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The basis for the mechanistic bias for deletional over inversional V(D)J recombination.

The basis for the mechanistic bias for deletional over inversional V(D)J recombination. Downloaded from genesdev.cshlp.org on October 31, 2021 - Published by Cold Spring Harbor Laboratory Press The basis for the mechanistic bias for deletional over inversional V(D)J recombination George H. Gauss and Michael R. Lieber 1 Laboratory of Experimental Oncology, Department of Pathology, Stanford University School of Medicine, Stanford, California 94305-5324 USA V(D)J recombination between recognition sites in the genome is characterized by certain biases. At some loci, proximal sites undergo recombination substantially more frequently than distal ones. The joining of DH/JH is an example of this. Because the D H element bears signal sequences on each side, inversion would be expected as often as deletion in DH/JH recombination. However, the markedly favored outcome is deletion, entailing utilization of the closer recombination site. One model proposed to explain these biases is the tracking model in which the recombinase tracks from one site to the other. Here, we have directly tested for various types of tracking in V(D)J recombination and have found no indication that it occurs. In addition, we have created DI~--JH minilocus substrates for analysis of the basis for the preference for deletion. We find that we can reproduce the deletional bias for the system. Moreover, by flipping the http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Genes & Development Unpaywall

The basis for the mechanistic bias for deletional over inversional V(D)J recombination.

Gauss, G H; Lieber, M R
Genes & Development – Aug 1, 1992
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Unpaywall
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0890-9369
DOI
10.1101/gad.6.8.1553
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Abstract

Downloaded from genesdev.cshlp.org on October 31, 2021 - Published by Cold Spring Harbor Laboratory Press The basis for the mechanistic bias for deletional over inversional V(D)J recombination George H. Gauss and Michael R. Lieber 1 Laboratory of Experimental Oncology, Department of Pathology, Stanford University School of Medicine, Stanford, California 94305-5324 USA V(D)J recombination between recognition sites in the genome is characterized by certain biases. At some loci, proximal sites undergo recombination substantially more frequently than distal ones. The joining of DH/JH is an example of this. Because the D H element bears signal sequences on each side, inversion would be expected as often as deletion in DH/JH recombination. However, the markedly favored outcome is deletion, entailing utilization of the closer recombination site. One model proposed to explain these biases is the tracking model in which the recombinase tracks from one site to the other. Here, we have directly tested for various types of tracking in V(D)J recombination and have found no indication that it occurs. In addition, we have created DI~--JH minilocus substrates for analysis of the basis for the preference for deletion. We find that we can reproduce the deletional bias for the system. Moreover, by flipping the

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Genes & Development – Unpaywall

Published: Aug 1, 1992

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