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Restriction and modification enzymes and their recognition sequences

Restriction and modification enzymes and their recognition sequences Downloaded from https://academic.oup.com/nar/article/9/1/213/1043263 by DeepDyve user on 31 July 2022 volume 9 Number i 1981 Nucleic Acids Research Richard J.Roberts Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA Introductio n Sinc e the last compilation of Type II restriction endonucleases (133), mor e than 45 new enzymes have been discovered. Among the valuable new specificitie s are Gdil and its isoschizomer StuI (AGGCCT), Gdill (PyGGCCG) , HgiEII (ACC[N] GGT), Rrul (AGTACT), Tthlll I and its isoschizomer s Ttrl and Ttel (GACNNNGTC), and Tthll l II (CAAPuCA). Th e new enzyme Neil (CC[G/C]GG) turns out to be an isoschizomer of CauI I whose recognition has recently been determined. The recognition sequence s of Snal (GTATAC) and Saul (CCTNAGG) have also been newly determined . Among the 258 enzymes listed, there are at least 69 different specificities . New entries , together with new information about recognition sequences , are indicated (§) . I n forming this list, all endonucleases cleaving DNA at a specific sequenc e have been considered to be restriction enzymes although, in most cases , there is no direct genetic evidence for the presenc e of a restriction modification system. These endonucleases are named in accordance with th e proposal of Smith and Nathans (161). Within the table, the source of eac h microorganism is given either as an individual or a National Culture Collection . If further information is required, it can be found either in th e first reference shown which, in each case, refers to the purification procedur e for the restriction enzyme, or from the individuals who have provide d their unpublished results. Where more than one reference appears , the second concerns the recognition sequence for the restriction enzyme , the third contains the purification procedure for the methylase, an d the fourth describes the recognition sequence for the methylase. In some cases, several references appear in one of these categories when independen t groups have reached similar conclusions. Reprints of this article are obtainable from the author at the above address. © IRL Press Limited, 1 Falconberg Court, London W1V 5FG. U.K. r75 Downloaded from https://academic.oup.com/nar/article/9/1/213/1043263 by DeepDyve user on 31 July 2022 a c licroorqanism Sequence sites References Source Enzyme Number of cleavaqe \ Ad2 SV40 0X174 § Acetobacter aceti sub. IFO 12388 GGATCC 5 3 1 0 150 AacI (BamHI) liquefaciens 1 150 § Acetobacter aceti sub. H. Van Montagu Aael (BamHI) GGATCC 5 3 0 liquefaciens 7 7 7 ATCC 15934 Aiml ? 40 Achromobacter immobilis 7 8 1 2 197 Acinetobacter calcoaceticus R.J. Roberts AccI GU(*)(^)AC >50 0 14 197 AceII (FnuDII) CGCG >50 AccIII >10 ? 124 § > 6 ? 149 Agrobacterium tumefaciens ATCC 15955 AtuAI >30 >30 16 2 140 Agrobacterium tumefaciens E. Nester AtuBI (EcoRII) CC(^)GG >35 >35 B6806 0 139 Agrobacterium tumefaciens G. Roizes AtuBVI ? >14 1 IIBV7 Atull (EcoRII) CC(*)GG >35 >35 16 2 Agrobacterium tumefaciens C. Kado ID 135 5 1 0 149 Agrobacterium tumefaciens E. Nester AtuCI (BcU) TGATCA 7 C58 7 7 7 ? 77 Anabaena catanula CCAP 1403/1 Acal 7 33 Anabaena cylindrica ATCC 27899 GPuiCGPyC >14 >14 0 Acyl >15 0 1 34 Anabaena oscillarioides CCAP 1403/11 AosI (MstI) TGCiGCA >10 >14 0 7 34 AosII (Acyl) GPuiCGPyC >14 >14 0 7 32 § Anabaena strain Waterbury ATCC 29208 AstWI (Acyl) GPuiCGPyC >14 >30 11 2 78 Anabaena subcylindrica CCAP 1043/4b Asul GiGNCC >30 0 124, 32; 32 TT+CGAA 7 1 0 AsuII 0 7 32 AsuIII (Acyl) GPuiCGPyC >14 >14 § Downloaded from https://academic.oup.com/nar/article/9/1/213/1043263 by DeepDyve user on 31 July 2022 Microorqamsm Source Enzyme Sequence Number of cleavage sites References \ Ad2 SV40 <t>X174 Anabaena variabilis ATCC 27892 Aval C+PyCGPuG 15 0 1 121; 79 Avail G*G(*)CC >17 >30 6 1 121; 173, 79, 45 •> 0 138; 138, 155 AvalII ATGCAT 3 Anabaena variabilis " E.C. Rosenvold Avrl (Aval) CPyCGPuG 8 15 0 1 141 Avrll 2 2 2 0 141 CCTAGG Arthrobacter luteus AG+CT >50 >50 35 24 135 ATCC 21606 Alul Arthrobacter pyridinolis R. DiLauro Apyl (EcoRII) CQ(*)GG >35 >35 16 2 35 1 112, 124 Bacillus acidocaldarius ATCC 27009 BacI (SacII) CCGCGG 4 >25 0 BamFl (BaitiHI) GGATCC 5 3 1 0 154 Bacillus amyloliquefaciens F ATCC 23350 BamHI G+GATCC 5 3 1 0 190; 137; 69; 69 Bacillus amyloliquefaciens H F.E. Young 5 3 1 0 154 Bacillus amyloliquefaciens K T. Kaneko BamKI (BamHI) GGATCC 3 1 0 153 Bacillus amyloliquefaciens N T. Ando BamNI (BamHI) GGATCC 5 1 152, 153; 82 BamN (Avail) G+G(*)CC >17 >30 6 methylase— 185 Bacillus brevis S A. P. Zarubina BbvSI GC(*)GC —specific 14 54; 53; 69; 69 Bacillus brevis ATCC 9999 Bbvl GC(^)GC >30 >30 23 A. Atkinson Bell T+GATCA 7 5 1 0 10 Bacillus caldolyticus ? ? >10 ? ? 154 Bacillus cereus ATCC 14579 Bcel4579 ? >10 ? ? 154 Bacillus cereus IAH 1229 Bcel229 Bcel70 (PstI) CTGCAG 18 25 2 1 Bacillus cereus T. Ando >50 0 14 154 BceR (FnuDII) CGCG >50 Bacillus cereus Rf sm st T. Ando Downloaded from https://academic.oup.com/nar/article/9/1/213/1043263 by DeepDyve user on 31 July 2022 Microorqanism Source Enzyme Sequence Number of cleavaqe sites References Ad2 SV40 i>X174 h. Bacillus globigii G.A. Wilson Bgll GCCNNNN+NGGC 22 12 1 0 37, 191; 6, 96, 181 Bq_UI AlGATCT 12 0 0 6 37, 191; 128 7 7 7 Bacillus megaterium 899 B899 Bme899 154 ? > 5 1 ? 