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Use of immunohistologic and in situ hybridization techniques in the examination of sacroiliac joint biopsy specimens from patients with ankylosing spondylitis

Use of immunohistologic and in situ hybridization techniques in the examination of sacroiliac... Objective. To investigate mechanisms involved in inflammation and new bone formation in the sacroiliac (SI) joints of patients with ankylosing spondylitis (AS). Patients and methods. Computed tomography–assisted biopsy of the SI joint was performed in 5 patients with AS with a mean disease duration of 4.5 years and radiographic stage 2–3 disease. Immuno‐histologic studies were performed with the alkaline phosphatase–anti–alkaline phosphatase technique, and cytokine messenger RNA (mRNA) was detected by in situ hybridization. Results. Dense cellular infiltrates with varying amounts of CD3+ cells (mean ± SD 53.3 ± 24.1%), CD4+ cells (29.7 ± 17.6%), CD8+ cells (15.8 ± 11.4%), CD14+ cells (23.6 ± 16.9%), CD45RO+ cells (48.4 ± 23.6%), and CD45RA+ cells (4.5 ± 2.9%) were found in the synovial portion of the SI joints of all 5 patients. In these infiltrates a high amount of tumor necrosis factor α (TNFα) mRNA and, near the site of new bone formation, a lower amount of transforming growth factor β (TGF β) mRNA, were detected, while no message for interleukin‐1 was found in the 3 patients examined by this technique. Conclusion. The presence of T cells and macrophages was demonstrated in cellular infiltrates in the SI joints of 5 patients with active AS. The finding of abundant TNFα message in these joints could have implications regarding potential immunotherapeutic approaches to this disease. TGFβ might be involved in new bone formation in AS. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Arthritis & Rheumatism Wiley

Use of immunohistologic and in situ hybridization techniques in the examination of sacroiliac joint biopsy specimens from patients with ankylosing spondylitis

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References (44)

Publisher
Wiley
Copyright
Copyright © 1995 American College of Rheumatology
ISSN
0004-3591
eISSN
1529-0131
DOI
10.1002/art.1780380407
Publisher site
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Abstract

Objective. To investigate mechanisms involved in inflammation and new bone formation in the sacroiliac (SI) joints of patients with ankylosing spondylitis (AS). Patients and methods. Computed tomography–assisted biopsy of the SI joint was performed in 5 patients with AS with a mean disease duration of 4.5 years and radiographic stage 2–3 disease. Immuno‐histologic studies were performed with the alkaline phosphatase–anti–alkaline phosphatase technique, and cytokine messenger RNA (mRNA) was detected by in situ hybridization. Results. Dense cellular infiltrates with varying amounts of CD3+ cells (mean ± SD 53.3 ± 24.1%), CD4+ cells (29.7 ± 17.6%), CD8+ cells (15.8 ± 11.4%), CD14+ cells (23.6 ± 16.9%), CD45RO+ cells (48.4 ± 23.6%), and CD45RA+ cells (4.5 ± 2.9%) were found in the synovial portion of the SI joints of all 5 patients. In these infiltrates a high amount of tumor necrosis factor α (TNFα) mRNA and, near the site of new bone formation, a lower amount of transforming growth factor β (TGF β) mRNA, were detected, while no message for interleukin‐1 was found in the 3 patients examined by this technique. Conclusion. The presence of T cells and macrophages was demonstrated in cellular infiltrates in the SI joints of 5 patients with active AS. The finding of abundant TNFα message in these joints could have implications regarding potential immunotherapeutic approaches to this disease. TGFβ might be involved in new bone formation in AS.

Journal

Arthritis & Rheumatism – Wiley

Published: Apr 1, 1995

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