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Immune reconstitution after transplantation of autologous peripheral CD34+ cells: analysis of predictive factors and comparison with unselected progenitor transplants

Immune reconstitution after transplantation of autologous peripheral CD34+ cells: analysis of... The recovery of lymphocyte count, CD4+ and CD8+ T‐cell subsets, natural killer (NK) cells and CD19+ B‐cells was evaluated in a cohort of 15 patients receiving autologous CD34+ peripheral blood progenitor cells (PBPCs; group A) for haematological malignancies and in 20 patients transplanted with autologous unselected PBPCs (group B). Lymphocyte count recovered in both patient cohorts, being significantly lower in group A than in group B 1 (P = 0·008) and 2 months (P = 0·0035) after progenitor cell infusion. The repopulation of CD3+ T‐cells occurred more rapidly in group B than in group A (P = 0·034 on week 4); CD19+ B‐lymphocytes did not return to reference ranges in either group of patients. The count of CD4+ T‐lymphocytes remained < 200/μl during the study period in patients transplanted with CD34+ PBPCs, significantly lower than group B levels (P = 0·034 and P = 0·021 on weeks 4 and 8 respectively). CD8+ T‐cells increased rapidly in both groups; thus, the CD4 to CD8 ratio was severely reduced. CD4+ and CD8+ T‐cells displayed an activated phenotype in both groups of patients, co‐expressing the HLA‐DR antigen throughout the study period. NK cells followed a similar repopulation kinetics in both study groups, although their expansion was greater in group B than in group A (P = 0·014 on week 4). In the CD34+ group, post‐transplant administration of granulocyte colony‐stimulating factor predicted a faster lymphocyte recovery in multivariate analysis (P = 0·025); interestingly, the amount of passively transferred lymphocytes correlated inversely with time to achieve a lymphocyte count > 0·5 × 109/l (r = –0·63, P = 0·01). Further investigations are necessary to characterize T‐cell competence after transplantation of CD34+ PBPCs. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png British Journal of Haematology Wiley

Immune reconstitution after transplantation of autologous peripheral CD34+ cells: analysis of predictive factors and comparison with unselected progenitor transplants

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References (37)

Publisher
Wiley
Copyright
Copyright © 2000 Wiley Subscription Services
ISSN
0007-1048
eISSN
1365-2141
DOI
10.1046/j.1365-2141.2000.01824.x
Publisher site
See Article on Publisher Site

Abstract

The recovery of lymphocyte count, CD4+ and CD8+ T‐cell subsets, natural killer (NK) cells and CD19+ B‐cells was evaluated in a cohort of 15 patients receiving autologous CD34+ peripheral blood progenitor cells (PBPCs; group A) for haematological malignancies and in 20 patients transplanted with autologous unselected PBPCs (group B). Lymphocyte count recovered in both patient cohorts, being significantly lower in group A than in group B 1 (P = 0·008) and 2 months (P = 0·0035) after progenitor cell infusion. The repopulation of CD3+ T‐cells occurred more rapidly in group B than in group A (P = 0·034 on week 4); CD19+ B‐lymphocytes did not return to reference ranges in either group of patients. The count of CD4+ T‐lymphocytes remained < 200/μl during the study period in patients transplanted with CD34+ PBPCs, significantly lower than group B levels (P = 0·034 and P = 0·021 on weeks 4 and 8 respectively). CD8+ T‐cells increased rapidly in both groups; thus, the CD4 to CD8 ratio was severely reduced. CD4+ and CD8+ T‐cells displayed an activated phenotype in both groups of patients, co‐expressing the HLA‐DR antigen throughout the study period. NK cells followed a similar repopulation kinetics in both study groups, although their expansion was greater in group B than in group A (P = 0·014 on week 4). In the CD34+ group, post‐transplant administration of granulocyte colony‐stimulating factor predicted a faster lymphocyte recovery in multivariate analysis (P = 0·025); interestingly, the amount of passively transferred lymphocytes correlated inversely with time to achieve a lymphocyte count > 0·5 × 109/l (r = –0·63, P = 0·01). Further investigations are necessary to characterize T‐cell competence after transplantation of CD34+ PBPCs.

Journal

British Journal of Haematology – Wiley

Published: Jan 1, 2000

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