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2-D Proteome Analysis ProtocolsProtein Identification and Analysis Tools in the ExPASy Server

2-D Proteome Analysis Protocols: Protein Identification and Analysis Tools in the ExPASy Server [Protein identification and analysis software performs a central role in the investigation of proteins from two-dimensional (2-D) gels. For protein identification, the user matches certain empirically gained information against a protein database to define a protein as already known or as novel. For protein analysis, information in protein databases can be used to predict certain properties about a protein, which can be useful for its empirical investigation. The two processes are thus complementary. Although there are numerable programs available for the above-mentioned applications, we have developed a set of original tools with a few main goals in mind. Specifically, these are: To utilize the extensive annotation available in the SWISS-PROT database (1) wherever possible.To have tools specifically, but not exclusively, applicable to proteins prepared by 2-D gel electrophoresis.To have all tools available on the World Wide Web and freely available to the scientific community.] http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

2-D Proteome Analysis ProtocolsProtein Identification and Analysis Tools in the ExPASy Server

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References (35)

Publisher
Humana Press
Copyright
© Humana Press 1999
ISBN
978-0-89603-524-9
Pages
531–552
DOI
10.1385/1-59259-584-7:531
Publisher site
See Chapter on Publisher Site

Abstract

[Protein identification and analysis software performs a central role in the investigation of proteins from two-dimensional (2-D) gels. For protein identification, the user matches certain empirically gained information against a protein database to define a protein as already known or as novel. For protein analysis, information in protein databases can be used to predict certain properties about a protein, which can be useful for its empirical investigation. The two processes are thus complementary. Although there are numerable programs available for the above-mentioned applications, we have developed a set of original tools with a few main goals in mind. Specifically, these are: To utilize the extensive annotation available in the SWISS-PROT database (1) wherever possible.To have tools specifically, but not exclusively, applicable to proteins prepared by 2-D gel electrophoresis.To have all tools available on the World Wide Web and freely available to the scientific community.]

Published: Jan 1, 1999

Keywords: Amino Acid Composition; Query Protein; Database Entry; ExPASy Server; Calibration Protein

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