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Nucleolar transcription factor hUBF contains a DNA-binding motif with homology to HMG proteins

Nucleolar transcription factor hUBF contains a DNA-binding motif with homology to HMG proteins The eukaryotic upstream binding factor (DBF), recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA poly-merase I through cooperative interactions with the species-specific factor, SL1. Isolation of complementary DNA clones and sequence analysis reveals similarities between DNA binding domains of human UBF (hUBF) and high mobility group (HMG) protein 1. Expression, cellular localization and in vitro transcription studies establish that cloned hUBF encodes a nucleolar factor that binds specifically to the upstream control element and core of the rRNA gene promoter to activate transcription in a binding site-dependent manner. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nature Springer Journals

Nucleolar transcription factor hUBF contains a DNA-binding motif with homology to HMG proteins

Nature , Volume 344 (6269) – Apr 26, 1990

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References (50)

Publisher
Springer Journals
Copyright
Copyright © Springer Nature Limited 1990
Subject
Science, Humanities and Social Sciences, multidisciplinary; Science, Humanities and Social Sciences, multidisciplinary; Science, multidisciplinary
ISSN
0028-0836
eISSN
1476-4687
DOI
10.1038/344830a0
Publisher site
See Article on Publisher Site

Abstract

The eukaryotic upstream binding factor (DBF), recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA poly-merase I through cooperative interactions with the species-specific factor, SL1. Isolation of complementary DNA clones and sequence analysis reveals similarities between DNA binding domains of human UBF (hUBF) and high mobility group (HMG) protein 1. Expression, cellular localization and in vitro transcription studies establish that cloned hUBF encodes a nucleolar factor that binds specifically to the upstream control element and core of the rRNA gene promoter to activate transcription in a binding site-dependent manner.

Journal

NatureSpringer Journals

Published: Apr 26, 1990

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