7 Bacillus megaterium B205-3 T. Kaneko Broe205 >10 ? 154 •> ? Bacillus megaterium J. Upcroft Bmel >10 >20 4 50 Bacillus pumilus AHU1387A T. Ando Bpul ? 6 >30 2 ? 81 Bacillus sphaericus IAM 1286 Bspl286 154 ? ? ? ? ? Bacillus sphaericus R P. Venetianer BsgRI (Haelll) GGCC >50 >50 19 11 87 Bacillus stearothemophilus N. Welker Bstl (BamHI) G*GATCC 5 3 1 0 23; 26 1503-4R 7 7 Bacillus stearothennophilus BstAI A. Atkinson 12 ? ? 7 7 Bacillus stearothermophilus N. Welker BstEI ? ? ? 114 CT C 1 BstEII G^GTNACC 11 8 0 0 114 BstEIII (Mbol) GATC >50 >50 > 8 0 114; 58; 124 Bacillus stearothermophilus ATCC 12980 BstPI (BstEII) G+GTNACC 11 8 0 0 130 BstNI (EcoRII) >35 16 2 148 Bacillus stearothermophilus D. Comb >35 >50 19 11 157 § Bacillus stearothermophilus T. Oshima Bsel (Haelll) GGCC >50 ct rain A97 b trd 1 N Oc£. Bsell (Hpal) GTTAAC 13 6 4 3 Bacillus subtilis strain X5 T. Trautner BsuRI (Haelll) GGtCC >50 >50 19 16; 17; 62 7 7 Bacillus subtilis Marburg 168 T. Ando BsuH >10 7 7 Bacillus subtilis ATCC 6633 BSU6663 >20 ? 154 Downloaded from https://academic.oup.com/nar/article/9/1/213/1043263 by DeepDyve user on 31 July 2022 Microorganism Source Enzyme Sequence Number of cleavaqe sites" References A Ad2 SV40 ([1X174 Bacillus subtilis IAM 1076 BsulO76 (Haelll) GGCC >50 >50 19 11 154 Bacillus subtilis IAM 1111 Bsulll4 (Haelll) GGCC >50 >50 19 11 154 Bacillus subtilis IAM 1247 Bsul247 (PstI) CTGCAG 18 25 2 1 154; 75 7 7 7 Bacillus subtilis ATCC 14593 Bsull45 >20 154 •> 7 Bacillus subtilis IAM 1192 Bsull92 >10 ? ? 154 •> 7 ? 7 Bacillus subtilis IAM 1193 Bsull93 >30 154 •> ? Bacillus subtilis Bsul231 >20 ? ? 154 IAM 1231 ? 7 Bacillus subtilis ? ? 154 IAM 1259 Bsul2S9 > 8 11 0 124 Bordetella bronchiseptica ATCC 19395 BbrI (HindHI) AAGCTT 6 6 § Bordetella pertussis P. Novotny Bgel (HindHI) AAGCTT 6 11 6 0 59 Brevibacterium albidum ATCC 15831 Ball TGGiCCA 15 17 0 0 51 Brevibacterium luteum ATCC 15830 Blul (Xhol) C+TCGAG 1 6 0 55 11 182 BluII (Haelll) GGCC >5O >50 19 Caryophanon latum L Clal ATiCGAT 12 2 0 0 110 H. Mayer GGCC >50 >50 19 11 164 § Caryophanon latum ATCC 15219 Clml (Haelll) >30 6 1 164 Clmll (Avail) GG(^)CC >17 11 112 Caryophanon latum DSM 484 Cltl (Haelll) GG*CC >50 >50 19 Chloroflexus aurantiacus A. Bingham Caul (Avail) GG(*)CC >30 >30 6 1 11 >30 >30 0 1 11, 100 § Caul I CCK{j)GG 7 ? 7 7 Chromobacterium violaceum ATCC 12472 Cvil Clostridium formicoaceticum ATCC 23439 Cfol (Hhal) GCGC >50 >50 2 18 106 Downloaded from https://academic.oup.com/nar/article/9/1/213/1043263 by DeepDyve user on 31 July 2022 Microorganism Source Enzyme Number of cleavage sites References Corynebacterium humiferum ATCC 21108 Chul (HindlH) 40 ChuII (HindiI) 40 Corynebacterium petrophilum ATCC 19080 Cpel (Bell) 43 Desulfovibrio desulfuricans H. Peck Ddel 106; 52 Norway strain § Ddell (Xhol) 124 § Desulfovibrio desulfuricans ATCC 27774 Ddsl (BamHI) 105 Diplococcus pneumoniae S. Lacks Dpnl 91; 48, 92 —only cleaves methylated DNA-- Diplbcoccus pneumoniae S. Lacks DpnII (Mbol) 91; 92 Enterobacter cloacae H. Hartmann Ecu EcUI (EcoRII) Enterobacter cloacae DSH 30056 Ecal (BstEII) 74 CC(*)GG Ecall (EcoRII) 124 Enterobacter cloacae DSM 30060 EccI (SacII) CCGCGG 111; 124 61; 70; 61; 36 Escherichia coli RY13 R.N. Yoshimori EcoRI GiAATTC EcoRI' 120 196; 8, 13; 196; 13 +CC(*)GG Escherichia coli R245 R.N. Yoshimori EcoRII Escherichia coli B W. Arber TGA(N) TGCT 42; 94, 131; 95; 184 EcoB g Escherichia coli K H. Meselson AAC(N) GTGC 115; 7, 84; 64 EcoK 6 Escherichia coli (PI) K. Murray EcoPI AGACC 63; 4; 14, 15; 4, 68 132; 65 Escherichia coli P15 W. Arber EcoPI5 CAGCAG Flavobacterium species N. Brown FspAI (BstEII) GtGTNACC Downloaded from https://academic.oup.com/nar/article/9/1/213/1043263 by DeepDyve user on 31 July 2022 Microorganism Source Enzyme Number of cleavage sites References SV40 Fusobacterium nucleatum A M. Smith FnuAI (Hinfl) >55 102 TF FnuAII (Mbol) 0 102; 124 >50 >50 Fusobacterium nucleatum C M. Smith FnuCI (Mbol) o 102 >50 >50 Fusobacterium nucleatum D M. Smith FnuDI (Haelll) >50 n 102 >50 FnuDII >50 14 102 >50 FnuDI11 (Hhal) >50 >50 18 102 >50 Fusobacterium nucleatum E M. Smith FnuEI (Mbol) >50 0 102 •> Fusobacterium nucleatum 48 M. Smith Fnu48 I >50 >10 101 Fusobacterium nucleatum 4H M. Smith Fnu4H I >50 >50 25 31 99 § Gluconobacter dioxy- IAM 1814 Gdil (StuI) > 6 7 1 182 acetonicus 0 2 182 1 0 150 § Gluconobacter dioxy- IAM 1840 Gdol (BamHI) acetonicus Goxl (BamHI) 150 § Gluconobacter oxydans IAM 1836 GGATCC sub. melonogenes Haemophilus aegyptius ATCC 11116 Hael ()GG+CC() 6 123 PuGCGCiPy >30 >30 1 8 134; 180 Haell GG*CC >50 >50 19 11 116; 17; 108; 108 Haelll ? ? •> •> Haemophilus aphrophilus ATCC 19415 >30 124 Hap I CKGG >50 >50 5 176; 170 HapII (Hpall) GACGC >50 >50 0 14 176; 20, 169 Haemophilus gallinarum ATCC 14385 Hgal Downloaded from https://academic.oup.com/nar/article/9/1/213/1043263 by DeepDyve user on 31 July 2022 Microorganism Sequence Number of cleavaqe sites Reference•s Source Enzyme \ SV40 Ad2 JK1M Haemophilus haemo- ATCC 19416 Hhgl (HaelH) GGCC >50 TF 124 globinophilus 18 136; 136; 109 Haemophilus haemolyticus ATCC 10014 Hhal GCGiC >50 >50 2 HhalI (Hinfl) GANTC >50 >50 10 21 107 >50 18 165 and 25 § Haemophilus influenzae GU J. Chirikjian HinGU I (Hhal) GCGC >50 2 § HinGU II ? >50 >50 12 > 8 165 § Haemophilus influenzae 173 J. Chirikjian Hinl73 (Hindlll) AAGCTT 6 6 0 165 Haemc/philus influenzae 1056 J. Stuy HinlO56I (FnuDII) CGCG >50 >50 0 126 ? >30 >3O 0 5 126 HinlO56II Haemophilus influenzae J. Stuy Hinblll (Hindlll) AAGCTT 