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Differential expression of the calcium-binding proteins MRP8 and MRP14 in granulomatous conditions: an immunohistochemical study

Differential expression of the calcium-binding proteins MRP8 and MRP14 in granulomatous... SUMMARY MRP14 and MRP8 are well-characterized calcium-binding proteins present in myeloid cells and mononuclear phagocytes. These antigens can easily be visualized in paraffin-embedded tissue, making use of monospecific polyclonal antibodies. This study evaluates MRP14 and MRP8 expression in mononuclear phagocytes in various granulomatous conditions. MRP14 is strongly expressed in all granulomatous conditions. MRP8 is variably expressed. Mononuclear phagocytes in granulomas of foreign body type, cat-scratch discase and erythema nodosum strongly express MRP8. In contrast, MRP8 expression is weak or absent in mononuclear phagocytes of sarcoidosis and tuberculosis. These results show differences in immunophenotype between non-phagocytic mononuclear phagocytes in delayed hypersensitivity type granulomas and phagocytic mononuclear phagocytes in non-hypersensitivity and non-immunological granulomas. granuloma, immunohistochemistry, delayed hypersensitivity REFERENCES Adams , T.E. , Bodmer , J.G. & Boomer , W.F. ( 1983 ) Production and characterization of monoclonal antibodies recognizing the chain subunits of human Ia allogens . Immunology , 50 , 613 . Google Scholar PubMed OpenURL Placeholder Text WorldCat Branutzaeg , P. , Dale , I. Fagerhol , M. ( 1987 ) Distribution of a formalin-resistant myelomonocytic antigen in human tissues. I. Comparison with other leucocyte markers by paired immunofluorescence and immunoenzyme staining . Am. J. clin. Pathol. 87 , 681 . Google Scholar Crossref Search ADS PubMed WorldCat Dale , I. , Brandtzaeg , P., Fagerhol , M.K. & Scott , H. ( 1985 ) Distribution of a new myelomonocytic antigen (L1) in human peripheral blood leukocytes. Immunofluorescence and immunoperoxidase staining features in comparison with lysozyme and lactoferrin . Am. J. clin. Pathol. 84 , 24 . Google Scholar Crossref Search ADS PubMed WorldCat Epstein , W.L. ( 1980 ) Foreign body granulomas . In Basic and Clinical Aspects of Granulomatous Diseases (ed. by D. L. Horns & T. Yoshida) p. 181 . Elsevier , Holland . Google Scholar Google Preview OpenURL Placeholder Text WorldCat COPAC Flavell , D. , Jones , D. & Wright , D. ( 1987 ) Identification of tissue histiocytes on paraffin sections by a new monoclonal antibody . J. Histochem. Cytochem. 35 , 1217 . Google Scholar Crossref Search ADS PubMed WorldCat Hsu , S.M. , Raine , L. & Fanger , H. ( 1981 ) The use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase technique: a comparison between ABC and unlabelled antibody (PAP) procedures . J. Histochem. Cytochem. 29 , 577 . Google Scholar Crossref Search ADS PubMed WorldCat Kaufmann , S. & Flesch , I. ( 1988 ) The role of T cell macrophage interactions in tuberculosis . Springer Semin. Immunopathol. 10 , 337 . Google Scholar Crossref Search ADS PubMed WorldCat Mlnro , C.S. , Campbell , D.A., Collings , L.A. & Poulter , L.W. ( 1987 ) Monoclonal antibodies distinguish macrophages and epitheliod cells in sarcoidosis and leprosy . Clin. exp. Immunol. 68 , 282 . Google Scholar PubMed OpenURL Placeholder Text WorldCat Niemi , K.M. , Förström , L., Hannuksela , M. & Mlstakallio , K.K. ( 1977 ) Nodules on the legs . Acta dermatovenerol. 57 , 145 . Google Scholar OpenURL Placeholder Text WorldCat Odink , K. , Cerletti , N., Brüggen , J., Clerc , R.G., Tarcsay , L., Zwadlo , G., Gerhards , G., Schlegel , R. & Sorg , C. ( 1987 ) Two calcium-binding proteins in infiltrate macrophages of rheumatoid arthritis . Nature. 330 , 80 . Google Scholar Crossref Search ADS PubMed WorldCat Poulter , L. , Campbell , D., Munro , C. & Janossy , G. ( 1986 ) Discrimination of human macrophages and dendritic cells using monoclonal antibodies . Scand. J. Immunol. 24 , 351 . Google Scholar Crossref Search ADS PubMed WorldCat Pulford , K. , Rlgney , E., Jones , M., Stross , P., Micklem , K., Gatter , K. & Mason , D.Y. ( 1989 ) KP1—a new monoclonal antibody detecting a monocyte/macrophage associated antigen in routinely processed tissue sections . J. clin. Palhol. 42 , 414 . Google Scholar Crossref Search ADS WorldCat Seal , R. , Edwards , J. & Hayes , M. ( 1975 ) The response of the lung to inhaled antigens . Prog. respir. Res. 8 , 125 . Google Scholar Crossref Search ADS WorldCat Semenzato , G. ( 1988 ) Recent advances on sarcoid immunology . Sarcoidosis. 5 , 5 . Google Scholar PubMed OpenURL Placeholder Text WorldCat Spector , W.G. & Heesom , N. ( 1969 ) The production of granulomata by antigen antibody complexes . J. Pathol. 98 , 31 . Google Scholar Crossref Search ADS PubMed WorldCat van den Oord , J.J. , de Wolf-Peeters , C., Facchetti , F. & Desmet , V. J. ( 1984 ) Immunohistochemical analysis of tuberculous and sarcoidal lymphadentis . Hum. Pathol. 15 , 559 . Google Scholar Crossref Search ADS PubMed WorldCat Warren , K.S. ( 1976 ) A functional classification of granulomatous inflammation . Ann. NY Acad. Sci. 278 , 7 . Google Scholar Crossref Search ADS WorldCat Wear , D. , Margileth , A., Hadfield , T., Fischer , G., Schlagel , C. & King , F. ( 1983 ) Cat scratch disease: a bacterial infection . Science. 221 , 1403 . Google Scholar Crossref Search ADS PubMed WorldCat This content is only available as a PDF. This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model) http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Clinical & Experimental Immunology Oxford University Press

