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Alpha‐synuclein aggregation and cell death triggered by energy deprivation and dopamine overload are counteracted by D2/D3 receptor activation

Alpha‐synuclein aggregation and cell death triggered by energy deprivation and dopamine overload... Progressive degeneration and intraneuronal Lewy bodies made of filamentous α‐synuclein (α‐syn) in dopaminergic cells of the nigrostriatal system are characteristics of Parkinson’s disease (PD). Glucose uptake is reduced in some of the brain regions affected by PD neurodegenerative changes. Defects in mitochondrial activity in the substantia nigra have been observed in the brain of patients affected by PD and substantia nigra lesions can induce the onset of a secondary parkinsonism. Thus, energy starvation and consequently metabolic impairment to dopaminergic neurons may be related to the onset of PD. On this line, we evaluated the effect of nutrient starvation, reproduced ‘in vitro’ by glucose deprivation (GD), in primary mesecephalic neuronal cultures and dopaminergic‐differentiated SH‐SY5Y cells, to evaluate if decreased glucose support to dopaminergic cells can lead to mitochondrial damage, neurodegeneration and α‐syn misfolding. Furthermore, we investigated the effect of dopamine (DA) treatment in the presence of a DA‐uptake inhibitor or of the D2/D3 receptor (D2R/D3R) agonist quinpirole on GD‐treated cells, to evaluate the efficacy of these therapeutic compounds. We found that GD induced the formation of fibrillary aggregated α‐syn inclusions containing the DA transporter in dopaminergic cells. These alterations were accompanied by dopaminergic cell death and were exacerbated by DA overload. Conversely, the block of DA uptake and D2R/D3R agonist treatment exerted neuroprotective effects. These data indicate that glucose starvation is likely involved in the induction of PD‐related pathological changes in dopaminergic neurons. These changes may be counteracted by the block of DA uptake and by dopaminergic agonist treatment. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neurochemistry Wiley

Alpha‐synuclein aggregation and cell death triggered by energy deprivation and dopamine overload are counteracted by D2/D3 receptor activation

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References (54)

Publisher
Wiley
Copyright
Copyright © 2008 Wiley Subscription Services
ISSN
0022-3042
eISSN
1471-4159
DOI
10.1111/j.1471-4159.2008.05406.x
pmid
18410503
Publisher site
See Article on Publisher Site

Abstract

Progressive degeneration and intraneuronal Lewy bodies made of filamentous α‐synuclein (α‐syn) in dopaminergic cells of the nigrostriatal system are characteristics of Parkinson’s disease (PD). Glucose uptake is reduced in some of the brain regions affected by PD neurodegenerative changes. Defects in mitochondrial activity in the substantia nigra have been observed in the brain of patients affected by PD and substantia nigra lesions can induce the onset of a secondary parkinsonism. Thus, energy starvation and consequently metabolic impairment to dopaminergic neurons may be related to the onset of PD. On this line, we evaluated the effect of nutrient starvation, reproduced ‘in vitro’ by glucose deprivation (GD), in primary mesecephalic neuronal cultures and dopaminergic‐differentiated SH‐SY5Y cells, to evaluate if decreased glucose support to dopaminergic cells can lead to mitochondrial damage, neurodegeneration and α‐syn misfolding. Furthermore, we investigated the effect of dopamine (DA) treatment in the presence of a DA‐uptake inhibitor or of the D2/D3 receptor (D2R/D3R) agonist quinpirole on GD‐treated cells, to evaluate the efficacy of these therapeutic compounds. We found that GD induced the formation of fibrillary aggregated α‐syn inclusions containing the DA transporter in dopaminergic cells. These alterations were accompanied by dopaminergic cell death and were exacerbated by DA overload. Conversely, the block of DA uptake and D2R/D3R agonist treatment exerted neuroprotective effects. These data indicate that glucose starvation is likely involved in the induction of PD‐related pathological changes in dopaminergic neurons. These changes may be counteracted by the block of DA uptake and by dopaminergic agonist treatment.

Journal

Journal of Neurochemistry – Wiley

Published: Jan 1, 2008

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