Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 7-Day Trial for You or Your Team.

Learn More →

Temperature-dependent aminoglycoside resistance in Stenotrophomonas (Xanthomonas) maltophilia; alterations in protein and lipopolysaccharide with growth temperature

Temperature-dependent aminoglycoside resistance in Stenotrophomonas (Xanthomonas) maltophilia;... Clinical strains of Stenotrophomonas (Xanthomonas) maltophilia often show large, growth temperature-dependent, variations in their susceptibility (TDVS) to aminoglycoside antibiotics. Strains showing more than a fourfold increase in susceptibility between 30° and 37°C (TDVS+strains; n =23) were contrasted with those showing lesser variation (TDVS–strains; n =15) in studies of growth temperature-dependent variation in protein and cell-wall lipopolysaccharide (LPS) electrophoresis patterns in an attempt to determine the mechanism of TDVS. Several proteins showed increased intensity with increasing growth temperature. These comprised bands at c. 65, 55, 42.5, 26 and 21.5 kDa in the whole cell proteins, an outer membrane protein band at c . 21.5 kDa, and cytoplasmic membrane protein bands at c . 42.5 and 27 kDa. Two whole cell protein bands at c . 30 and 24 kDa and three outer membrane protein bands at c . 45, 30 and 24 kDa decreased in intensity with increasing growth temperature. However, there was no correlation with the extent of variation in susceptibility, either in the extent of temperature dependent changes in protein banding patterns, or the presence or absence of specific protein bands. By contrast, temperature-dependent variation in LPS patterns correlated well with TDVS. TDVS+ strains yielded intense ladder patterns of more than 30 discrete bands, and the mean molecular weight of the ladder pattern was markedly higher at growth temperatures ≤30°C, than at ≥37°C. TDVS- strains gave a clearly distinct high mol. wt LPS banding pattern showing fewer, less intense bands and a smaller and less consistent shift in mean molecular weight with temperature. Strains which were clearly resistant at 30° and 37°C, had a high mol. wt. polysaccharide component but an absence of the typical LPS-ladder pattern. We conclude that the temperature-dependent variation in the aminoglycoside susceptibility of this species was not correlated with any detectable change in protein composition, but correlated well with changes in LPS structure. © 1996 The British Society for Antimicrobial Chemotherapy « Previous | Next Article » Table of Contents This Article J. Antimicrob. Chemother. (1996) 37 (4): 665-676. doi: 10.1093/jac/37.4.665 » Abstract Free Full Text (PDF) Free Classifications Original Article Services Article metrics Alert me when cited Alert me if corrected Find similar articles Similar articles in Web of Science Similar articles in PubMed Add to my archive Download citation Request Permissions Disclaimer Citing Articles Load citing article information Citing articles via CrossRef Citing articles via Scopus Citing articles via Web of Science Citing articles via Google Scholar Google Scholar Articles by Rahmati-Bahram, A. Articles by Jackson, S. K. Search for related content PubMed PubMed citation Articles by Rahmati-Bahram, A. Articles by Magee, J. T. Articles by Jackson, S. K. Related Content Load related web page information Share Email this article CiteULike Delicious Facebook Google+ Mendeley Twitter What's this? Search this journal: Advanced » Current Issue November 2015 70 (11) Alert me to new issues The Journal About this journal Free leading articles Editor’s Choice Free Editorials JAC Supplements Rights & Permissions This journal is a member of the Committee on Publication Ethics We are mobile – find out more Journals Career Network Published on behalf of The British Society for Antimicrobial Chemotherapy Impact factor: 5.313 5-Yr impact factor: 4.984 Editor-in-Chief Dr Peter Donnelly View full editorial board For Authors Instructions to authors Online submission instructions Submit Now! Self-archiving policy Open access options for authors - visit Oxford Open This journal enables compliance with the NIH Public Access Policy WhsSvhnOkaAwYG81FJCYgwG7z1LnIP2F true Looking for your next opportunity? Looking for jobs... jQuery_1_11 = jQuery.noConflict(true); Alerting Services Email table of contents Email Advance Access CiteTrack XML RSS feed Corporate Services Advertising sales Reprints Supplements var taxonomies = ("MED00300", "MED00460"); Most Most Read Complicated urinary tract infections: practical solutions for the treatment of multiresistant Gram-negative bacteria The use of fluconazole and itraconazole in the treatment of Candida albicans infections: a review Antibacterial activity of essential oils and their major constituents against respiratory tract pathogens by gaseous contact Determination of minimum inhibitory concentrations Factors impacting on the problem of antibiotic resistance » View all Most Read articles Most Cited Methicillin-resistant Staphylococcus aureus clinical strain with reduced vancomycin susceptibility Determination of minimum inhibitory concentrations Synergy, antagonism, and what the chequerboard puts between them Intercontinental emergence of Escherichia coli clone O25:H4-ST131 producing CTX-M-15 CTX-M: changing the face of ESBLs in Europe » View all Most Cited articles Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department. Online ISSN 1460-2091 - Print ISSN 0305-7453 Copyright © 2015 British Society for Antimicrobial Chemotherapy Oxford Journals Oxford University Press Site Map Privacy Policy Cookie Policy Legal Notices Frequently Asked Questions Other Oxford University Press sites: Oxford University Press Oxford Journals China Oxford Journals Japan Academic & Professional books Children's & Schools Books Dictionaries & Reference Dictionary of National Biography Digital Reference English Language Teaching Higher Education Textbooks International Education Unit Law Medicine Music Online Products & Publishing Oxford Bibliographies Online Oxford Dictionaries Online Oxford English Dictionary Oxford Language Dictionaries Online Oxford Scholarship Online Reference Rights and Permissions Resources for Retailers & Wholesalers Resources for the Healthcare Industry Very Short Introductions World's Classics function fnc_onDomLoaded() { var query_context = getQueryContext(); PF_initOIUnderbar(query_context,":QS:default","","JRN"); PF_insertOIUnderbar(0); }; if (window.addEventListener) { window.addEventListener('load', fnc_onDomLoaded, false); } else if (window.attachEvent) { window.attachEvent('onload', fnc_onDomLoaded); } var gaJsHost = (("https:" == document.location.protocol) ? "https://ssl." : "http://www."); document.write(unescape("%3Cscript src='" + gaJsHost + "google-analytics.com/ga.js' type='text/javascript'%3E%3C/script%3E")); try { var pageTracker = _gat._getTracker("UA-189672-16"); pageTracker._setDomainName(".oxfordjournals.org"); pageTracker._trackPageview(); } catch(err) {} http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Antimicrobial Chemotherapy Oxford University Press