Differential expression of the calcium-binding proteins MRP8 and MRP14 in granulomatous conditions: an immunohistochemical study

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References (26)

Publisher
Oxford University Press
Copyright
Copyright © 2022 British Society for Immunology
ISSN
0009-9104
eISSN
1365-2249
DOI
10.1111/j.1365-2249.1990.tb05301.x
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Abstract

SUMMARY MRP14 and MRP8 are well-characterized calcium-binding proteins present in myeloid cells and mononuclear phagocytes. These antigens can easily be visualized in paraffin-embedded tissue, making use of monospecific polyclonal antibodies. This study evaluates MRP14 and MRP8 expression in mononuclear phagocytes in various granulomatous conditions. MRP14 is strongly expressed in all granulomatous conditions. MRP8 is variably expressed. Mononuclear phagocytes in granulomas of foreign body type, cat-scratch discase and erythema nodosum strongly express MRP8. In contrast, MRP8 expression is weak or absent in mononuclear phagocytes of sarcoidosis and tuberculosis. These results show differences in immunophenotype between non-phagocytic mononuclear phagocytes in delayed hypersensitivity type granulomas and phagocytic mononuclear phagocytes in non-hypersensitivity and non-immunological granulomas. granuloma, immunohistochemistry, delayed hypersensitivity REFERENCES Adams , T.E. , Bodmer , J.G. & Boomer , W.F. ( 1983 ) Production and characterization of monoclonal antibodies recognizing the chain subunits of human Ia allogens . Immunology , 50 , 613 . Google Scholar PubMed OpenURL Placeholder Text WorldCat Branutzaeg , P. , Dale , I. Fagerhol , M. ( 1987 ) Distribution of a formalin-resistant myelomonocytic antigen in human tissues. I. Comparison with other leucocyte markers by paired immunofluorescence and immunoenzyme staining . Am. J. clin. Pathol. 87 , 681 . Google Scholar Crossref Search ADS PubMed WorldCat Dale , I. , Brandtzaeg , P., Fagerhol , M.K. & Scott , H. ( 1985 ) Distribution of a new myelomonocytic antigen (L1) in human peripheral blood leukocytes. Immunofluorescence and immunoperoxidase staining features in comparison with lysozyme and lactoferrin . Am. J. clin. Pathol. 84 , 24 . Google Scholar Crossref Search ADS PubMed WorldCat Epstein , W.L. ( 1980 ) Foreign body granulomas . In Basic and Clinical Aspects of Granulomatous Diseases (ed. by D. L. Horns & T. Yoshida) p. 181 . Elsevier , Holland . Google Scholar Google Preview OpenURL Placeholder Text WorldCat COPAC Flavell , D. , Jones , D. & Wright , D. ( 1987 ) Identification of tissue histiocytes on paraffin sections by a new monoclonal antibody . J. Histochem. Cytochem. 35 , 1217 . Google Scholar Crossref Search ADS PubMed WorldCat Hsu , S.M. , Raine , L. & Fanger , H. ( 1981 ) The use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase technique: a comparison between ABC and unlabelled antibody (PAP) procedures . J. Histochem. Cytochem. 