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Strength-frequency curve for micromagnetic neurostimulation through excitatory postsynaptic potentials (EPSPs) on rat hippocampal neurons and numerical modeling of magnetic microcoil (μcoil)

Strength-frequency curve for micromagnetic neurostimulation through excitatory postsynaptic... Objective. The objective of this study was to measure the effect of micromagnetic stimulation (μMS) on hippocampal neurons, by using single microcoil (μcoil) prototype, magnetic pen (MagPen). MagPen will be used to stimulate the CA3 region magnetically and excitatory post synaptic potential (EPSP) response measurements will be made from the CA1 region. The threshold for micromagnetic neurostimulation as a function of stimulation frequency of the current driving the µcoil will be demonstrated. Finally, the optimal stimulation frequency of the current driving the μcoil to minimize power will be estimated. Approach. A biocompatible, watertight, non-corrosive prototype, MagPen was built, and customized such that it is easy to adjust the orientation of the μcoil and its distance over the hippocampal tissue in an in vitro recording setting. Finite element modeling of the μcoil design was performed to estimate the spatial profiles of the magnetic flux density (in T) and the induced electric fields (in V m−1). The induced electric field profiles generated at different values of current applied to the µcoil can elicit a neuronal response, which was validated by numerical modeling. The modeling settings for the μcoil were replicated in experiments on rat hippocampal neurons. Main results. The preferred orientation of MagPen over the Schaffer Collateral fibers was demonstrated such that they elicit a neuron response. The recorded EPSPs from CA1 region due to μMS at CA3 region were validated by applying tetrodotoxin (TTX). Application of TTX to the hippocampal slice blocked the EPSPs from μMS while after prolonged TTX washout, a partial recovery of the EPSP from μMS was observed. Finally, it was interpreted through numerical analysis that increasing frequency of the current driving the μcoil, led to a decrease in the current amplitude threshold for micromagnetic neurostimulation. Significance. This work reports that micromagnetic neurostimulation can be used to evoke population EPSP responses in the CA1 region of the hippocampus. It demonstrates the strength-frequency curve for µMS and its unique features related to orientation dependence of the µcoils, spatial selectivity and stimulation threshold related to distance dependence. Finally, the challenges related to µMS experiments were studied including ways to overcome them. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neural Engineering IOP Publishing

Strength-frequency curve for micromagnetic neurostimulation through excitatory postsynaptic potentials (EPSPs) on rat hippocampal neurons and numerical modeling of magnetic microcoil (μcoil)

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References (57)

Publisher
IOP Publishing
Copyright
© 2022 IOP Publishing Ltd
ISSN
1741-2560
eISSN
1741-2552
DOI
10.1088/1741-2552/ac4baf
Publisher site
See Article on Publisher Site

Abstract

Objective. The objective of this study was to measure the effect of micromagnetic stimulation (μMS) on hippocampal neurons, by using single microcoil (μcoil) prototype, magnetic pen (MagPen). MagPen will be used to stimulate the CA3 region magnetically and excitatory post synaptic potential (EPSP) response measurements will be made from the CA1 region. The threshold for micromagnetic neurostimulation as a function of stimulation frequency of the current driving the µcoil will be demonstrated. Finally, the optimal stimulation frequency of the current driving the μcoil to minimize power will be estimated. Approach. A biocompatible, watertight, non-corrosive prototype, MagPen was built, and customized such that it is easy to adjust the orientation of the μcoil and its distance over the hippocampal tissue in an in vitro recording setting. Finite element modeling of the μcoil design was performed to estimate the spatial profiles of the magnetic flux density (in T) and the induced electric fields (in V m−1). The induced electric field profiles generated at different values of current applied to the µcoil can elicit a neuronal response, which was validated by numerical modeling. The modeling settings for the μcoil were replicated in experiments on rat hippocampal neurons. Main results. The preferred orientation of MagPen over the Schaffer Collateral fibers was demonstrated such that they elicit a neuron response. The recorded EPSPs from CA1 region due to μMS at CA3 region were validated by applying tetrodotoxin (TTX). Application of TTX to the hippocampal slice blocked the EPSPs from μMS while after prolonged TTX washout, a partial recovery of the EPSP from μMS was observed. Finally, it was interpreted through numerical analysis that increasing frequency of the current driving the μcoil, led to a decrease in the current amplitude threshold for micromagnetic neurostimulation. Significance. This work reports that micromagnetic neurostimulation can be used to evoke population EPSP responses in the CA1 region of the hippocampus. It demonstrates the strength-frequency curve for µMS and its unique features related to orientation dependence of the µcoils, spatial selectivity and stimulation threshold related to distance dependence. Finally, the challenges related to µMS experiments were studied including ways to overcome them.

Journal

Journal of Neural Engineering – IOP Publishing

Published: Feb 1, 2022

Keywords: micromagnetic neurostimulation; microcoil; tetrodotoxin; strength-frequency curve; hippocampus; excitatory post synaptic potential (EPSP); spatial selectivity

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