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Repressor domain and nuclear localization signal of the murine Hoxa‐11 protein are located in the homeodomain: no evidence for role of poly alanine stretches in transcriptional repression

Repressor domain and nuclear localization signal of the murine Hoxa‐11 protein are located in the... Hoxa‐11 is a member of the homeodomain class of transcription factors, which play important roles in metazoan development. Hoxa‐11 is particularly interesting because it is involved in a major mammalian innovation, uterus development and gestation. We are interested in the molecular changes underlying this evolutionary innovation. Although phenotypes resulting from loss of functions are well investigated (e.g., female sterility), little is known about the domains contributing to Hoxa‐11 protein function. We therefore mapped the domains mediating two essential transcription factor functions, nuclear localization and transcriptional activity in the mouse Hoxa‐11 protein. Our results show that the mammal‐specific alanine repeat does not contribute to repressor activity, as has been hypothesized based on amino acid composition and analogy with other repressor domains. Interestingly, both the repressor domain as well as the nuclear localization signal (NLS) are located within the homeodomain, adding to the growing evidence that the homeodomain is a multifunctional domain which fulfills essential transcription factor functions beyond DNA binding. It is proposed that the high degree of conservation of the homeodomain is due to the multiple functional constraints that result from the various conserved functions accommodated in the homeodomain. (Supplementary material for this article is available on the Journal of Experimental Zoology Website at http://www.interscience.wiley.com/jpages/1552‐5007/suppmat/index.html) J. Exp. Zool. (Mol. Dev. Evol.) 304B, 2005. © 2005 Wiley‐Liss, Inc. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Experimental Zoology Part A: Ecological and Integrative Physiology Wiley

Repressor domain and nuclear localization signal of the murine Hoxa‐11 protein are located in the homeodomain: no evidence for role of poly alanine stretches in transcriptional repression

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References (43)

Publisher
Wiley
Copyright
Copyright © 2005 Wiley Subscription Services, Inc., A Wiley Company
ISSN
2471-5638
eISSN
2471-5646
DOI
10.1002/jez.b.21061
pmid
16032701
Publisher site
See Article on Publisher Site

Abstract

Hoxa‐11 is a member of the homeodomain class of transcription factors, which play important roles in metazoan development. Hoxa‐11 is particularly interesting because it is involved in a major mammalian innovation, uterus development and gestation. We are interested in the molecular changes underlying this evolutionary innovation. Although phenotypes resulting from loss of functions are well investigated (e.g., female sterility), little is known about the domains contributing to Hoxa‐11 protein function. We therefore mapped the domains mediating two essential transcription factor functions, nuclear localization and transcriptional activity in the mouse Hoxa‐11 protein. Our results show that the mammal‐specific alanine repeat does not contribute to repressor activity, as has been hypothesized based on amino acid composition and analogy with other repressor domains. Interestingly, both the repressor domain as well as the nuclear localization signal (NLS) are located within the homeodomain, adding to the growing evidence that the homeodomain is a multifunctional domain which fulfills essential transcription factor functions beyond DNA binding. It is proposed that the high degree of conservation of the homeodomain is due to the multiple functional constraints that result from the various conserved functions accommodated in the homeodomain. (Supplementary material for this article is available on the Journal of Experimental Zoology Website at http://www.interscience.wiley.com/jpages/1552‐5007/suppmat/index.html) J. Exp. Zool. (Mol. Dev. Evol.) 304B, 2005. © 2005 Wiley‐Liss, Inc.

Journal

The Journal of Experimental Zoology Part A: Ecological and Integrative Physiology – Wiley

Published: Sep 15, 2005

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