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Bone Morphogenetic Protein 2 Induces Dental Follicle Cells to Differentiate Toward a Cementoblast/Osteoblast Phenotype*

Bone Morphogenetic Protein 2 Induces Dental Follicle Cells to Differentiate Toward a... When triggered appropriately, dental follicle cells are considered to be able to differentiate toward a cementoblast/osteoblast phenotype. However, factors and mechanisms regulating follicle cell differentiation remain undefined. This study focused on determining the ability of bone morphogenetic protein (BMP) 2 to promote the differentiation of follicle cells and periodontal ligament (PDL) cells along a cementoblast/osteoblast pathway. Follicle cells and PDL cells were isolated from the first molar region of CD‐1 mice and immortalized with SV40. Both cell types expressed BMP‐4 and BMP receptors (BMPR) IA and II, but only follicle cells expressed BMP‐2 mRNA. Cells were exposed to recombinant human BMP (rhBMP)‐2 (0–100 ng/ml) and Northern blots were used to determine the expression of mineral‐associated markers. BMP‐2, in a dose‐ and time‐dependent manner, induced cementoblast/osteoblast differentiation of follicle cells, as reflected by enhanced core binding factor α1 (Cbfa1), bone sialoprotein (BSP), and osteocalcin (OCN) mRNA expression and enhanced mineral formation. U0126, a specific inhibitor of MEK‐1/2 members of the MAPK family, abolished BMP‐2‐mediated expression of BSP and OCN. In contrast, exposure of PDL cells to BMP‐2 resulted in modest expression of OCN and minimal promotion of mineralization. These results suggest that BMP‐2 triggers follicle cells to differentiate toward a cementoblast/osteoblast phenotype and that the MAPK pathway is involved. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Bone and Mineral Research Oxford University Press

Bone Morphogenetic Protein 2 Induces Dental Follicle Cells to Differentiate Toward a Cementoblast/Osteoblast Phenotype*

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References (76)

Publisher
Oxford University Press
Copyright
Copyright © 2002 ASBMR
ISSN
0884-0431
eISSN
1523-4681
DOI
10.1359/jbmr.2002.17.8.1441
Publisher site
See Article on Publisher Site

Abstract

When triggered appropriately, dental follicle cells are considered to be able to differentiate toward a cementoblast/osteoblast phenotype. However, factors and mechanisms regulating follicle cell differentiation remain undefined. This study focused on determining the ability of bone morphogenetic protein (BMP) 2 to promote the differentiation of follicle cells and periodontal ligament (PDL) cells along a cementoblast/osteoblast pathway. Follicle cells and PDL cells were isolated from the first molar region of CD‐1 mice and immortalized with SV40. Both cell types expressed BMP‐4 and BMP receptors (BMPR) IA and II, but only follicle cells expressed BMP‐2 mRNA. Cells were exposed to recombinant human BMP (rhBMP)‐2 (0–100 ng/ml) and Northern blots were used to determine the expression of mineral‐associated markers. BMP‐2, in a dose‐ and time‐dependent manner, induced cementoblast/osteoblast differentiation of follicle cells, as reflected by enhanced core binding factor α1 (Cbfa1), bone sialoprotein (BSP), and osteocalcin (OCN) mRNA expression and enhanced mineral formation. U0126, a specific inhibitor of MEK‐1/2 members of the MAPK family, abolished BMP‐2‐mediated expression of BSP and OCN. In contrast, exposure of PDL cells to BMP‐2 resulted in modest expression of OCN and minimal promotion of mineralization. These results suggest that BMP‐2 triggers follicle cells to differentiate toward a cementoblast/osteoblast phenotype and that the MAPK pathway is involved.

Journal

Journal of Bone and Mineral Research – Oxford University Press

Published: Dec 2, 2009

Keywords: bone morphogenetic protein; dental follicle; periodontal ligament; osteoblast; MAPK

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