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Development of naked growing mouse oocytes in vitro

Development of naked growing mouse oocytes in vitro Half grown oocytes were released from follicles of 7‐day‐old mouse ovaries during overnight culture. Naked oocytes free of attached follicular cells were cultured in MEM plus 10% fetal calf serum over the developing monolayer of ovarian cells. Oocytes increased in diameter for a period of at least a week at 1.6 μ per day, or 70% of the in vivo growth rate. The ultrastructure of cytoplasmic organelles in cultured growing oocytes was normal. During the second week, about 35% underwent spontaneous fragmentation, and at least 10–20% resumed meiotic maturation. We conclude that a significant fraction of naked oocytes reached a functional state comparable to that of normal full grown oocytes at a rate approaching the in vivo rate. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Experimental Zoology Part A: Ecological and Integrative Physiology Wiley

Development of naked growing mouse oocytes in vitro

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References (35)

Publisher
Wiley
Copyright
Copyright © 1980 Wiley Subscription Services, Inc., A Wiley Company
ISSN
2471-5638
eISSN
2471-5646
DOI
10.1002/jez.1402110206
pmid
6989948
Publisher site
See Article on Publisher Site

Abstract

Half grown oocytes were released from follicles of 7‐day‐old mouse ovaries during overnight culture. Naked oocytes free of attached follicular cells were cultured in MEM plus 10% fetal calf serum over the developing monolayer of ovarian cells. Oocytes increased in diameter for a period of at least a week at 1.6 μ per day, or 70% of the in vivo growth rate. The ultrastructure of cytoplasmic organelles in cultured growing oocytes was normal. During the second week, about 35% underwent spontaneous fragmentation, and at least 10–20% resumed meiotic maturation. We conclude that a significant fraction of naked oocytes reached a functional state comparable to that of normal full grown oocytes at a rate approaching the in vivo rate.

Journal

The Journal of Experimental Zoology Part A: Ecological and Integrative Physiology – Wiley

Published: Feb 1, 1980

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