Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 7-Day Trial for You or Your Team.

Learn More →

Trypsin‐Like Activity in Subgingival Plaque

Trypsin‐Like Activity in Subgingival Plaque Taxonomic screening of subgingival plaque organisms with various enzyme assays have shown that Treponema denticela, Bacteroides gingivalis and an unspeciated Capnocytophaga species possess a trypsin‐like enzyme (TLE) that can be detected by the hydrolysis of N‐benzoyl‐DL‐arginine‐2‐naphthylamide (BANA). As these organisms can be considered to be periodontopathic, it was of interest to determine whether this BANA hydrolyzing enzyme could be detected directly in subgingival plaque samples. Subgingival plaque samples were collected from single sites of known pocket depth, and after dispersal by vortexing, aliquots were incubated overnight with BANA and were counted microscopically. The color reactions were developed with fast garnet, read by the eye and classified as positive (red to red‐orange), negative (yellow) and questionable. In the BANA‐positive plaques, the spirochetes averaged 43% of the microscopic count, whereas in the BANA negative plaques the spirochetes averaged 8% of the microscopic count. The average pocket depth of BANApositive plaques was 6.7 mm, whereas the average pocket depth of BANA‐negative plaques was 4.5 mm. When both of these parameters were combined, the presence of a positive BANA reaction was usually indicative of subgingival plaques containing >34% spirochetes removed from sites that had probing depths of 7 mm or more. Seventy‐one per cent of the plaques removed from untreated periodontal patients were BANA‐positive, while only 8% of the plaques removed from successfully treated patients seen at maintenance recall visits were BANA‐positive. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Periodontology Wiley

Trypsin‐Like Activity in Subgingival Plaque

Download PDF
8 pages

Loading next page...
 
/lp/wiley/trypsin-like-activity-in-subgingival-plaque-XRO00T0umY

References (23)

Publisher
Wiley
Copyright
© American Academy of Periodontology
ISSN
0022-3492
eISSN
1943-3670
DOI
10.1902/jop.1987.58.4.266
pmid
3473222
Publisher site
See Article on Publisher Site

Abstract

Taxonomic screening of subgingival plaque organisms with various enzyme assays have shown that Treponema denticela, Bacteroides gingivalis and an unspeciated Capnocytophaga species possess a trypsin‐like enzyme (TLE) that can be detected by the hydrolysis of N‐benzoyl‐DL‐arginine‐2‐naphthylamide (BANA). As these organisms can be considered to be periodontopathic, it was of interest to determine whether this BANA hydrolyzing enzyme could be detected directly in subgingival plaque samples. Subgingival plaque samples were collected from single sites of known pocket depth, and after dispersal by vortexing, aliquots were incubated overnight with BANA and were counted microscopically. The color reactions were developed with fast garnet, read by the eye and classified as positive (red to red‐orange), negative (yellow) and questionable. In the BANA‐positive plaques, the spirochetes averaged 43% of the microscopic count, whereas in the BANA negative plaques the spirochetes averaged 8% of the microscopic count. The average pocket depth of BANApositive plaques was 6.7 mm, whereas the average pocket depth of BANA‐negative plaques was 4.5 mm. When both of these parameters were combined, the presence of a positive BANA reaction was usually indicative of subgingival plaques containing >34% spirochetes removed from sites that had probing depths of 7 mm or more. Seventy‐one per cent of the plaques removed from untreated periodontal patients were BANA‐positive, while only 8% of the plaques removed from successfully treated patients seen at maintenance recall visits were BANA‐positive.

Journal

Journal of Periodontology – Wiley

Published: Apr 1, 1987

There are no references for this article.