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- Copyright
- © 2007 Society for Leukocyte Biology
- eISSN
- 1938-3673
- DOI
- 10.1189/jlb.1306200
- Publisher site
- See Article on Publisher Site
Abstract
Interview with Dr. Heikki Rauvala regarding Pivotal Advance: Analysis of proinflammatory activity of highly purified eukaryotic recombinant HMGB1 (amphoterin) Marco E. Bianchi San Raffaele University, Chromatin Dynamics Unit, Milan, Italy This work was selected as a Pivotal Advance because the paper shows that HMGB1, also co-discovered as amphoterin, in a fully purified state does not activate Toll-like receptors 2 and 4, but nevertheless still has considerable DNA-binding capac- ity, chemotactic effects, and developmental activities. Dr. Rauvala, you were the first to purify an extracellular protein that, as it turned out, is also a nuclear protein. How did you come across amphoterin? HR: In the mid 80s we were searching for neurite outgrowth promoting factors that would act on cortical neurons. This had been my major research goal for several years, since I had established my own lab after a postdoctoral experience in the US in 1979 –1980. Not many such factors were known, and the most interesting were fibroblast growth factors (FGFs). They were shown to regulate neuronal development, and to bind strongly to heparin and heparan sulfates. We homogenized young rat brains, and fractionated extracts on a heparin col- umn, where not many proteins would bind at high
Journal
Journal of Leukocyte Biology – Oxford University Press
Published: Oct 12, 2006