Access the full text.
Sign up today, get DeepDyve free for 14 days.
Loading next page...
/lp/springer-journals/the-structural-basis-for-specificity-of-substrate-and-recruitment-olkXLoypi0
References (38)
-
V. Skamnaki, David Owen, M. Noble, E. Lowe, G. Lowe, N. Oikonomakos, Louise Johnson (1999)
Catalytic mechanism of phosphorylase kinase probed by mutational studies.Biochemistry, 38 44
-
Ying‐Nan Chen, S. Sharma, T. Ramsey, Li Jiang, Mary Martin, Kayla Baker, P. Adams, K. Bair, W. Kaelin (1999)
Selective killing of transformed cells by cyclin/cyclin-dependent kinase 2 antagonists.Proceedings of the National Academy of Sciences of the United States of America, 96 8
-
J. Chen, P. Saha, S. Kornbluth, B. Dynlacht, Anindya Dutta (1996)
Cyclin-binding motifs are essential for the function of p21CIP1Molecular and Cellular Biology, 16
-
Correspondence and requests for materials should be addressed to L.N.J. Coordinates have been deposited in Protein DataBank under accession number 1QMZ -
Hideki Kobayashi, Elspeth Stewart, Randy Poon, P. Jorg, Adamczewski, Julian Gannon, Tim Huntt (1992)
Identification of the domains in cyclin A required for binding to, and activation of, p34cdc2 and p32cdk2 protein kinase subunits.Molecular biology of the cell, 3 11
-
B. Kelly, K. Wolfe, J. Roberts (1998)
Identification of a substrate-targeting domain in cyclin E necessary for phosphorylation of the retinoblastoma protein.Proceedings of the National Academy of Sciences of the United States of America, 95 5
-
Songyang Zhou, S. Blechner, N. Hoagland, M. Hoekstra, H. Piwnica-Worms, L. Cantley (1994)
Use of an oriented peptide library to determine the optimal substrates of protein kinasesCurrent Biology, 4
-
H. Bondt, J. Rosenblatt, J. Jancarik, H. Jones, David Morgant, Sung-Hou Kim (1993)
Crystal structure of cyclin-dependent kinase 2Nature, 363
-
A. Russo, P. Jeffrey, N. Pavletich (1996)
Structural basis of cyclin-dependent kinase activation by phosphorylationNature Structural Biology, 3
-
(1998)
Results and Problems in Cell Differentiation (ed -
N. Brown, M. Noble, J. Endicott, E. Garman, Soichi Wakatsuki, Edward Mitchell, B. Rasmussen, Tim Hunt, Louise Johnson (1995)
The crystal structure of cyclin A.Structure, 3 11
-
B. Petersen, J. Lukas, C. Sørensen, J. Bartek, K. Helin (1999)
Phosphorylation of mammalian CDC6 by Cyclin A/CDK2 regulates its subcellular localizationThe EMBO Journal, 18
-
B. Canagarajah, A. Khokhlatchev, M. Cobb, E. Goldsmith (1997)
Activation Mechanism of the MAP Kinase ERK2 by Dual PhosphorylationCell, 90
-
BY Navaza (1994)
AMoRe: an automated package for molecular replacementActa Crystallographica Section A, 50
-
W. Hackmann (1993)
Tesla's sparks of imaginationNature, 363
-
H. Schreuder, H. Groendijk, J. Laan, R. Wierenga (1988)
THE TRANSFER OF PROTEIN CRYSTALS FROM THEIR ORIGINAL MOTHER LIQUOR TO A SOLUTION WITH A COMPLETELY DIFFERENT PRECIPITANTJournal of Applied Crystallography, 21
-
T. Jones, J. Zou, S. Cowan, M. Kjeldgaard, Serge HAGgGE (1991)
Improved methods for building protein models in electron density maps and the location of errors in these models.Acta crystallographica. Section A, Foundations of crystallography, 47 ( Pt 2)
-
Erich Nigg (1993)
Targets of cyclin-dependent protein kinases.Current opinion in cell biology, 5 2
-
A. Russo, P. Jeffrey, A. Patten, J. Massagué, N. Pavletich (1996)
Crystal structure of the p27Kip1 cyclin-dependent-kinase inibitor bound to the cyclin A–Cdk2 complexNature, 382
-
H. Higashi, I. Suzuki-Takahashi, Y. Taya, K. Segawa, S. Nishimura, M. Kitagawa (1995)
Differences in substrate specificity between Cdk2-cyclin A and Cdk2-cyclin E in vitro.Biochemical and biophysical research communications, 216 2
-
D. Morgan (1997)
Cyclin-dependent kinases: engines, clocks, and microprocessors.Annual review of cell and developmental biology, 13
-
Peter Adams, Xiaotong Li, William Sellers, Kayla Baker, X. Leng, J. Harper, Y. Taya, William Kaelin (1999)
Retinoblastoma Protein Contains a C-terminal Motif That Targets It for Phosphorylation by Cyclin-cdk ComplexesMolecular and Cellular Biology, 19
-
J. Holmes, M. Solomon (1996)
A Predictive Scale for Evaluating Cyclin-dependent Kinase SubstratesThe Journal of Biological Chemistry, 271
