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Engineered Zinc Finger ProteinsBeyond DNA: Zinc Finger Domains as RNA-Binding Modules

Engineered Zinc Finger Proteins: Beyond DNA: Zinc Finger Domains as RNA-Binding Modules [Over the last 25 years, we have learned that many structural classes of zinc-binding domains (zinc fingers, ZFs) exist and it has become clear that the molecular functions of these domains are by no means limited to the sequence-specific recognition of double-stranded DNA. For example, ZFs can act as protein recognition or RNA-binding modules, and some domains can exhibit more than one function. In this chapter we describe the progress that has been made in understanding the role of ZF domains as RNA-recognition modules, and we speculate about both the prevalence of such functions and the prospects for creating designer ZFs that target RNA.] http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

Engineered Zinc Finger ProteinsBeyond DNA: Zinc Finger Domains as RNA-Binding Modules

Part of the Methods in Molecular Biology Book Series (volume 649)
Editors: Mackay, Joel P.; Segal, David J.

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References (65)

Publisher
Humana Press
Copyright
© Springer Science + Business Media, LLC 2010
ISBN
978-1-60761-752-5
Pages
479–491
DOI
10.1007/978-1-60761-753-2_29
Publisher site
See Chapter on Publisher Site

Abstract

[Over the last 25 years, we have learned that many structural classes of zinc-binding domains (zinc fingers, ZFs) exist and it has become clear that the molecular functions of these domains are by no means limited to the sequence-specific recognition of double-stranded DNA. For example, ZFs can act as protein recognition or RNA-binding modules, and some domains can exhibit more than one function. In this chapter we describe the progress that has been made in understanding the role of ZF domains as RNA-recognition modules, and we speculate about both the prevalence of such functions and the prospects for creating designer ZFs that target RNA.]

Published: Jun 25, 2010

Keywords: Zinc fingers; RNA-binding domain; protein interaction domain; protein design

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