Temperature-dependent aminoglycoside resistance in Stenotrophomonas (Xanthomonas) maltophilia; alterations in protein and lipopolysaccharide with growth temperature

Download PDF
12 pages

Loading next page...
 
/lp/oxford-university-press/temperature-dependent-aminoglycoside-resistance-in-stenotrophomonas-Yo0uPdS0OY

References (36)

Publisher
Oxford University Press
Copyright
Copyright © 2015 British Society for Antimicrobial Chemotherapy
ISSN
0305-7453
eISSN
1460-2091
DOI
10.1093/jac/37.4.665
Publisher site
See Article on Publisher Site

Abstract

Clinical strains of Stenotrophomonas (Xanthomonas) maltophilia often show large, growth temperature-dependent, variations in their susceptibility (TDVS) to aminoglycoside antibiotics. Strains showing more than a fourfold increase in susceptibility between 30° and 37°C (TDVS+strains; n =23) were contrasted with those showing lesser variation (TDVS–strains; n =15) in studies of growth temperature-dependent variation in protein and cell-wall lipopolysaccharide (LPS) electrophoresis patterns in an attempt to determine the mechanism of TDVS. Several proteins showed increased intensity with increasing growth temperature. These comprised bands at c. 65, 55, 42.5, 26 and 21.5 kDa in the whole cell proteins, an outer membrane protein band at c . 21.5 kDa, and cytoplasmic membrane protein bands at c . 42.5 and 27 kDa. Two whole cell protein bands at c . 30 and 24 kDa and three outer membrane protein bands at c . 45, 30 and 24 kDa decreased in intensity with increasing growth temperature. However, there was no correlation with the extent of variation in susceptibility, either in the extent of temperature dependent changes in protein banding patterns, or the presence or absence of specific protein bands. By contrast, temperature-dependent variation in LPS patterns correlated well with TDVS. TDVS+ strains yielded intense ladder patterns of more than 30 discrete bands, and the mean molecular weight of the ladder pattern was markedly higher at growth temperatures ≤30°C, than at ≥37°C. TDVS- strains gave a clearly distinct high mol. wt LPS banding pattern showing fewer, less intense bands and a smaller and less consistent shift in mean molecular weight with temperature. Strains which were clearly resistant at 30° and 37°C, had a high mol. wt. polysaccharide component but an absence of the typical LPS-ladder pattern. We conclude that the temperature-dependent variation in the aminoglycoside susceptibility of this species was not correlated with any detectable change in protein composition, but correlated well with changes in LPS structure. © 1996 The British Society for Antimicrobial Chemotherapy « Previous | Next Article » Table of Contents This Article J. Antimicrob. Chemother. (1996) 37 (4): 665-676. doi: 10.1093/jac/37.4.665 » Abstract Free Full Text (PDF) Free Classifications Original Article Services Article metrics Alert me when cited Alert me if corrected Find similar articles Similar articles in Web of Science Similar articles in PubMed Add to my archive Download citation Request Permissions Disclaimer Citing Articles Load citing article information Citing articles via CrossRef Citing articles via Scopus Citing articles via Web of Science Citing articles via Google Scholar Google Scholar Articles by Rahmati-Bahram, A. Articles by Jackson, S. K. Search for related content PubMed PubMed citation Articles by Rahmati-Bahram, A. Articles by Magee, J. T. Articles by Jackson, S. K. Related Content Load related web page information Share Email this article CiteULike Delicious Facebook Google+ Mendeley Twitter What's this? Search this journal: Advanced » Current Issue November 