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Elongation of the<i>Kcnq1ot1</i>transcript is required for genomic imprinting of neighboring genes

Elongation of theKcnq1ot1transcript is required for genomic imprinting of neighboring genes Downloaded from genesdev.cshlp.org on November 4, 2021 - Published by Cold Spring Harbor Laboratory Press Elongation of the Kcnq1ot1 transcript is required for genomic imprinting of neighboring genes Debora Mancini-DiNardo, Scott J.S. Steele, John M. Levorse, Robert S. Ingram, and Shirley M. Tilghman Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA The imprinted gene cluster at the telomeric end of mouse chromosome 7 contains a differentially methylated CpG island, KvDMR, that is required for the imprinting of multiple genes, including the genes encoding the maternally expressed placental-specific transcription factor ASCL2, the cyclin-dependent kinase CDKN1C, and the potassium channel KCNQ1. The KvDMR, which maps within intron 10 of Kcnq1, contains the promoter for a paternally expressed, noncoding, antisense transcript, Kcnq1ot1. A 244-base-pair deletion of the promoter on the paternal allele leads to the derepression of all silent genes tested. To distinguish between the loss of silencing as the consequence of the absence of transcription or the transcript itself, we prematurely truncated the Kcnq1ot1 transcript by inserting a transcriptional stop signal downstream of the promoter. We show that the lack of a full-length Kcnq1ot1 transcript on the paternal chromosome leads to the expression of genes that are normally http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Genes & Development Unpaywall

Elongation of the<i>Kcnq1ot1</i>transcript is required for genomic imprinting of neighboring genes

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Unpaywall
ISSN
0890-9369
DOI
10.1101/gad.1416906
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Abstract

Downloaded from genesdev.cshlp.org on November 4, 2021 - Published by Cold Spring Harbor Laboratory Press Elongation of the Kcnq1ot1 transcript is required for genomic imprinting of neighboring genes Debora Mancini-DiNardo, Scott J.S. Steele, John M. Levorse, Robert S. Ingram, and Shirley M. Tilghman Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA The imprinted gene cluster at the telomeric end of mouse chromosome 7 contains a differentially methylated CpG island, KvDMR, that is required for the imprinting of multiple genes, including the genes encoding the maternally expressed placental-specific transcription factor ASCL2, the cyclin-dependent kinase CDKN1C, and the potassium channel KCNQ1. The KvDMR, which maps within intron 10 of Kcnq1, contains the promoter for a paternally expressed, noncoding, antisense transcript, Kcnq1ot1. A 244-base-pair deletion of the promoter on the paternal allele leads to the derepression of all silent genes tested. To distinguish between the loss of silencing as the consequence of the absence of transcription or the transcript itself, we prematurely truncated the Kcnq1ot1 transcript by inserting a transcriptional stop signal downstream of the promoter. We show that the lack of a full-length Kcnq1ot1 transcript on the paternal chromosome leads to the expression of genes that are normally

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Genes & Development – Unpaywall

Published: May 15, 2006

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