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Determination of Selected Organochlorine Pesticides and Polychlorinated Biphenyls in Human Serum

Determination of Selected Organochlorine Pesticides and Polychlorinated Biphenyls in Human Serum Abstract A method is presented that can be used to determine the residue level of certain chlorinated pesticides and polychlorinated biphenyls (as Aroclor 1260) in serum. The method involves the following: (1) extraction of denatured serum with organic solvents; (2) elution of the organic extract through micro-Florisil®† columns to obtain two fractions; (3) acid treatment of the less polar Florisil fraction and its subsequent elution through deactivated silica gel to obtain two fractions; and (4) analysis of all three fractions using gas-liquid chromatography with electron capture detection. The method produced in vitro recoveries for 10 pesticides spiked in the range of 1–10.7 ppb of 50.4% to 121.6%, and in the range of 4.98–21 ppb, recoveries ranged from 47.7 to 112.6. In vivo “recoveries” of Aroclor 1260 averaged 104.8% and 92.3% for concentration levels of ∼ 10 and ∼ 30 ppb, respectively. The method could not be compared with the more commonly used hexane extraction technique because of the deleterious effect these extracts had on the gas chromatographic system. * Partial funding for this work was provided by the Agency for Toxic Substances and Disease Registry, Public Health Service, U.S. Department of Health and Human Services, from the Comprehensive Environmental Response, Compensation, and Liability Act trust fund (Superfund), through an interagency agreement with the Environmental Protection Agency. This content is only available as a PDF. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Analytical Toxicology Oxford University Press

Determination of Selected Organochlorine Pesticides and Polychlorinated Biphenyls in Human Serum

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References (16)

Publisher
Oxford University Press
ISSN
0146-4760
eISSN
1945-2403
DOI
10.1093/jat/14.3.137
Publisher site
See Article on Publisher Site

Abstract

Abstract A method is presented that can be used to determine the residue level of certain chlorinated pesticides and polychlorinated biphenyls (as Aroclor 1260) in serum. The method involves the following: (1) extraction of denatured serum with organic solvents; (2) elution of the organic extract through micro-Florisil®† columns to obtain two fractions; (3) acid treatment of the less polar Florisil fraction and its subsequent elution through deactivated silica gel to obtain two fractions; and (4) analysis of all three fractions using gas-liquid chromatography with electron capture detection. The method produced in vitro recoveries for 10 pesticides spiked in the range of 1–10.7 ppb of 50.4% to 121.6%, and in the range of 4.98–21 ppb, recoveries ranged from 47.7 to 112.6. In vivo “recoveries” of Aroclor 1260 averaged 104.8% and 92.3% for concentration levels of ∼ 10 and ∼ 30 ppb, respectively. The method could not be compared with the more commonly used hexane extraction technique because of the deleterious effect these extracts had on the gas chromatographic system. * Partial funding for this work was provided by the Agency for Toxic Substances and Disease Registry, Public Health Service, U.S. Department of Health and Human Services, from the Comprehensive Environmental Response, Compensation, and Liability Act trust fund (Superfund), through an interagency agreement with the Environmental Protection Agency. This content is only available as a PDF.

Journal

Journal of Analytical Toxicology – Oxford University Press

Published: May 1, 1990

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