2015 70 (11) Alert me to new issues The Journal About this journal Free leading articles Editor’s Choice Free Editorials JAC Supplements Rights & Permissions This journal is a member of the Committee on Publication Ethics We are mobile – find out more Journals Career Network Published on behalf of The British Society for Antimicrobial Chemotherapy Impact factor: 5.313 5-Yr impact factor: 4.984 Editor-in-Chief Dr Peter Donnelly View full editorial board For Authors Instructions to authors Online submission instructions Submit Now! Self-archiving policy Open access options for authors - visit Oxford Open This journal enables compliance with the NIH Public Access Policy WhsSvhnOkaAwYG81FJCYgwG7z1LnIP2F true Looking for your next opportunity? Looking for jobs... jQuery_1_11 = jQuery.noConflict(true); Alerting Services Email table of contents Email Advance Access CiteTrack XML RSS feed Corporate Services Advertising sales Reprints Supplements var taxonomies = ("MED00300", "MED00460"); Most Most Read Complicated urinary tract infections: practical solutions for the treatment of multiresistant Gram-negative bacteria The use of fluconazole and itraconazole in the treatment of Candida albicans infections: a review Antibacterial activity of essential oils and their major constituents against respiratory tract pathogens by gaseous contact Determination of minimum inhibitory concentrations Factors impacting on the problem of antibiotic resistance » View all Most Read articles Most Cited Methicillin-resistant Staphylococcus aureus clinical strain with reduced vancomycin susceptibility Determination of minimum inhibitory concentrations Synergy, antagonism, and what the chequerboard puts between them Intercontinental emergence of Escherichia coli clone O25:H4-ST131 producing CTX-M-15 CTX-M: changing the face of ESBLs in Europe » View all Most Cited articles Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department. Online ISSN 1460-2091 - Print ISSN 0305-7453 Copyright © 2015 British Society for Antimicrobial Chemotherapy Oxford Journals Oxford University Press Site Map Privacy Policy Cookie Policy Legal Notices Frequently Asked Questions Other Oxford University Press sites: Oxford University Press Oxford Journals China Oxford Journals Japan Academic & Professional books Children's & Schools Books Dictionaries & Reference Dictionary of National Biography Digital Reference English Language Teaching Higher Education Textbooks International Education Unit Law Medicine Music Online Products & Publishing Oxford Bibliographies Online Oxford Dictionaries Online Oxford English Dictionary Oxford Language Dictionaries Online Oxford Scholarship Online Reference Rights and Permissions Resources for Retailers & Wholesalers Resources for the Healthcare Industry Very Short Introductions World's Classics function fnc_onDomLoaded() { var query_context = getQueryContext(); PF_initOIUnderbar(query_context,":QS:default","","JRN"); PF_insertOIUnderbar(0); }; if (window.addEventListener) { window.addEventListener('load', fnc_onDomLoaded, false); } else if (window.attachEvent) { window.attachEvent('onload', fnc_onDomLoaded); } var gaJsHost = (("https:" == document.location.protocol) ? "https://ssl." : "http://www."); document.write(unescape("%3Cscript src='" + gaJsHost + "google-analytics.com/ga.js' type='text/javascript'%3E%3C/script%3E")); try { var pageTracker = _gat._getTracker("UA-189672-16"); pageTracker._setDomainName(".oxfordjournals.org"); pageTracker._trackPageview(); } catch(err) {}

Journal

Journal of Antimicrobial Chemotherapy – Oxford University Press

Published: Apr 1, 1996

There are no references for this article.