6 11 6 0 126 serotype b, 1076 AAGCTT 11 6 0 117, 124 Haemophilus influenzae R, C.A. Hutchison Hnibin (Hindlll) 6 GTPyPuAC 34 >20 7 13 126 Haemophilus influenzae J. Stuy Hindi (Hindll) serotype c, 1160 Haemophilus influenzae J. Stuy Hindi (Hindll) GTPyPuAC 34 >20 7 13 126 serotype c, 1161 >20 7 13 93 Haemophilus influenzae R A. Landy, Hindi (Hindll) GTPyPuAC 34 G. Leidy —specific 142; 143 Haemophilus influenzae R. S.H..Goodgal Hindi CAC methylase— (exo mutant) GTPyiPuAC 34 >20 7 13 162, 86, 142, 143 Hindll /UAGCTT 6 11 6 0 125; 125 ; 142; 143 Hindlll —specific methylase— 142; 143 HindIV GAC G+ANTC >50 >50 10 21 117; 80, 122 Haemophilus influenzae R C.A. Hutchison Hinfl 11 6 0 109 HinflI (Hindlll) AAGCTT 6 <— 0 5 85, 127 Hinflll CGAAT —Type III Downloaded from https://academic.oup.com/nar/article/9/1/213/1043263 by DeepDyve user on 31 July 2022 Microorganism Enzyme Number of cleavage sites'" References Source \ M2 SV40 <{>X174 Haemophilus influenzae H-l M. Takanami HinHI (Haell) ~>3O 176 >3l3 Haemophilus parahaemolyticus C.A. Hutchison HphI 9 117; 88 GGTGA >50 >50 Haemophilus parainfluenzae J. Setlow Hpal GTUAAC 13 6 3 151; 47, 2 Hpall UCGG >50 5 151; 47; 108; 108 >50 Haemophilus suis ATCC 19417 Hsul (Hindlll) A*AGCTT 11 0 124 Herpetosiphon giganteus J.H. Parish HgiAI G(*)GC(*)*C >20 3 22 HP1023 Herpetosiphon giganteus H. Reichenbach HgiBI (Avail) G+G(*)CC >30 1 73 Hpg 5 Herpetosiphon giganteus H. Reichenbach HqiCI GiGPyPuCC 73, 89 Hpg 9 Hgj.CH (Avail) GiG(*)CC >30 6 73 HgjCIII (Sail) GiTCGAC 3 0 73 >14 0 73, 89 Herpetosiphon giganteus H. Reichenbach HaiDI (Acy_I) GPuiCGPyC Hpa2 3 0 73 HgiDII (SaU) GiTCGAC HgiEI (Avail) 6 73 Herpetosiphon giganteus H. Reichenbach G*G( )CC >30 Hpg 24 HqiEII 1 ACC(N) GGT 73 HgiGI (Acyl) 0 7 § Herptosiphon giganteus H. Reichenbach GPu+CGPyC 89 Hpa 1 Klebsiella pneumoniae 0K8 J. Davies Kpnl GGTAUC 8 1 0 160; 178 Mlal (AsuII) TT+CGAA 0 0 38 § Hastigocladus laminosus CCAP 1447/1 1 TGCGCA 0 1 28; 53 Microcoleus species D. Comb HstI >15 0 0 147 Mstll > 6 8 0 49 Moraxel la bovis ATCC 10900 Hbol +GATC >50 >5O 16 11 MboII GAAGA >50 >5O 49; 19, 39 Downloaded from https://academic.oup.com/nar/article/9/1/213/1043263 by DeepDyve user on 31 July 2022 Microorganism Source Enzyme Sequence Number of cleavaqe sites References A Ad2 SV40 0X174 § Moraxeila bovis ATCC 17947 Mbvl ? ? 83 7 7 ? 7 Moraxeila glueidi LG1 J. Davies ? 160 Mali 7 7 ? Horaxella glueidi LG2 ? J. Davies Main ? 160 § Moraxeila kingae ATCC 23331 MkiI (Hindlll) AAGCTT 6 11 6 0 83 Moraxeila nonliquefaciens ATCC 19975 Mnol (Hpall) C+CGG >50 >50 1 5 124; 5 7 7 > 6 MnoII (Mnnlll) >10 3 124 >50 8 0 124 MnolII (Mbol) GATC >50 Moraxeila nonliquefaciens ATCC 17953 Mnll CCTC >50 >50 51 34 198 Moraxeila nonliquefaciens ATCC 17954 MnnI (Hindll) GTPyPuAC 34 >20 7 13 66 >50 Mrmll (Haelll) GGCC >5O 19 11 66 7 7 MnnI11 >10 > 6 3 66 >50 >50 2 18 66 MnnlV (Hhal) GCGC § Horaxella nonliquefaciens ATCC 19996 Mnil (Haelll) >50 19 11 83 GGCC >50 >50 1 5 83 Mrnll (Hp_aII) CCGG >50 >50 8 0 49 Moraxeila osloensis ATCC 19976 MosI (Mbol) GATC >50 >35 16 2 83 § Moraxeila phenylpyruvica ATCC 17955 MphI (EcoRII) CC(*)GG >35 >50 183, 147 Moraxeila species R.J. Roberts Ms£l (Hfiall) C*CGG >50 1 5 117, 124 Myxococcus stipitatus Mxs2H H. Reichenbach Hsil (Xhol) CTCGAG 1 & 0 1 7 7 7 Myxococcus virescens V-2 H. Reichenbach Mvil 1 119 7 7 ? ? 119 Mvill >15 1 § Neisseria cinerea NRCC 31006 Neil (CauII) CC(£)GG >15 0 189 Downloaded from https://academic.oup.com/nar/article/9/1/213/1043263 by DeepDyve user on 31 July 2022 a c Microorganism Source Enz.yne Sequence Number of cleavage sites References Ad2 SV40 iX174 Neisseria gonorrhoea G. Wilson Nqol (Haell) PuGCGCPy >30 192 ^3 0 8~ Neisseria gonorrhoeo CDC 66 NgoII (Haelll) GGCC >50 >50 19 11 29, 30 Oerskovia xanthineolytica R. Shekman >50 Oxal (Alul) AGCT >50 35 24 167 7 ? •> 7 Oxal I 167 Proteus vulgaris ATCC 13315 CGAUCG 7 0 Pvul 3 0 56 Pvul I CAG4-CTG 15 22 3 0 56 Providencia alcalifaciens ATCC 9886 Pall (Haelll) GGCC >50 >50 19 11 50 Providencia stuartii 164 J. Davies PstI CTGCAiG 18 25 2 1 160; 21 ? ? Pseudomonas aeruginosa G.A. Jacoby PaeR7 1 0 0 72 Pseudomonas facilis M. VanMontagu Pfal (Mbol) GATC >50 >50 8 0 183 Pseudomonas maltophila D. Comb Pmal CTGCAG 18 25 2 1 147 (PstO ? ? § Rhizobium leguminosarum 300 J. Beringer Rlel 6 >10 ? 194 •> 7 § Rhizobium lupini #1 W. Heumann Rlul 1 8 ? 193, 71 j 7 § Rhizobium meliloti J.L. Denarie Rmel ? 8 >10 71 § Rhodospirill urn rubrum A. deWaard AGT4-ACT ? 0 0 31 Rrul § RruII (EcoRII) CCi(|)GG >35 >35 16 2 31 § RhodospiriHum rubrum J. Chirikjian ? ? 4 5 1 98 Rrbl Rhodopseudomonas sphaeroides R. Lascelles Rspl (Pvul) CGATCG 3 7 0 9 Rhodopseudomonas sphaeroides S. Kaplan (Pvul) CGAT+CG 3 7 0 0 103 RshI Rhodopseudomonas sphaeroides S. Kaplan Rsal GT+AC >50 >50 11 11 104 5 1 0 46 Rhodopseudomonas sphaeroides S. Kaplan RsrI (EcoRI) GAATTC 5 Downloaded from https://academic.oup.com/nar/article/9/1/213/1043263 by DeepDyve user on 31 July 2022 Microorganism Source Enzyme Sequence Number of cleavaqe sites References A SV40 j Ad2 Serratia marcescens S. C. Mulder Smal CCCiGGG 3 hr~ 60; 41 •> •> Serratia species SAI B. Torheim SS£l ? ? ? 