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Comparison of nuclear hydration in boar spermatozoa before and after freeze‐thawing: Contrast analysis of cells embedded in bromide‐labeled medium

Comparison of nuclear hydration in boar spermatozoa before and after freeze‐thawing: Contrast... Epon labeled with bromide was used to embed ejaculated and freeze‐thawed spermatozoa, with the hypothesis that it replaces most of cell water. Image analysis of relative contrasts between sperm nuclei and the surrounding medium revealed that when used in low concentrations, bromide is mostly absorbed to the nuclear structures. For higher concentrations, the chromatin is saturated, and the increase in contrast can be used to calculate relative differences in the hydration of nuclei. Boar sperm nuclei are more hydrated after freeze‐thawing than before. © 1992 Wiley‐Liss, Inc. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Microscopy Research and Technique Wiley

Comparison of nuclear hydration in boar spermatozoa before and after freeze‐thawing: Contrast analysis of cells embedded in bromide‐labeled medium

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References (28)

Publisher
Wiley
Copyright
Copyright © 1992 Wiley Subscription Services, Inc., A Wiley Company
ISSN
1059-910X
eISSN
1097-0029
DOI
10.1002/jemt.1070210308
pmid
1606319
Publisher site
See Article on Publisher Site

Abstract

Epon labeled with bromide was used to embed ejaculated and freeze‐thawed spermatozoa, with the hypothesis that it replaces most of cell water. Image analysis of relative contrasts between sperm nuclei and the surrounding medium revealed that when used in low concentrations, bromide is mostly absorbed to the nuclear structures. For higher concentrations, the chromatin is saturated, and the increase in contrast can be used to calculate relative differences in the hydration of nuclei. Boar sperm nuclei are more hydrated after freeze‐thawing than before. © 1992 Wiley‐Liss, Inc.

Journal

Microscopy Research and Technique – Wiley

Published: Jan 1, 1992

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