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Tryptophan is a precursor for melatonin and serotonin biosynthesis in in vitro regenerated St. John's wort (Hypericum perforatum L. cv. Anthos) plants

Tryptophan is a precursor for melatonin and serotonin biosynthesis in in vitro regenerated St.... cv. Anthos) is presented. Isotope tracer experiments were performed on plantlets regenerated from thidiazuron-induced stem explants and grown on MS basal medium for 2 months. Radiolabel from 14 C-tryptophan was recovered as 14 C-indoleacetic acid, 14 C-tryptamine, 14 C-5-hydroxytryptophan, 14 C-serotonin and 14 C-melatonin in the treated St. John's wort plantlets. Chromatographic peak identity was confirmed by high performance liquid chromatography-mass spectrometry-mass spectrometry and quantification of melatonin by radioimmunoassay. Significantly more radiolabel was recovered in serotonin relative to melatonin under low light conditions with this ratio being reversed under increased lighting, indicating that the rate of flow through this biosynthetic pathway is regulated, at least in part, by light. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Cell Reports Springer Journals

Tryptophan is a precursor for melatonin and serotonin biosynthesis in in vitro regenerated St. John's wort (Hypericum perforatum L. cv. Anthos) plants

Plant Cell Reports , Volume 19 (7) – Jun 1, 2000

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References (15)

Publisher
Springer Journals
Copyright
Copyright © 2000 by Springer-Verlag Berlin Heidelberg
Subject
Legacy
ISSN
0721-7714
eISSN
1432-203X
DOI
10.1007/s002990000206
Publisher site
See Article on Publisher Site

Abstract

cv. Anthos) is presented. Isotope tracer experiments were performed on plantlets regenerated from thidiazuron-induced stem explants and grown on MS basal medium for 2 months. Radiolabel from 14 C-tryptophan was recovered as 14 C-indoleacetic acid, 14 C-tryptamine, 14 C-5-hydroxytryptophan, 14 C-serotonin and 14 C-melatonin in the treated St. John's wort plantlets. Chromatographic peak identity was confirmed by high performance liquid chromatography-mass spectrometry-mass spectrometry and quantification of melatonin by radioimmunoassay. Significantly more radiolabel was recovered in serotonin relative to melatonin under low light conditions with this ratio being reversed under increased lighting, indicating that the rate of flow through this biosynthetic pathway is regulated, at least in part, by light.

Journal

Plant Cell ReportsSpringer Journals

Published: Jun 1, 2000

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