179 •> i Sphaerotilus natans C A. Pope Snal GTATAC 2 0 0 129 § Spiroplasma citri ASP2 M.A. Stephens SciNI (Hhal) G+CGC >50 >50 2 18 163 Staphylococcus aureus 3A E.E. Stobberingh >50 0 Sau3A (Mbol) +GATC >50 8 171 Staphylococcus aureus PS9G E.E. Stobberingh Sau9GI (Asul) GiGNCC >30 >30 11 2 172 Streptococcus faecal is >50 11 195 R. Wu Sfal (Haelll) GG4CC >50 19 var. zymogenes j Streptococcus faecal is GU J. Chirikjian SfaGU I (Hpall) CCGG >50 >50 1 5 27 Streptococcus faecal is ND547 GATGC >30 12 149 D. Clewell SfaNI >50 6 Streptomyces achromogenes ATCC 12767 Sac I GAGCT+C 2 0 0 1 SacII CCGUGG 4 >25 0 1 1 1 t SacIII ? >100 >100 1 25 2 1 24 Streptomyces albus CM1 527G6 SaJPI (PstI) CTGCAG 18 CTCGAG 1 G 0 1 175 Streptomyces albus KCC S0166 Sgal (Xhol) subspecies pathocidi'cus GiTCGAC 2 3 0 0 3 Streptomyces albus G J.M. Ghuysen SaJI •> Sal 11 ? >20 ? ? § Streptomyces aureofaciens J. Timko Saul CC4JNAGG 2 7 0 0 177 IKA 18/4 1 174 Streptomyces bobili ATCC 3310 Sbol (SacII) CCGCGG 4 >25 0 6 0 1 174 Streptomyces cupidosporus KCC S031G Scul (Xhol) CTCGAG 1 1 175 Streptomyces exfoliatus KCC S0030 SexI (Xhol) CTCGAG 1 6 0 Downloaded from https://academic.oup.com/nar/article/9/1/213/1043263 by DeepDyve user on 31 July 2022 Microorganism Source Enzyme Sequence Number of cleavaqe sites References X SV40 <t>X174 Ad2 Streptomyces fradiae ATCC 3355 Sfrl (SacII) CCGCGG ~0 ~ 174 4" Streptomyces goshikiensis KCC S0294 CTCGAG 1 6 0 1 175 Sgol (Xhol) •> ? 0 7 0 1 Streptomyces griseus ATCC 23345 SgrI Shyl (SacII) 4 0 1 187 Streptomyces hygroscopicus F. Walter CCGCGG >25 1 6 0 1 174 Streptomyces laveodulae ATCC 8664 Slal (Xhol) UTCGAG 1 6 0 1 175 Streptomyces luteoreticuli KCC S0788 Slul (Xhol) CTCGAG Streptomyces phaeochromogenes F. Bolivar SphI GCATG*C 4 9 2 0 44 Streptomyces Stanford S. Goff, SstI (SacI) GAGCTiC 2 7 0 0 57; 118 M. namuacn Sstll (SacII) CCGC+GG 4 >25 0 1 57 •> Sstlll (SacIII) >100 >100 1 76 SstIV (BcJI) TGATCA 7 5 0 § Streptomyces tubercidicus S. Takahashi StuI AGGiCCT >10 > 6 7 1 156 Thai (FnuDII) CGiCG >50 >50 0 14 113 Thermoplasma acidophilum D. Searcy CCGCGG 3 >25 0 1 54 Thermopolyspora glauca ATCC 15345 M I (SacII) TiCGA >50 >50 1 10 144; 144; -; 145 Thermus aquaticus YTI J.I. Harris TaqI •> >30 >30 4 6 124 Tagil TCGA >5O >50 1 10 145 § Thermus flavus AT62 T. Oshima Tfll (TagI) 146, 186; 146, 186; 145; 145 TCGA >50 >50 1 10 § Thermus thermophilus HB8 T. Oshima TthHB8 I (TaqI) •> 0 0 159 § Thermus thermophilus T. Oshima Ttrl (Tthlll I) GACNNNGTC 2 strain 23 0 159 Ttel (Tthlll I) GACNNNGTC 2 0 § Thermus thermophilus T. Oshima strain 110 Downloaded from https://academic.oup.com/nar/article/9/1/213/1043263 by DeepDyve user on 31 July 2022 Microorqanism Source Enzyme Sequence Number of cleavage sites References Ad2 SV40 < § Thermus thermophilus T. Oshima Tthlll I GACfUNNGTC I 5 159, 159 strain 111 Tthlll II CAAPuCA ' >30 >30 158, 158 •> •) § Tthlll III ? ? ? 157 § Tolypothrix tenuis W. Siegelman Ttnl (Haelll) GGCC >50 >50 11 19 168 Xanthomonas amaranthicola ATCC 11645 XamI (Sail) GTCGAC 3 0 2 0 3 Xanthomonas badrii ATCC 11672 Xbal T4CTAGA l 4 0 0 199 Xanthomonas holcicola ATCC 13461 Xhol CiTCGAG 1 6 0 1 55 Xhol I PuiGATCPy >20 >20 3 0 126; 54 Xanthomonas malvacearum ATCC 9924 Xmal (Smal) DCCGGG 3 12 0 0 41 Xmall (PstI) CTGCAG 18 25 2 1 41 XmalII CiGGCCG 2 10 0 0 Xanthomonas nigromaculans ATCC 23390 Xrnl (Pvul) CGATCG 3 7 0 0 66 Xanthomonas oryzae M. Ehrlich Xorl (PstI) CTGCAG 18 25 2 1 188 Xorll (Pvul) CGATCiG 3 7 0 0 188 Xanthomonas papavericola ATCC 14180 Xpal (Xhol) DTCGAG 1 6 0 1 55 Downloaded from https://academic.oup.com/nar/article/9/1/213/1043263 by DeepDyve user on 31 July 2022 Footnotes to Table a. When two enzymes recognize the same sequence, i.e., are isoschizomers, the prototype (i.e., the first example isolated) is indicated in parentheses. 1 1 b . Recognition sequences are written from 5 •* 3 , only one strand being given, and the point of cleavage is indicated by an arrow (+). When no arrow appears, the precise cleavage site has not been determined. For example, GiGATCC is an abbreviation for 5 C'C A T C C 3' 3 C C T A G G 5' Bases appearing in parentheses signify that either base may occupy that position in the recognition sequence Thus , Accl cleaves the sequence GTAGAC, GTATAC, GTCGAC, and GTCTAC. Where known, the base modified by the corresponding specific methylase is indicated by an asterisk. A is N -methyladenosine. C is 5-methyl- cytosine. c. These columns indicate the frequency of cleavage by the various specific endonucleases on bacteriophage lambda DNA (K), adenovirus-2 DNA (Ad2), simian virus 40 DNA (SV40), and 0X174 Rf DNA «iX174). In the latter two cases, the sites were checked by computer search of the published sequences d. In most E. coli strains, bacteriophage lambda DNA is partially modified against the action of AtuBI, AtuII, AtuCI, Bell, BstEIII, Cgel, DpjiII, Ecall, EclII, EcoRII, FnuAII, FnuCI, Mbol, MnoIII, MosI, MphI, and Xbal. It should be noted" that FnuE I, Pfal and Sau3A are not inhibited by dam methylation; BstNI and Apyl are not inhibited by mec methylation. e. Hgal cleaves as indicated: 5 GACGCNNNNN* 3' 3' CTGCGNNNNNNNNNN 5' f. Hghl cleaves as indicated: 5' GGTGANNNNNNNN* 3 3 CCACTNNNNNNN 5' g. MboII cleaves as indicated: 5' GAAGANNNNNNNN* 3' , cTTCTNNNNNNN 5' 3 f h. Mnll cleaves 5 to 10 bases in the 3 direction from the recognition sequence. SfaNI cleaves an unknown number of nucleotides away from the recognition sequence. i. EcoPI, EcoP15, and Hinflll have characteristics intermediate between those of the Type I and Type II restriction endonucleases. 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Restriction and modification enzymes and their recognition sequences

Roberts, J. Richard
Nucleic Acids Research , Volume 9 (1) – Jan 10, 1981
