Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 7-Day Trial for You or Your Team.

Learn More →

Highly enantioselective HPLC separations using the covalently bonded macrocyclic antibiotic, ristocetin A, chiral stationary phase

Highly enantioselective HPLC separations using the covalently bonded macrocyclic antibiotic,... The macrocyclic glycopeptide, ristocetin A, was covalently bonded to a silica gel support and evaluated as a liquid chromatographic (LC) chiral stationary phase (CSP). Over 230 racemates were resolved in either the reversed‐phase mode, the normal‐phase mode, or the polar‐organic mode. The retention behavior and selectivity of this CSP were examined in each mode. Optimization of separations on this column is discussed. The ristocetin A CSP appeared to be complimentary to other glycopeptide CSPs (i.e., vancomycin and teicoplanin). Column stability was excellent. The CSP was not irreversibly altered when going from one mobile phase mode to another. Chirality 10:434–483, 1998. © 1998 Wiley‐Liss, Inc. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Chirality Wiley

Highly enantioselective HPLC separations using the covalently bonded macrocyclic antibiotic, ristocetin A, chiral stationary phase

Loading next page...
 
/lp/wiley/highly-enantioselective-hplc-separations-using-the-covalently-bonded-oYzEWkBMMa

References (28)

Publisher
Wiley
Copyright
Copyright © 1998 Wiley Subscription Services
ISSN
0899-0042
eISSN
1520-636X
DOI
10.1002/(SICI)1520-636X(1998)10:5<434::AID-CHIR10>3.0.CO;2-0
pmid
9691460
Publisher site
See Article on Publisher Site

Abstract

The macrocyclic glycopeptide, ristocetin A, was covalently bonded to a silica gel support and evaluated as a liquid chromatographic (LC) chiral stationary phase (CSP). Over 230 racemates were resolved in either the reversed‐phase mode, the normal‐phase mode, or the polar‐organic mode. The retention behavior and selectivity of this CSP were examined in each mode. Optimization of separations on this column is discussed. The ristocetin A CSP appeared to be complimentary to other glycopeptide CSPs (i.e., vancomycin and teicoplanin). Column stability was excellent. The CSP was not irreversibly altered when going from one mobile phase mode to another. Chirality 10:434–483, 1998. © 1998 Wiley‐Liss, Inc.

Journal

ChiralityWiley

Published: Jan 1, 1998

Keywords: ; ; ; ;

There are no references for this article.