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Pathogenesis of non-alcoholic fatty liver disease mediated by YAP

Pathogenesis of non-alcoholic fatty liver disease mediated by YAP Objective This study aimed to investigate the mechanism of the interaction between Yes-associated protein (YAP) and transforming growth factor-b (TGF-b)/Smad signaling pathways in the development of non-alcoholic fatty liver disease (NAFLD). Methods Serum samples of monkeys with biopsy-proven NAFLD and healthy normal monkeys were used to measure fasting plasma glucose (FPG), low-density lipoprotein (LDL), high-density lipoprotein (HDL), triglyceride (TG) and albumin (ALB) with the BECKMAN CX5 PRO. Hematoxylin-eosin staining (H&E) was used for pathologic analysis, Masson trichrome staining was used to assess for fibrosis staging, and Oil Red O staining was used to detect lipid droplet deposition. According to an NAFLD activity score of\ 4 points and[ 4 points, the samples were divided into groups: the steatosis group and fibrosing NASH group. Furthermore, monkeys with a fibrosis stage \ 2 were assigned to the mild fibrosis group, while monkeys with fibrosis stage C 2 were assigned to the significant fibrosis group. Moreover, the fibrosis stage was subdivided as follows: stages 1a, 1c and 2–3. Immunohistochemistry and real-time quantitative PCR were used to quantify protein and gene expression, respectively. Results In the present study, 54 monkeys with NAFLD and 23 normal monkeys were recruited. Serum FPG and TG levels were http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Hepatology International Springer Journals

Pathogenesis of non-alcoholic fatty liver disease mediated by YAP

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References (25)

Publisher
Springer Journals
Copyright
Copyright © 2018 by Asian Pacific Association for the Study of the Liver
Subject
Medicine & Public Health; Hepatology; Colorectal Surgery; Surgery
ISSN
1936-0533
eISSN
1936-0541
DOI
10.1007/s12072-017-9841-y
pmid
29330836
Publisher site
See Article on Publisher Site

Abstract

Objective This study aimed to investigate the mechanism of the interaction between Yes-associated protein (YAP) and transforming growth factor-b (TGF-b)/Smad signaling pathways in the development of non-alcoholic fatty liver disease (NAFLD). Methods Serum samples of monkeys with biopsy-proven NAFLD and healthy normal monkeys were used to measure fasting plasma glucose (FPG), low-density lipoprotein (LDL), high-density lipoprotein (HDL), triglyceride (TG) and albumin (ALB) with the BECKMAN CX5 PRO. Hematoxylin-eosin staining (H&E) was used for pathologic analysis, Masson trichrome staining was used to assess for fibrosis staging, and Oil Red O staining was used to detect lipid droplet deposition. According to an NAFLD activity score of\ 4 points and[ 4 points, the samples were divided into groups: the steatosis group and fibrosing NASH group. Furthermore, monkeys with a fibrosis stage \ 2 were assigned to the mild fibrosis group, while monkeys with fibrosis stage C 2 were assigned to the significant fibrosis group. Moreover, the fibrosis stage was subdivided as follows: stages 1a, 1c and 2–3. Immunohistochemistry and real-time quantitative PCR were used to quantify protein and gene expression, respectively. Results In the present study, 54 monkeys with NAFLD and 23 normal monkeys were recruited. Serum FPG and TG levels were

Journal

Hepatology International – Springer Journals

Published: Jan 12, 2018

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