo) cusing (1D-IEF) (1, 2, but for some that share ID-IEF band positions, including B35 and B53; B57 and B58, an alternative method is required. The amplification of HLA genes by the polymerase chain reaction using sequence-specific primers (PCR-SSP) provides a rapid and reliable method for typing HLA-A, Cw, http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Tissue Antigens Wiley

Identification of HLA‐B35, B53, B18, B5, B78, and B17 alleles by the polymerase chain reaction using sequence‐specific primers (PCR‐SSP)

Guttridge, M. G.; Burr, C.; Klouda, P. T.
Tissue Antigens , Volume 44 (1) – Jul 1, 1994
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References (9)

Publisher
Wiley
Copyright
Copyright © 1994 Munksgaard
ISSN
0001-2815
eISSN
1399-0039
DOI
10.1111/j.1399-0039.1994.tb02355.x
Publisher site
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Abstract

ISSN 0001-2Rlj Brief Communication Identification of HLA-B35, B53, B18, B5, B78, and B17 alleles by the polymerase chain reaction usmg sequence-speciIic pnmers (PCR-SSP) M. G. Guttridge, C. Burr, P. T. Klouda. Identification of HLA-B35, B53, B18, B5. B78 and B17 alleles bv the Dolvmerase chain reaction using sequence-specificprimers (PCR-SSP). Tissue Antigens 1994: 44: 43-46. 0 Munksgaard, 1994 , , I I M. G. Guttridge, C. Burr and P. T. Klouda UK Transplant Support Service Authority, Stoke Gifford, Bristol, U.K. Received 18 October, 1993, revised, accepted for publication 22 February 1994 The polymorphisms of the human major histocompatibility complex class I and I1 molecules, encoded by the HLA-A, B, Cw, DR and DQ genes, have been studied extensively using serological methods. A major drawback of serology is that monospecific antisera are rare, making the discrimination of some antigens difficult. Many HLA class I antigens may be resolved by one-dimensional isoelectric fo) cusing (1D-IEF) (1, 2, but for some that share ID-IEF band positions, including B35 and B53; B57 and B58, an alternative method is required. The amplification of HLA genes by the polymerase chain reaction using sequence-specific primers (PCR-SSP) provides a rapid and reliable method for typing HLA-A, Cw,

Journal

Tissue Antigens – Wiley

Published: Jul 1, 1994

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