/lp/oxford-university-press/simplified-method-for-measuring-sex-hormone-binding-globulin-TISll0d0lM
References (25)
-
G. Moll, R. Rosenfield (1979)
Testosterone binding and free plasma androgen concentrations under physiological conditons: chararacterization by flow dialysis technique.The Journal of clinical endocrinology and metabolism, 49 5
-
W. Rosner, S. Deakins (1968)
Testosterone-binding globulins in human plasma: studies on sex distribution and specificity.The Journal of clinical investigation, 47 9
-
M. Solomon, M. Iqbal, M. Dalton, S. Jeffcoate, J. Ginsburg (1979)
SEX-HORMONE-BINDING GLOBULIN: AN ADDITIONAL TEST FOR OVULATORY FUNCTIONThe Lancet, 313
-
P. Corvol, A. Chrambach, D. Rodbard, C. Bardin (1971)
Physical properties and binding capacity of testosterone-estradiol-binding globulin in human plasma, determined by polyacrylamide gel electrophoresis.The Journal of biological chemistry, 246 11
-
B. Blank, A. Attanasio, K. Rager, D. Gupta (1978)
Determination of serum sex hormone binding globulin (SHBG) in preadolescent and adolescent boys.Journal of steroid biochemistry, 9 2
-
J. Gueriguian, W. Pearlman (1968)
Some properties of a testosterone-binding component of human pregnancy serum.The Journal of biological chemistry, 243 20
-
H. Baelen, W. Heyns, E. Schonne, P. Moor (1968)
An estradiol binding globulin in human serum: partial purification.Annales d'endocrinologie, 29
-
C. Mercier-Bodard, A. Alfsen, E. Baulieu (1970)
Sex steroid binding plasma protein (SBP).Acta endocrinologica. Supplementum, 147
-
D. Anderson, D. Anderson, D. Anderson, Bill Lasley, Bill Lasley, Bill Lasley, Richard Fisher, Richard Fisher, Richard Fisher, J. Shepherd, J. Shepherd, J. Shepherd, L. Newman, L. Newman, L. Newman, Andrew Hendrickx (1976)
TRANSPLACENTAL GRADIENTS OF SEX‐HORMONE‐BINDING GLOBULIN IN HUMAN AND SIMIAN PREGNANCYClinical Endocrinology, 5
-
C. Burke (1969)
Accurate measurement of steroid-protein binding by steady-state gel filtration.Biochimica et biophysica acta, 176 2
-
O. Steeno, W. Heyns, H. Baelen, P. Moor (1968)
Testosterone binding in human plasma.Annales d'endocrinologie, 29
-
A. Vermeulen, L. Verdonck, M. Straeten, N. Orie (1969)
Capacity of the Testosterone-Binding Globulin in Human Plasma and Influence of Specific Binding of Testosterone on Its Metabolic Clearance RateThe Journal of Clinical Endocrinology and Metabolism, 29
-
M. Iqbal, M. Johnson (1977)
Study of steroid-protein binding by a novel "two-tier" column employing Cibacron Blue F3G-A-Sepharose 4B. I-Sex hormone binding globulin.Journal of steroid biochemistry, 8 9
-
W. Pearlman, O. Crépy, M. Murphy (1967)
Testosterone-binding levels in the serum of women during the normal menstrual cycle, pregnancy, and the post-partum period.The Journal of clinical endocrinology and metabolism, 27 7
-
Dan Tulchinsky, Inder Chopra (1973)
Competitive ligand-binding assay for measurement of sex hormone-binding globulin (SHBG).The Journal of clinical endocrinology and metabolism, 37 6
-
D. Anderson, R. Peppiatt, L. Schuster, R. Fisher (1972)
A new method for measurement of sex hormone-binding globulin in plasma and its clinical application.The Journal of endocrinology, 55 2
-
C. Burke, D. Anderson (1972)
Sex-Hormone-Binding Globulin is an Oestrogen AmplifierNature, 240
-
W. Heyns, P. Moor (1971)
Kinetics of Dissociation of 17β-Hydroxysteroids from the Steroid Binding β-Globulin of Human PlasmaThe Journal of Clinical Endocrinology and Metabolism, 32
-
R. Rosenfield (1971)
Plasma testosterone binding globulin and indexes of the concentration of unbound plasma androgens in normal and hirsute subjects.The Journal of clinical endocrinology and metabolism, 32 6
-
G. August, M. Tkachuk, M. Grumbach (1969)
Plasma testosterone-binding affinity and testosterone in umbilical cord plasma, late pregnancy, prepubertal children, and adults.The Journal of clinical endocrinology and metabolism, 29 7
-
G. Moll, R. Rosenfield (1978)
Some limitations of using equilibrium dialysis to study human serum albumin-testosterone interaction.The Journal of clinical endocrinology and metabolism, 46 3
-
W. Rosner (1972)
A simplified method for the quantitative determination of testosterone-estradiol-binding globulin activity in human plasma.The Journal of clinical endocrinology and metabolism, 34 6
-
R. Fisher, D. Anderson, C. Burke (1974)
Simultaneous measurement of unbound testosterone and estradiol fractions in undiluted plasma at 37 degrees C by steady-state gel filtration.Steroids, 24 6
-
A. Vermeulen, L. Verdonck (1968)
Studies on the binding of testosterone to human plasma.Steroids, 11 5
-
W. Heyns, P. Moor (1971)
The binding of 17 -hydroxy-5 -androstan-3-one to the steroid-binding -globulin in human plasma, as studied by means of ammonium sulphate precipitation.Steroids, 18 6
- Publisher
- Oxford University Press
- Copyright
- © 1981 The American Association of Clinical Chemistry
- ISSN
- 0009-9147
- eISSN
- 1530-8561
- DOI
- 10.1093/clinchem/27.7.1277
- Publisher site
- See Article on Publisher Site
Abstract
Abstract We describe a simple, rapid method for measurement of sex-hormone binding globulin. Serial dilutions of pregnancy serum are prepared in serum from males that has been pre-treated by heating to 60 degrees C for 1 h to destroy endogenous binding globulin, which is then determined by a long-used technique to yield a set of "standards." In the assay itself, a fixed amount of [3H]-labeled and unlabeled dihydrotestosterone is incubated with standard or unknown, and the bound fraction precipitated with saturated ammonium sulfate. A plot of percent of the steroid bound vs standard dilution yields a sigmoid curve, from which the results in unknowns can be read by simple extrapolation. Within-assay CVs for pools of serum from men, women, and women in late pregnancy were 6.56, 9.59, and 8.4%, respectively. Between-assay CVs for the same pools were 8.05, 9.5, and 11.5%, respectively. The correlation between results obtained by this method and those of the older technique was 0.95 for samples from non-pregnant subjects and 0.73 for those from pregnant women. Our procedure is simpler and faster than previous methods and accurately measures the differences in the globulin in sera from men, women, and pregnant women. Forty to 50 samples can be assayed in a working day. This content is only available as a PDF. © 1981 The American Association of Clinical Chemistry This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)
Journal
Clinical Chemistry – Oxford University Press
Published: Jul 1, 1981