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Downloaded from https://academic.oup.com/nar/article/9/1/213/1043263 by DeepDyve user on 31 July 2022 volume 9 Number i 1981 Nucleic Acids Research Richard J.Roberts Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA Introductio n Sinc e the last compilation of Type II restriction endonucleases (133), mor e than 45 new enzymes have been discovered. Among the valuable new specificitie s are Gdil and its isoschizomer StuI (AGGCCT), Gdill (PyGGCCG) , HgiEII (ACC[N] GGT), Rrul (AGTACT), Tthlll I and its isoschizomer s Ttrl and Ttel (GACNNNGTC), and Tthll l II (CAAPuCA). Th e new enzyme Neil (CC[G/C]GG) turns out to be an isoschizomer of CauI I whose recognition has recently been determined. The recognition sequence s of Snal (GTATAC) and Saul (CCTNAGG) have also been newly determined . Among the 258 enzymes listed, there are at least 69 different specificities . New entries , together with new information about recognition sequences , are indicated (§) . I n forming this list, all endonucleases cleaving DNA at a specific sequenc e have been considered to be restriction enzymes although, in most cases , there is no direct genetic evidence for the presenc e of a restriction modification system. These endonucleases are named in accordance with th e proposal of Smith and Nathans (161). Within the table, the source of eac h microorganism is given either as an individual or a National Culture Collection . If further information is required, it can be found either in th e first reference shown which, in each case, refers to the purification procedur e for the restriction enzyme, or from the individuals who have provide d their unpublished results. Where more than one reference appears , the second concerns the recognition sequence for the restriction enzyme , the third contains the purification procedure for the methylase, an d the fourth describes the recognition sequence for the methylase. In some cases, several references appear in one of these categories when independen t groups have reached similar conclusions. Reprints of this article are obtainable from the author at the above address. © IRL Press Limited, 1 Falconberg Court, London W1V 5FG. U.K. r75 Downloaded from https://academic.oup.com/nar/article/9/1/213/1043263 by DeepDyve user on 31 July 2022 a c licroorqanism Sequence sites References Source Enzyme Number of cleavaqe \ Ad2 SV40 0X174 § Acetobacter aceti sub. IFO 12388 GGATCC 5 3 1 0 150 AacI (BamHI) liquefaciens 1 150 § Acetobacter aceti sub. H. Van Montagu Aael (BamHI) GGATCC 5 3 0 liquefaciens 7 7 7 ATCC 15934 Aiml ? 40 Achromobacter immobilis 7 8 1 2 197 Acinetobacter calcoaceticus R.J. Roberts AccI GU(*)(^)AC >50 0 14 197 AceII (FnuDII) CGCG >50 AccIII >10 ? 124 § > 6 ? 149 Agrobacterium tumefaciens ATCC 15955 AtuAI >30 >30 16 2 140 Agrobacterium tumefaciens E. Nester AtuBI (EcoRII) CC(^)GG >35 >35 B6806 0 139 Agrobacterium tumefaciens G. Roizes AtuBVI ? >14 1 IIBV7 Atull (EcoRII) CC(*)GG >35 >35 16 2 Agrobacterium tumefaciens C. Kado ID 135 5 1 0 149 Agrobacterium tumefaciens E. Nester AtuCI (BcU) TGATCA 7 C58 7 7 7 ? 77 Anabaena catanula CCAP 1403/1 Acal 7 33 Anabaena cylindrica ATCC 27899 GPuiCGPyC >14 >14 0 Acyl >15 0 1 34 Anabaena oscillarioides CCAP 1403/11 AosI (MstI) TGCiGCA >10 >14 0 7 34 AosII (Acyl) GPuiCGPyC >14 >14 0 7 32 § Anabaena strain Waterbury ATCC 29208 AstWI (Acyl) GPuiCGPyC >14 >30 11 2 78 Anabaena subcylindrica CCAP 1043/4b Asul GiGNCC >30 0 124, 32; 32 TT+CGAA 7 1 0 AsuII 0 7 32 AsuIII (Acyl) GPuiCGPyC >14 >14 § Downloaded from https://academic.oup.com/nar/article/9/1/213/1043263 by DeepDyve user on 31 July 2022 Microorqamsm Source Enzyme Sequence Number of cleavage sites References \ Ad2 SV40 <t>X174 Anabaena variabilis ATCC 27892 Aval C+PyCGPuG 15 0 1 121; 79 Avail G*G(*)CC >17 >30 6 1 121; 173, 79, 45 •> 0 138; 138, 155 AvalII ATGCAT 3 Anabaena variabilis " E.C. Rosenvold Avrl (Aval) CPyCGPuG 8 15 0 1 141 Avrll 2 2 2 0 141 CCTAGG Arthrobacter luteus AG+CT >50 >50 35 24 135 ATCC 21606 Alul Arthrobacter pyridinolis R. DiLauro Apyl (EcoRII) CQ(*)GG >35 >35 16 2 35 1 112, 124 Bacillus acidocaldarius ATCC 27009 BacI (SacII) CCGCGG 4 >25 0 BamFl (BaitiHI) GGATCC 5 3 1 0 154 Bacillus amyloliquefaciens F ATCC 23350 BamHI G+GATCC 5 3 1 0 190; 137; 69; 69 Bacillus amyloliquefaciens H F.E. Young 5 3 1 0 154 Bacillus amyloliquefaciens K T. Kaneko BamKI (BamHI) GGATCC 3 1 0 153 Bacillus amyloliquefaciens N T. Ando BamNI (BamHI) GGATCC 5 1 152, 153; 82 BamN (Avail) G+G(*)CC >17 >30 6 methylase— 185 Bacillus brevis S A. P. Zarubina BbvSI GC(*)GC —specific 14 54; 53; 69; 69 Bacillus brevis ATCC 9999 Bbvl GC(^)GC >30 >30 23 A. Atkinson Bell T+GATCA 7 5 1 0 10 Bacillus caldolyticus ? ? >10 ? ? 154 Bacillus cereus ATCC 14579 Bcel4579 ? >10 ? ? 154 Bacillus cereus IAH 1229 Bcel229 Bcel70 (PstI) CTGCAG 18 25 2 1 Bacillus cereus T. Ando >50 0 14 154 BceR (FnuDII) CGCG >50 Bacillus cereus Rf sm st T. Ando Downloaded from https://academic.oup.com/nar/article/9/1/213/1043263 by DeepDyve user on 31 July 2022 Microorqanism Source Enzyme Sequence Number of cleavaqe sites References Ad2 SV40 i>X174 h. Bacillus globigii G.A. Wilson Bgll GCCNNNN+NGGC 22 12 1 0 37, 191; 6, 96, 181 Bq_UI AlGATCT 12 0 0 6 37, 191; 128 7 7 7 Bacillus megaterium 899 B899 Bme899 154 ? > 5 1 ? 7 Bacillus megaterium B205-3 T. Kaneko Broe205 >10 ? 154 •> ? Bacillus megaterium J. Upcroft Bmel >10 >20 4 50 Bacillus pumilus AHU1387A T. Ando Bpul ? 6 >30 2 ? 