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- Publisher
- Wiley
- Copyright
- © American Academy of Periodontology
- ISSN
- 0022-3492
- eISSN
- 1943-3670
- DOI
- 10.1902/jop.1987.58.8.540
- pmid
- 3305857
- Publisher site
- See Article on Publisher Site
Abstract
Recent immunohistological studies have suggested that Actinobacillus actinomycetemcomitans is present in the gingival tissues in juvenile periodontitis lesions. The present study examined tissue bound A. actinomycetemcomitans by bacterial culture and immunohistological demonstration of antigen in tissue. A total of 14 periodontitis lesions were examined. Eleven biopsies were obtained from gingiva adjacent to A. actinomycetemcomitans infected pockets, while the remaining three control biopsies were obtained from gingiva adjacent to pockets where subgingival A. actinomycetemcomitans infection could not be detected. Each biopsy was hemisected, one half was used for immunofluorescence microscopic examination while the other half was processed for culture of A. actinomycetemcomitans. The latter section was surface‐disinfected, repeatedly washed and then minced to release bacteria from within the tissues. Aliquots from the serial washings and the minced tissue suspension were cultured on medium selective for A. actinomycetemcomitans.
Journal
Journal of Periodontology – Wiley
Published: Aug 1, 1987