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Functional characterization of human SLC41A1, a Mg2+ transporter with similarity to prokaryotic MgtE Mg2+ transporters

Functional characterization of human SLC41A1, a Mg2+ transporter with similarity to prokaryotic... We have begun to identify and characterize genes that are differentially expressed with low magnesium. One of these sequences conformed to the solute carrier SLC41A1. Real-time RT-PCR of RNA isolated from renal distal tubule epithelial mouse distal convoluted tubule (MDCT) cells cultured in low-magnesium media relative to normal media and in the kidney cortex of mice maintained on low-magnesium diets compared with those animals consuming normal diets confirmed that the SLC41A1 transcript is responsive to magnesium. Mouse SLC41A1 was cloned from MDCT cells, expressed in Xenopus laevis oocytes, and studied with two-electrode voltage-clamp studies. When expressed in oocytes, SLC41A1 mediates saturable Mg 2+ uptake with a Michaelis constant of 0.67 mM. Transport of Mg 2+ by SLC41A1 is rheogenic, voltage dependent, and not coupled to Na + or Cl – . Expressed SLC41A1 transports a range of other divalent cations: Mg 2+ , Sr 2+ , Zn 2+ , Cu 2+ , Fe 2+ , Co 2+ , Ba 2+ , and Cd 2+ . The divalent cations Ca 2+ , Mn 2+ , and Ni 2+ and the trivalent ion Gd 3+ did not induce currents nor did they inhibit Mg 2+ transport. The nonselective cation La 3+ abolished Mg 2+ uptake. The SLC41A1 transcript is present in many tissues, notably renal epithelial cells, and is upregulated in some tissues with magnesium deficiency. These studies suggest that SLC41A1 is a regulated Mg 2+ transporter that might be involved in magnesium homeostasis in epithelial cells. magnesium; differentially regulated; expression; Xenopus oocytes http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Physiological Genomics The American Physiological Society

Functional characterization of human SLC41A1, a Mg2+ transporter with similarity to prokaryotic MgtE Mg2+ transporters

Goytain, Angela; Quamme, Gary A.
Physiological Genomics , Volume 21 (3): 337 – May 11, 2005
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References (19)

Publisher
The American Physiological Society
Copyright
Copyright © 2011 the American Physiological Society
ISSN
1094-8341
eISSN
1531-2267
DOI
10.1152/physiolgenomics.00261.2004
pmid
15713785
Publisher site
See Article on Publisher Site

Abstract

We have begun to identify and characterize genes that are differentially expressed with low magnesium. One of these sequences conformed to the solute carrier SLC41A1. Real-time RT-PCR of RNA isolated from renal distal tubule epithelial mouse distal convoluted tubule (MDCT) cells cultured in low-magnesium media relative to normal media and in the kidney cortex of mice maintained on low-magnesium diets compared with those animals consuming normal diets confirmed that the SLC41A1 transcript is responsive to magnesium. Mouse SLC41A1 was cloned from MDCT cells, expressed in Xenopus laevis oocytes, and studied with two-electrode voltage-clamp studies. When expressed in oocytes, SLC41A1 mediates saturable Mg 2+ uptake with a Michaelis constant of 0.67 mM. Transport of Mg 2+ by SLC41A1 is rheogenic, voltage dependent, and not coupled to Na + or Cl – . Expressed SLC41A1 transports a range of other divalent cations: Mg 2+ , Sr 2+ , Zn 2+ , Cu 2+ , Fe 2+ , Co 2+ , Ba 2+ , and Cd 2+ . The divalent cations Ca 2+ , Mn 2+ , and Ni 2+ and the trivalent ion Gd 3+ did not induce currents nor did they inhibit Mg 2+ transport. The nonselective cation La 3+ abolished Mg 2+ uptake. The SLC41A1 transcript is present in many tissues, notably renal epithelial cells, and is upregulated in some tissues with magnesium deficiency. These studies suggest that SLC41A1 is a regulated Mg 2+ transporter that might be involved in magnesium homeostasis in epithelial cells. magnesium; differentially regulated; expression; Xenopus oocytes

Journal

Physiological Genomics – The American Physiological Society

Published: May 11, 2005

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