81 Bacillus sphaericus IAM 1286 Bspl286 154 ? ? ? ? ? Bacillus sphaericus R P. Venetianer BsgRI (Haelll) GGCC >50 >50 19 11 87 Bacillus stearothemophilus N. Welker Bstl (BamHI) G*GATCC 5 3 1 0 23; 26 1503-4R 7 7 Bacillus stearothennophilus BstAI A. Atkinson 12 ? ? 7 7 Bacillus stearothermophilus N. Welker BstEI ? ? ? 114 CT C 1 BstEII G^GTNACC 11 8 0 0 114 BstEIII (Mbol) GATC >50 >50 > 8 0 114; 58; 124 Bacillus stearothermophilus ATCC 12980 BstPI (BstEII) G+GTNACC 11 8 0 0 130 BstNI (EcoRII) >35 16 2 148 Bacillus stearothermophilus D. Comb >35 >50 19 11 157 § Bacillus stearothermophilus T. Oshima Bsel (Haelll) GGCC >50 ct rain A97 b trd 1 N Oc£. Bsell (Hpal) GTTAAC 13 6 4 3 Bacillus subtilis strain X5 T. Trautner BsuRI (Haelll) GGtCC >50 >50 19 16; 17; 62 7 7 Bacillus subtilis Marburg 168 T. Ando BsuH >10 7 7 Bacillus subtilis ATCC 6633 BSU6663 >20 ? 154 Downloaded from https://academic.oup.com/nar/article/9/1/213/1043263 by DeepDyve user on 31 July 2022 Microorganism Source Enzyme Sequence Number of cleavaqe sites" References A Ad2 SV40 ([1X174 Bacillus subtilis IAM 1076 BsulO76 (Haelll) GGCC >50 >50 19 11 154 Bacillus subtilis IAM 1111 Bsulll4 (Haelll) GGCC >50 >50 19 11 154 Bacillus subtilis IAM 1247 Bsul247 (PstI) CTGCAG 18 25 2 1 154; 75 7 7 7 Bacillus subtilis ATCC 14593 Bsull45 >20 154 •> 7 Bacillus subtilis IAM 1192 Bsull92 >10 ? ? 154 •> 7 ? 7 Bacillus subtilis IAM 1193 Bsull93 >30 154 •> ? Bacillus subtilis Bsul231 >20 ? ? 154 IAM 1231 ? 7 Bacillus subtilis ? ? 154 IAM 1259 Bsul2S9 > 8 11 0 124 Bordetella bronchiseptica ATCC 19395 BbrI (HindHI) AAGCTT 6 6 § Bordetella pertussis P. Novotny Bgel (HindHI) AAGCTT 6 11 6 0 59 Brevibacterium albidum ATCC 15831 Ball TGGiCCA 15 17 0 0 51 Brevibacterium luteum ATCC 15830 Blul (Xhol) C+TCGAG 1 6 0 55 11 182 BluII (Haelll) GGCC >5O >50 19 Caryophanon latum L Clal ATiCGAT 12 2 0 0 110 H. Mayer GGCC >50 >50 19 11 164 § Caryophanon latum ATCC 15219 Clml (Haelll) >30 6 1 164 Clmll (Avail) GG(^)CC >17 11 112 Caryophanon latum DSM 484 Cltl (Haelll) GG*CC >50 >50 19 Chloroflexus aurantiacus A. Bingham Caul (Avail) GG(*)CC >30 >30 6 1 11 >30 >30 0 1 11, 100 § Caul I CCK{j)GG 7 ? 7 7 Chromobacterium violaceum ATCC 12472 Cvil Clostridium formicoaceticum ATCC 23439 Cfol (Hhal) GCGC >50 >50 2 18 106 Downloaded from https://academic.oup.com/nar/article/9/1/213/1043263 by DeepDyve user on 31 July 2022 Microorganism Source Enzyme Number of cleavage sites References Corynebacterium humiferum ATCC 21108 Chul (HindlH) 40 ChuII (HindiI) 40 Corynebacterium petrophilum ATCC 19080 Cpel (Bell) 43 Desulfovibrio desulfuricans H. Peck Ddel 106; 52 Norway strain § Ddell (Xhol) 124 § Desulfovibrio desulfuricans ATCC 27774 Ddsl (BamHI) 105 Diplococcus pneumoniae S. Lacks Dpnl 91; 48, 92 —only cleaves methylated DNA-- Diplbcoccus pneumoniae S. Lacks DpnII (Mbol) 91; 92 Enterobacter cloacae H. Hartmann Ecu EcUI (EcoRII) Enterobacter cloacae DSH 30056 Ecal (BstEII) 74 CC(*)GG Ecall (EcoRII) 124 Enterobacter cloacae DSM 30060 EccI (SacII) CCGCGG 111; 124 61; 70; 61; 36 Escherichia coli RY13 R.N. Yoshimori EcoRI GiAATTC EcoRI' 120 196; 8, 13; 196; 13 +CC(*)GG Escherichia coli R245 R.N. Yoshimori EcoRII Escherichia coli B W. Arber TGA(N) TGCT 42; 94, 131; 95; 184 EcoB g Escherichia coli K H. Meselson AAC(N) GTGC 115; 7, 84; 64 EcoK 6 Escherichia coli (PI) K. Murray EcoPI AGACC 63; 4; 14, 15; 4, 68 132; 65 Escherichia coli P15 W. Arber EcoPI5 CAGCAG Flavobacterium species N. Brown FspAI (BstEII) GtGTNACC Downloaded from https://academic.oup.com/nar/article/9/1/213/1043263 by DeepDyve user on 31 July 2022 Microorganism Source Enzyme Number of cleavage sites References SV40 Fusobacterium nucleatum A M. Smith FnuAI (Hinfl) >55 102 TF FnuAII (Mbol) 0 102; 124 >50 >50 Fusobacterium nucleatum C M. Smith FnuCI (Mbol) o 102 >50 >50 Fusobacterium nucleatum D M. Smith FnuDI (Haelll) >50 n 102 >50 FnuDII >50 14 102 >50 FnuDI11 (Hhal) >50 >50 18 102 >50 Fusobacterium nucleatum E M. Smith FnuEI (Mbol) >50 0 102 •> Fusobacterium nucleatum 48 M. Smith Fnu48 I >50 >10 101 Fusobacterium nucleatum 4H M. Smith Fnu4H I >50 >50 25 31 99 § Gluconobacter dioxy- IAM 1814 Gdil (StuI) > 6 7 1 182 acetonicus 0 2 182 1 0 150 § Gluconobacter dioxy- IAM 1840 Gdol (BamHI) acetonicus Goxl (BamHI) 150 § Gluconobacter oxydans IAM 1836 GGATCC sub. melonogenes Haemophilus aegyptius ATCC 11116 Hael ()GG+CC() 6 123 PuGCGCiPy >30 >30 1 8 134; 180 Haell GG*CC >50 >50 19 11 116; 17; 108; 108 Haelll ? ? •> •> Haemophilus aphrophilus ATCC 19415 >30 124 Hap I CKGG >50 >50 5 176; 170 HapII (Hpall) GACGC >50 >50 0 14 176; 20, 169 Haemophilus gallinarum ATCC 14385 Hgal Downloaded from https://academic.oup.com/nar/article/9/1/213/1043263 by DeepDyve user on 31 July 2022 Microorganism Sequence Number of cleavaqe sites Reference•s Source Enzyme \ SV40 Ad2 JK1M Haemophilus haemo- ATCC 19416 Hhgl (HaelH) GGCC >50 TF 124 globinophilus 18 136; 136; 109 Haemophilus haemolyticus ATCC 10014 Hhal GCGiC >50 >50 2 HhalI (Hinfl) GANTC >50 >50 10 21 107 >50 18 165 and 25 § Haemophilus influenzae GU J. Chirikjian HinGU I (Hhal) GCGC >50 2 § HinGU II ? >50 >50 12 > 8 165 § Haemophilus influenzae 173 J. Chirikjian Hinl73 (Hindlll) AAGCTT 6 6 0 165 Haemc/philus influenzae 1056 J. Stuy HinlO56I (FnuDII) CGCG >50 >50 0 126 ? >30 >3O 0 5 126 HinlO56II Haemophilus influenzae J. Stuy Hinblll (Hindlll) AAGCTT 6 11 6 0 126 serotype b, 1076 AAGCTT 11 6 0 117, 124 Haemophilus influenzae R, C.A. Hutchison Hnibin (Hindlll) 6 GTPyPuAC 34 >20 7 13 126 Haemophilus influenzae