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The effect of protein and urine on uropathogen adhesion to polymer substrata

The effect of protein and urine on uropathogen adhesion to polymer substrata Uropathogenic adhesion was measured using a range of polymer materials with differing surface tension properties. Experiments were carried out in the presence of phosphate buffered saline (controls), Tamm Horsfall protein (THP), and human urine with quantitation by image analysis. The results showed that THP did not bind to the polymer materials and therefore did not act as a receptor surface for type 1 fimbriated Escherichia coli. However, the THP did interfere with adhesion by binding directly to these organisms as well as to Pseudomonas aeruginosa, Staphylococcus epidermidis, and, to a lesser extent, Proteus mirabilis. Incubation of the uropathogens in THP and urine resulted in altered adhesion profiles to polymer surfaces, with no single trend apparent. The results emphasize that fluid components, particularly proteins, and substratum surface tension influence bacterial adhesion to biomaterials. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Biomedical Materials Research Part A Wiley

The effect of protein and urine on uropathogen adhesion to polymer substrata

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References (22)

Publisher
Wiley
Copyright
Copyright © 1990 Wiley Subscription Services, Inc., A Wiley Company
ISSN
1549-3296
eISSN
1552-4965
DOI
10.1002/jbm.820241005
pmid
2283351
Publisher site
See Article on Publisher Site

Abstract

Uropathogenic adhesion was measured using a range of polymer materials with differing surface tension properties. Experiments were carried out in the presence of phosphate buffered saline (controls), Tamm Horsfall protein (THP), and human urine with quantitation by image analysis. The results showed that THP did not bind to the polymer materials and therefore did not act as a receptor surface for type 1 fimbriated Escherichia coli. However, the THP did interfere with adhesion by binding directly to these organisms as well as to Pseudomonas aeruginosa, Staphylococcus epidermidis, and, to a lesser extent, Proteus mirabilis. Incubation of the uropathogens in THP and urine resulted in altered adhesion profiles to polymer surfaces, with no single trend apparent. The results emphasize that fluid components, particularly proteins, and substratum surface tension influence bacterial adhesion to biomaterials.

Journal

Journal of Biomedical Materials Research Part A – Wiley

Published: Oct 1, 1990

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