29 , 577 . Google Scholar Crossref Search ADS PubMed WorldCat Kaufmann , S. & Flesch , I. ( 1988 ) The role of T cell macrophage interactions in tuberculosis . Springer Semin. Immunopathol. 10 , 337 . Google Scholar Crossref Search ADS PubMed WorldCat Mlnro , C.S. , Campbell , D.A., Collings , L.A. & Poulter , L.W. ( 1987 ) Monoclonal antibodies distinguish macrophages and epitheliod cells in sarcoidosis and leprosy . Clin. exp. Immunol. 68 , 282 . Google Scholar PubMed OpenURL Placeholder Text WorldCat Niemi , K.M. , Förström , L., Hannuksela , M. & Mlstakallio , K.K. ( 1977 ) Nodules on the legs . Acta dermatovenerol. 57 , 145 . Google Scholar OpenURL Placeholder Text WorldCat Odink , K. , Cerletti , N., Brüggen , J., Clerc , R.G., Tarcsay , L., Zwadlo , G., Gerhards , G., Schlegel , R. & Sorg , C. ( 1987 ) Two calcium-binding proteins in infiltrate macrophages of rheumatoid arthritis . Nature. 330 , 80 . Google Scholar Crossref Search ADS PubMed WorldCat Poulter , L. , Campbell , D., Munro , C. & Janossy , G. ( 1986 ) Discrimination of human macrophages and dendritic cells using monoclonal antibodies . Scand. J. Immunol. 24 , 351 . Google Scholar Crossref Search ADS PubMed WorldCat Pulford , K. , Rlgney , E., Jones , M., Stross , P., Micklem , K., Gatter , K. & Mason , D.Y. ( 1989 ) KP1—a new monoclonal antibody detecting a monocyte/macrophage associated antigen in routinely processed tissue sections . J. clin. Palhol. 42 , 414 . Google Scholar Crossref Search ADS WorldCat Seal , R. , Edwards , J. & Hayes , M. ( 1975 ) The response of the lung to inhaled antigens . Prog. respir. Res. 8 , 125 . Google Scholar Crossref Search ADS WorldCat Semenzato , G. ( 1988 ) Recent advances on sarcoid immunology . Sarcoidosis. 5 , 5 . Google Scholar PubMed OpenURL Placeholder Text WorldCat Spector , W.G. & Heesom , N. ( 1969 ) The production of granulomata by antigen antibody complexes . J. Pathol. 98 , 31 . Google Scholar Crossref Search ADS PubMed WorldCat van den Oord , J.J. , de Wolf-Peeters , C., Facchetti , F. & Desmet , V. J. ( 1984 ) Immunohistochemical analysis of tuberculous and sarcoidal lymphadentis . Hum. Pathol. 15 , 559 . Google Scholar Crossref Search ADS PubMed WorldCat Warren , K.S. ( 1976 ) A functional classification of granulomatous inflammation . Ann. NY Acad. Sci. 278 , 7 . Google Scholar Crossref Search ADS WorldCat Wear , D. , Margileth , A., Hadfield , T., Fischer , G., Schlagel , C. & King , F. ( 1983 ) Cat scratch disease: a bacterial infection . Science. 221 , 1403 . Google Scholar Crossref Search ADS PubMed WorldCat This content is only available as a PDF. This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)

Journal

Clinical & Experimental Immunology – Oxford University Press

Published: Jun 28, 2008

Keywords: granuloma; delayed hypersensitivity; calcium-binding proteins; reticuloendothelial system; tuberculosis; polyclonal antibody; erythema nodosum; foreign bodies; immunophenotyping; myeloid cells; paraffin; sarcoidosis; immunohistochemistry; hypersensitivity; cat-scratch disease; antigens

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