J. Stuy Hindi (Hindll) serotype c, 1160 Haemophilus influenzae J. Stuy Hindi (Hindll) GTPyPuAC 34 >20 7 13 126 serotype c, 1161 >20 7 13 93 Haemophilus influenzae R A. Landy, Hindi (Hindll) GTPyPuAC 34 G. Leidy —specific 142; 143 Haemophilus influenzae R. S.H..Goodgal Hindi CAC methylase— (exo mutant) GTPyiPuAC 34 >20 7 13 162, 86, 142, 143 Hindll /UAGCTT 6 11 6 0 125; 125 ; 142; 143 Hindlll —specific methylase— 142; 143 HindIV GAC G+ANTC >50 >50 10 21 117; 80, 122 Haemophilus influenzae R C.A. Hutchison Hinfl 11 6 0 109 HinflI (Hindlll) AAGCTT 6 <— 0 5 85, 127 Hinflll CGAAT —Type III Downloaded from https://academic.oup.com/nar/article/9/1/213/1043263 by DeepDyve user on 31 July 2022 Microorganism Enzyme Number of cleavage sites'" References Source \ M2 SV40 <{>X174 Haemophilus influenzae H-l M. Takanami HinHI (Haell) ~>3O 176 >3l3 Haemophilus parahaemolyticus C.A. Hutchison HphI 9 117; 88 GGTGA >50 >50 Haemophilus parainfluenzae J. Setlow Hpal GTUAAC 13 6 3 151; 47, 2 Hpall UCGG >50 5 151; 47; 108; 108 >50 Haemophilus suis ATCC 19417 Hsul (Hindlll) A*AGCTT 11 0 124 Herpetosiphon giganteus J.H. Parish HgiAI G(*)GC(*)*C >20 3 22 HP1023 Herpetosiphon giganteus H. Reichenbach HgiBI (Avail) G+G(*)CC >30 1 73 Hpg 5 Herpetosiphon giganteus H. Reichenbach HqiCI GiGPyPuCC 73, 89 Hpg 9 Hgj.CH (Avail) GiG(*)CC >30 6 73 HgjCIII (Sail) GiTCGAC 3 0 73 >14 0 73, 89 Herpetosiphon giganteus H. Reichenbach HaiDI (Acy_I) GPuiCGPyC Hpa2 3 0 73 HgiDII (SaU) GiTCGAC HgiEI (Avail) 6 73 Herpetosiphon giganteus H. Reichenbach G*G( )CC >30 Hpg 24 HqiEII 1 ACC(N) GGT 73 HgiGI (Acyl) 0 7 § Herptosiphon giganteus H. Reichenbach GPu+CGPyC 89 Hpa 1 Klebsiella pneumoniae 0K8 J. Davies Kpnl GGTAUC 8 1 0 160; 178 Mlal (AsuII) TT+CGAA 0 0 38 § Hastigocladus laminosus CCAP 1447/1 1 TGCGCA 0 1 28; 53 Microcoleus species D. Comb HstI >15 0 0 147 Mstll > 6 8 0 49 Moraxel la bovis ATCC 10900 Hbol +GATC >50 >5O 16 11 MboII GAAGA >50 >5O 49; 19, 39 Downloaded from https://academic.oup.com/nar/article/9/1/213/1043263 by DeepDyve user on 31 July 2022 Microorganism Source Enzyme Sequence Number of cleavaqe sites References A Ad2 SV40 0X174 § Moraxeila bovis ATCC 17947 Mbvl ? ? 83 7 7 ? 7 Moraxeila glueidi LG1 J. Davies ? 160 Mali 7 7 ? Horaxella glueidi LG2 ? J. Davies Main ? 160 § Moraxeila kingae ATCC 23331 MkiI (Hindlll) AAGCTT 6 11 6 0 83 Moraxeila nonliquefaciens ATCC 19975 Mnol (Hpall) C+CGG >50 >50 1 5 124; 5 7 7 > 6 MnoII (Mnnlll) >10 3 124 >50 8 0 124 MnolII (Mbol) GATC >50 Moraxeila nonliquefaciens ATCC 17953 Mnll CCTC >50 >50 51 34 198 Moraxeila nonliquefaciens ATCC 17954 MnnI (Hindll) GTPyPuAC 34 >20 7 13 66 >50 Mrmll (Haelll) GGCC >5O 19 11 66 7 7 MnnI11 >10 > 6 3 66 >50 >50 2 18 66 MnnlV (Hhal) GCGC § Horaxella nonliquefaciens ATCC 19996 Mnil (Haelll) >50 19 11 83 GGCC >50 >50 1 5 83 Mrnll (Hp_aII) CCGG >50 >50 8 0 49 Moraxeila osloensis ATCC 19976 MosI (Mbol) GATC >50 >35 16 2 83 § Moraxeila phenylpyruvica ATCC 17955 MphI (EcoRII) CC(*)GG >35 >50 183, 147 Moraxeila species R.J. Roberts Ms£l (Hfiall) C*CGG >50 1 5 117, 124 Myxococcus stipitatus Mxs2H H. Reichenbach Hsil (Xhol) CTCGAG 1 & 0 1 7 7 7 Myxococcus virescens V-2 H. Reichenbach Mvil 1 119 7 7 ? ? 119 Mvill >15 1 § Neisseria cinerea NRCC 31006 Neil (CauII) CC(£)GG >15 0 189 Downloaded from https://academic.oup.com/nar/article/9/1/213/1043263 by DeepDyve user on 31 July 2022 a c Microorganism Source Enz.yne Sequence Number of cleavage sites References Ad2 SV40 iX174 Neisseria gonorrhoea G. Wilson Nqol (Haell) PuGCGCPy >30 192 ^3 0 8~ Neisseria gonorrhoeo CDC 66 NgoII (Haelll) GGCC >50 >50 19 11 29, 30 Oerskovia xanthineolytica R. Shekman >50 Oxal (Alul) AGCT >50 35 24 167 7 ? •> 7 Oxal I 167 Proteus vulgaris ATCC 13315 CGAUCG 7 0 Pvul 3 0 56 Pvul I CAG4-CTG 15 22 3 0 56 Providencia alcalifaciens ATCC 9886 Pall (Haelll) GGCC >50 >50 19 11 50 Providencia stuartii 164 J. Davies PstI CTGCAiG 18 25 2 1 160; 21 ? ? Pseudomonas aeruginosa G.A. Jacoby PaeR7 1 0 0 72 Pseudomonas facilis M. VanMontagu Pfal (Mbol) GATC >50 >50 8 0 183 Pseudomonas maltophila D. Comb Pmal CTGCAG 18 25 2 1 147 (PstO ? ? § Rhizobium leguminosarum 300 J. Beringer Rlel 6 >10 ? 194 •> 7 § Rhizobium lupini #1 W. Heumann Rlul 1 8 ? 193, 71 j 7 § Rhizobium meliloti J.L. Denarie Rmel ? 8 >10 71 § Rhodospirill urn rubrum A. deWaard AGT4-ACT ? 0 0 31 Rrul § RruII (EcoRII) CCi(|)GG >35 >35 16 2 31 § RhodospiriHum rubrum J. Chirikjian ? ? 4 5 1 98 Rrbl Rhodopseudomonas sphaeroides R. Lascelles Rspl (Pvul) CGATCG 3 7 0 9 Rhodopseudomonas sphaeroides S. Kaplan (Pvul) CGAT+CG 3 7 0 0 103 RshI Rhodopseudomonas sphaeroides S. Kaplan Rsal GT+AC >50 >50 11 11 104 5 1 0 46 Rhodopseudomonas sphaeroides S. Kaplan RsrI (EcoRI) GAATTC 5 Downloaded from https://academic.oup.com/nar/article/9/1/213/1043263 by DeepDyve user on 31 July 2022 Microorganism Source Enzyme Sequence Number of cleavaqe sites References A SV40 j Ad2 Serratia marcescens S. C. Mulder Smal CCCiGGG 3 hr~ 60; 41 •> •> Serratia species SAI B. Torheim SS£l ? ? ? 