-
V. Lamzin, K. Wilson (1993)
Automated refinement of protein models.Acta crystallographica. Section D, Biological crystallography, 49 Pt 1
-
RJ Read (1986)
Improved coefficients for map calculation using partial structures with errorsActa Crystallogr. A, 42
-
B. Schulman, D. Lindstrom, E. Harlow (1998)
Substrate recruitment to cyclin-dependent kinase 2 by a multipurpose docking site on cyclin A.Proceedings of the National Academy of Sciences of the United States of America, 95 18
-
Peter Adams, W. Sellers, Sushil Sharma, WU ARTHURD., C. Nalin, W. Kaelin (1996)
Identification of a cyclin-cdk2 recognition motif present in substrates and p21-like cyclin-dependent kinase inhibitorsMolecular and Cellular Biology, 16
-
Liang Zhu, E. Harlow, B. Dynlacht (1995)
p107 uses a p21CIP1-related domain to bind cyclin/cdk2 and regulate interactions with E2F.Genes & development, 9 14
-
Pushkar Sharma, P. Steinbach, M. Sharma,, N. Amin, J. Barchi, H. Pant (1999)
Identification of Substrate Binding Site of Cyclin-dependent Kinase 5*The Journal of Biological Chemistry, 274
-
B. Sarcevic, R. Lilischkis, R. Sutherland (1997)
Differential Phosphorylation of T-47D Human Breast Cancer Cell Substrates by D1-, D3-, E-, and A-type Cyclin-CDK Complexes*The Journal of Biological Chemistry, 272
-
G. Murshudov, A. Vagin, E. Dodson (1997)
Refinement of macromolecular structures by the maximum-likelihood method.Acta crystallographica. Section D, Biological crystallography, 53 Pt 3
-
N. Brown, M. Noble, A. Lawrie, M. Morris, P. Tunnah, G. Divita, L. Johnson, J. Endicott (1999)
Effects of Phosphorylation of Threonine 160 on Cyclin-dependent Kinase 2 Structure and Activity*The Journal of Biological Chemistry, 274
-
T. Zarkowska, S. U, E. Harlow, S. Mittnacht (1997)
Monoclonal antibodies specific for underphosphorylated retinoblastoma protein identify a cell cycle regulated phosphorylation site targeted by CDKsOncogene, 14
-
R. Read (1986)
Improved Fourier Coefficients for Maps Using Phases from Partial Structures with ErrorsActa Crystallographica Section A, 42
-
B. Dynlacht, K. Moberg, J. Lees, E. Harlow, L. Zhu (1997)
Specific regulation of E2F family members by cyclin-dependent kinasesMolecular and Cellular Biology, 17
-
P. Jeffrey, A. Russo, K. Polyak, E. Gibbs, J. Hurwitz, J. Massagué, N. Pavletich (1995)
Mechanism of CDK activation revealed by the structure of a cyclinA-CDK2 complexNature, 376
-
E. Lowe, Madeline Noble, V. Skamnaki, N. Oikonomakos, D. Owen, L. Johnson (1997)
The crystal structure of a phosphorylase kinase peptide substrate complex: kinase substrate recognitionThe EMBO Journal, 16
-
M. Kitagawa, H. Higashi, H. Jung, I. Suzuki-Takahashi, M. Ikeda, K. Tamai, Jun Kato, K. Segawa, E. Yoshida, S. Nishimura, Yoichi, Taya (1996)
The consensus motif for phosphorylation by cyclin D1‐Cdk4 is different from that for phosphorylation by cyclin A/E‐Cdk2.The EMBO Journal, 15
- Publisher
- Springer Journals
- Copyright
- Copyright © 1999 by Macmillan Magazines Ltd.
- Subject
- Life Sciences; Life Sciences, general; Cell Biology; Cancer Research; Developmental Biology; Stem Cells
- ISSN
- 1465-7392
- eISSN
- 1476-4679
- DOI
- 10.1038/15674
- Publisher site
- See Article on Publisher Site
Abstract
Progression through the eukaryotic cell cycle is driven by the orderly activation of cyclin-dependent kinases (CDKs). For activity, CDKs require association with a cyclin and phosphorylation by a separate protein kinase at a conserved threonine residue (T160 in CDK2). Here we present the structure of a complex consisting of phosphorylated CDK2 and cyclin A together with an optimal peptide substrate, HHASPRK. This structure provides an explanation for the specificity of CDK2 towards the proline that follows the phosphorylatable serine of the substrate peptide, and the requirement for the basic residue in the P+3 position of the substrate. We also present the structure of phosphorylated CDK2 plus cyclin A3 in complex with residues 658–668 from the CDK2 substrate p107. These residues include the RXL motif required to target p107 to cyclins. This structure explains the specificity of the RXL motif for cyclins.
Journal
Nature Cell Biology – Springer Journals
Published: Oct 14, 1999