Access the full text.
Sign up today, get DeepDyve free for 14 days.
Loading next page...
/lp/springer-journals/in-gel-digestion-for-mass-spectrometric-characterization-of-proteins-fUhevr01ET
References (23)
-
R. Aebersold, M. Mann (2003)
Mass spectrometry-based proteomicsNature, 422
-
Lyris Godoy, J. Olsen, G. Souza, Guoqing Li, P. Mortensen, M. Mann (2006)
Status of complete proteome analysis by mass spectrometry: SILAC labeled yeast as a model systemGenome Biology, 7
-
G. Mitulovič, M. Smoluch, J. Chervet, Ines Steinmacher, A. Kungl, K. Mechtler (2003)
An improved method for tracking and reducing the void volume in nano HPLC–MS with micro trapping columnsAnalytical and Bioanalytical Chemistry, 376
-
C. Mason, Terry Therneau, Jeanette Eckel-Passow, Kenneth Johnson, Ann Oberg, Janet Olson, K. Nair, D. Muddiman, H. Bergen (2007)
A Method for Automatically Interpreting Mass Spectra of 18O-Labeled Isotopic Clusters*SMolecular & Cellular Proteomics, 6
-
A. Pichler, P. Knipscheer, E. Oberhofer, W. Dijk, R. Körner, J. Olsen, S. Jentsch, F. Melchior, T. Sixma (2005)
SUMO modification of the ubiquitin-conjugating enzyme E2-25KNature Structural &Molecular Biology, 12
-
N. Wielsch, Henrik Thomas, V. Surendranath, P. Waridel, Ari Frank, P. Pevzner, A. Shevchenko (2006)
Rapid validation of protein identifications with the borderline statistical confidence via de novo sequencing and MS BLAST searches.Journal of proteome research, 5 9
-
A. Shevchenko, A. Shevchenko (2001)
Evaluation of the efficiency of in-gel digestion of proteins by peptide isotopic labeling and MALDI mass spectrometry.Analytical biochemistry, 296 2
-
A. Shevchenko, I. Chernushevich, W. Ens, K. Standing, B. Thomson, M. Wilm, M. Mann (1997)
Rapid 'de novo' peptide sequencing by a combination of nanoelectrospray, isotopic labeling and a quadrupole/time-of-flight mass spectrometer.Rapid communications in mass spectrometry : RCM, 11 9
-
A. Shevchenko, M. Wilm, O. Vorm, Millard Mann (1996)
Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels.Analytical chemistry, 68 5
-
J. Rappsilber, Y. Ishihama, M. Mann (2003)
Stop and go extraction tips for matrix-assisted laser desorption/ionization, nanoelectrospray, and LC/MS sample pretreatment in proteomics.Analytical chemistry, 75 3
-
H. Steen, M. Mann (2004)
The abc's (and xyz's) of peptide sequencingNature Reviews Molecular Cell Biology, 5
-
A. Shevchenko, S. Sunyaev, A. Liska, P. Bork, A. Shevchenko (2003)
Nanoelectrospray tandem mass spectrometry and sequence similarity searching for identification of proteins from organisms with unknown genomes.Methods in molecular biology, 211
-
J. Havliš, A. Shevchenko (2004)
Absolute quantification of proteins in solutions and in polyacrylamide gels by mass spectrometry.Analytical chemistry, 76 11
-
Shao-En Ong, B. Blagoev, I. Kratchmarova, D. Kristensen, H. Steen, A. Pandey, M. Mann (2002)
Stable Isotope Labeling by Amino Acids in Cell Culture, SILAC, as a Simple and Accurate Approach to Expression Proteomics*Molecular & Cellular Proteomics, 1
-
Xudong Yao, Amy Freas, J. Ramirez, P. Demirev, C. Fenselau (2001)
Proteolytic 18O labeling for comparative proteomics: model studies with two serotypes of adenovirus.Analytical chemistry, 73 13
-
Shao-En Ong, M. Mann (2005)
Mass spectrometry–based proteomics turns quantitativeNature Chemical Biology, 1
-
Henrik Thomas, J. Havliš, J. Peychl, A. Shevchenko (2004)
Dried-droplet probe preparation on AnchorChip targets for navigating the acquisition of matrix-assisted laser desorption/ionization time-of-flight spectra by fluorescence of matrix/analyte crystals.Rapid communications in mass spectrometry : RCM, 18 9
-
Shao-En Ong, M. Mann (2006)
A practical recipe for stable isotope labeling by amino acids in cell culture (SILAC)Nature Protocols, 1
-
M. Šebela, T. Stosová, J. Havliš, N. Wielsch, Henrik Thomas, Z. Zdráhal, A. Shevchenko (2006)
Thermostable trypsin conjugates for high‐throughput proteomics: synthesis and performance evaluationPROTEOMICS, 6
-
A. Shevchenko, A. Loboda, W. Ens, B. Schraven, K. Standing, A. Shevchenko (2001)
Archived polyacrylamide gels as a resource for proteome characterization by mass spectrometryELECTROPHORESIS, 22
-
J. Havliš, Henrik Thomas, M. Šebela, A. Shevchenko (2003)
Fast-response proteomics by accelerated in-gel digestion of proteins.Analytical chemistry, 75 6
-
J. Olsen, M. Mann (2004)
Improved peptide identification in proteomics by two consecutive stages of mass spectrometric fragmentation.Proceedings of the National Academy of Sciences of the United States of America, 101 37
-
A. Shevchenko, I. Chernushevich, M. Wilm, M. Mann (2000)
De Novo peptide sequencing by nanoelectrospray tandem mass spectrometry using triple quadrupole and quadrupole/time-of-flight instruments.Methods in molecular biology, 146
- Publisher
- Springer Journals
- Copyright
- Copyright © 2007 by Nature Publishing Group
- Subject
- Life Sciences; Life Sciences, general; Biological Techniques; Analytical Chemistry; Microarrays; Computational Biology/Bioinformatics; Organic Chemistry
- ISSN
- 1754-2189
- eISSN
- 1750-2799
- DOI
- 10.1038/nprot.2006.468
- Publisher site
- See Article on Publisher Site
Abstract
In-gel digestion of proteins isolated by gel electrophoresis is a cornerstone of mass spectrometry (MS)-driven proteomics. The 10-year-old recipe by Shevchenko et al. has been optimized to increase the speed and sensitivity of analysis. The protocol is for the in-gel digestion of both silver and Coomassie-stained protein spots or bands and can be followed by MALDI-MS or LC-MS/MS analysis to identify proteins at sensitivities better than a few femtomoles of protein starting material.
Journal
Nature Protocols – Springer Journals
Published: Jan 25, 2007