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A single short session of media perfusion induces osteogenesis in hBMSCs cultured in porous scaffolds, dependent on cell differentiation stage

A single short session of media perfusion induces osteogenesis in hBMSCs cultured in porous... ARTICLE A Single Short Session of Media Perfusion Induces Osteogenesis in hBMSCs Cultured in Porous Scaffolds, Dependent on Cell Differentiation Stage 1 2 1 Joanna Filipowska, Gwendolen C. Reilly, Anna M. Osyczka Faculty of Biology and Earth Sciences, Department of Cell Biology and Imaging, Jagiellonian University, 30-387 Krakow, Malopolska, Poland; telephone: þ4812 664 5342; fax: þ4812 664 5099; e-mail: [email protected] Department of Materials Science and Engineering, INSIGNEO Institute for In Silico Medicine, University of Sheffield, Sheffield, United Kingdom Introduction ABSTRACT: Perfusing culture media through porous cell-seeded Modern tissue engineering combines cells, growth and differentia- scaffolds is now a common approach within many tissue engineering tion factors, extracellular matrix-imitating scaffolds, and mechanical strategies. Human bone-marrow derived mesenchymal stem cells stimulation. The latter has gained growing attention, as exposing (hBMSC) are a clinically valuable source of osteoprogenitors that cells in vitro provides important mechanical cues similar to those the respond to mechanical stimuli. However, the optimal mechanical conditions for their osteogenic stimulation in vitro have not been cells may experience in vivo. Mesenchymal stem cells located in the defined. Whereas the effects of short durations of media fluid flow have bone marrow of either healthy or impaired, for example fractured been http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biotechnology and Bioengineering Wiley

A single short session of media perfusion induces osteogenesis in hBMSCs cultured in porous scaffolds, dependent on cell differentiation stage

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References (49)

Publisher
Wiley
Copyright
© 2016 Wiley Periodicals, Inc.
ISSN
0006-3592
eISSN
1097-0290
DOI
10.1002/bit.25937
pmid
26806539
Publisher site
See Article on Publisher Site

Abstract

ARTICLE A Single Short Session of Media Perfusion Induces Osteogenesis in hBMSCs Cultured in Porous Scaffolds, Dependent on Cell Differentiation Stage 1 2 1 Joanna Filipowska, Gwendolen C. Reilly, Anna M. Osyczka Faculty of Biology and Earth Sciences, Department of Cell Biology and Imaging, Jagiellonian University, 30-387 Krakow, Malopolska, Poland; telephone: þ4812 664 5342; fax: þ4812 664 5099; e-mail: [email protected] Department of Materials Science and Engineering, INSIGNEO Institute for In Silico Medicine, University of Sheffield, Sheffield, United Kingdom Introduction ABSTRACT: Perfusing culture media through porous cell-seeded Modern tissue engineering combines cells, growth and differentia- scaffolds is now a common approach within many tissue engineering tion factors, extracellular matrix-imitating scaffolds, and mechanical strategies. Human bone-marrow derived mesenchymal stem cells stimulation. The latter has gained growing attention, as exposing (hBMSC) are a clinically valuable source of osteoprogenitors that cells in vitro provides important mechanical cues similar to those the respond to mechanical stimuli. However, the optimal mechanical conditions for their osteogenic stimulation in vitro have not been cells may experience in vivo. Mesenchymal stem cells located in the defined. Whereas the effects of short durations of media fluid flow have bone marrow of either healthy or impaired, for example fractured been

Journal

Biotechnology and Bioengineering – Wiley

Published: Aug 1, 2016

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