179 •> i Sphaerotilus natans C A. Pope Snal GTATAC 2 0 0 129 § Spiroplasma citri ASP2 M.A. Stephens SciNI (Hhal) G+CGC >50 >50 2 18 163 Staphylococcus aureus 3A E.E. Stobberingh >50 0 Sau3A (Mbol) +GATC >50 8 171 Staphylococcus aureus PS9G E.E. Stobberingh Sau9GI (Asul) GiGNCC >30 >30 11 2 172 Streptococcus faecal is >50 11 195 R. Wu Sfal (Haelll) GG4CC >50 19 var. zymogenes j Streptococcus faecal is GU J. Chirikjian SfaGU I (Hpall) CCGG >50 >50 1 5 27 Streptococcus faecal is ND547 GATGC >30 12 149 D. Clewell SfaNI >50 6 Streptomyces achromogenes ATCC 12767 Sac I GAGCT+C 2 0 0 1 SacII CCGUGG 4 >25 0 1 1 1 t SacIII ? >100 >100 1 25 2 1 24 Streptomyces albus CM1 527G6 SaJPI (PstI) CTGCAG 18 CTCGAG 1 G 0 1 175 Streptomyces albus KCC S0166 Sgal (Xhol) subspecies pathocidi'cus GiTCGAC 2 3 0 0 3 Streptomyces albus G J.M. Ghuysen SaJI •> Sal 11 ? >20 ? ? § Streptomyces aureofaciens J. Timko Saul CC4JNAGG 2 7 0 0 177 IKA 18/4 1 174 Streptomyces bobili ATCC 3310 Sbol (SacII) CCGCGG 4 >25 0 6 0 1 174 Streptomyces cupidosporus KCC S031G Scul (Xhol) CTCGAG 1 1 175 Streptomyces exfoliatus KCC S0030 SexI (Xhol) CTCGAG 1 6 0 Downloaded from https://academic.oup.com/nar/article/9/1/213/1043263 by DeepDyve user on 31 July 2022 Microorganism Source Enzyme Sequence Number of cleavaqe sites References X SV40 <t>X174 Ad2 Streptomyces fradiae ATCC 3355 Sfrl (SacII) CCGCGG ~0 ~ 174 4" Streptomyces goshikiensis KCC S0294 CTCGAG 1 6 0 1 175 Sgol (Xhol) •> ? 0 7 0 1 Streptomyces griseus ATCC 23345 SgrI Shyl (SacII) 4 0 1 187 Streptomyces hygroscopicus F. Walter CCGCGG >25 1 6 0 1 174 Streptomyces laveodulae ATCC 8664 Slal (Xhol) UTCGAG 1 6 0 1 175 Streptomyces luteoreticuli KCC S0788 Slul (Xhol) CTCGAG Streptomyces phaeochromogenes F. Bolivar SphI GCATG*C 4 9 2 0 44 Streptomyces Stanford S. Goff, SstI (SacI) GAGCTiC 2 7 0 0 57; 118 M. namuacn Sstll (SacII) CCGC+GG 4 >25 0 1 57 •> Sstlll (SacIII) >100 >100 1 76 SstIV (BcJI) TGATCA 7 5 0 § Streptomyces tubercidicus S. Takahashi StuI AGGiCCT >10 > 6 7 1 156 Thai (FnuDII) CGiCG >50 >50 0 14 113 Thermoplasma acidophilum D. Searcy CCGCGG 3 >25 0 1 54 Thermopolyspora glauca ATCC 15345 M I (SacII) TiCGA >50 >50 1 10 144; 144; -; 145 Thermus aquaticus YTI J.I. Harris TaqI •> >30 >30 4 6 124 Tagil TCGA >5O >50 1 10 145 § Thermus flavus AT62 T. Oshima Tfll (TagI) 146, 186; 146, 186; 145; 145 TCGA >50 >50 1 10 § Thermus thermophilus HB8 T. Oshima TthHB8 I (TaqI) •> 0 0 159 § Thermus thermophilus T. Oshima Ttrl (Tthlll I) GACNNNGTC 2 strain 23 0 159 Ttel (Tthlll I) GACNNNGTC 2 0 § Thermus thermophilus T. Oshima strain 110 Downloaded from https://academic.oup.com/nar/article/9/1/213/1043263 by DeepDyve user on 31 July 2022 Microorqanism Source Enzyme Sequence Number of cleavage sites References Ad2 SV40 < § Thermus thermophilus T. Oshima Tthlll I GACfUNNGTC I 5 159, 159 strain 111 Tthlll II CAAPuCA ' >30 >30 158, 158 •> •) § Tthlll III ? ? ? 157 § Tolypothrix tenuis W. Siegelman Ttnl (Haelll) GGCC >50 >50 11 19 168 Xanthomonas amaranthicola ATCC 11645 XamI (Sail) GTCGAC 3 0 2 0 3 Xanthomonas badrii ATCC 11672 Xbal T4CTAGA l 4 0 0 199 Xanthomonas holcicola ATCC 13461 Xhol CiTCGAG 1 6 0 1 55 Xhol I PuiGATCPy >20 >20 3 0 126; 54 Xanthomonas malvacearum ATCC 9924 Xmal (Smal) DCCGGG 3 12 0 0 41 Xmall (PstI) CTGCAG 18 25 2 1 41 XmalII CiGGCCG 2 10 0 0 Xanthomonas nigromaculans ATCC 23390 Xrnl (Pvul) CGATCG 3 7 0 0 66 Xanthomonas oryzae M. Ehrlich Xorl (PstI) CTGCAG 18 25 2 1 188 Xorll (Pvul) CGATCiG 3 7 0 0 188 Xanthomonas papavericola ATCC 14180 Xpal (Xhol) DTCGAG 1 6 0 1 55 Downloaded from https://academic.oup.com/nar/article/9/1/213/1043263 by DeepDyve user on 31 July 2022 Footnotes to Table a. When two enzymes recognize the same sequence, i.e., are isoschizomers, the prototype (i.e., the first example isolated) is indicated in parentheses. 1 1 b . Recognition sequences are written from 5 •* 3 , only one strand being given, and the point of cleavage is indicated by an arrow (+). When no arrow appears, the precise cleavage site has not been determined. For example, GiGATCC is an abbreviation for 5 C'C A T C C 3' 3 C C T A G G 5' Bases appearing in parentheses signify that either base may occupy that position in the recognition sequence Thus , Accl cleaves the sequence GTAGAC, GTATAC, GTCGAC, and GTCTAC. Where known, the base modified by the corresponding specific methylase is indicated by an asterisk. A is N -methyladenosine. C is 5-methyl- cytosine. c. These columns indicate the frequency of cleavage by the various specific endonucleases on bacteriophage lambda DNA (K), adenovirus-2 DNA (Ad2), simian virus 40 DNA (SV40), and 0X174 Rf DNA «iX174). In the latter two cases, the sites were checked by computer search of the published sequences d. In most E. coli strains, bacteriophage lambda DNA is partially modified against the action of AtuBI, AtuII, AtuCI, Bell, BstEIII, Cgel, DpjiII, Ecall, EclII, EcoRII, FnuAII, FnuCI, Mbol, MnoIII, MosI, MphI, and Xbal. It should be noted" that FnuE I, Pfal and Sau3A are not inhibited by dam methylation; BstNI and Apyl are not inhibited by mec methylation. e. Hgal cleaves as indicated: 5 GACGCNNNNN* 3' 3' CTGCGNNNNNNNNNN 5' f. Hghl cleaves as indicated: 5' GGTGANNNNNNNN* 3 3 CCACTNNNNNNN 5' g. MboII cleaves as indicated: 5' GAAGANNNNNNNN* 3' , cTTCTNNNNNNN 5' 3 f h. Mnll cleaves 5 to 10 bases in the 3 direction from the recognition sequence. SfaNI cleaves an unknown number of nucleotides away from the recognition sequence. i. EcoPI, EcoP15, and Hinflll have characteristics intermediate between those of the Type I and Type II restriction endonucleases. They are designated Type III in accordance with the suggestion of Kauc and Piekarowicz (85). j . Tthlll II cleaves as indicated: 5' CAAPuCA (N),,i 3' 3' GTTPyGT (N) t 5' k. Hinfill cleaves about 25 bases 3' of the recognition sequence. Downloaded from https://academic.oup.com/nar/article/9/1/213/1043263 by DeepDyve user on 31 July 2022 Nucleic Acids Research REFERENCES 1. Arrand, J.R. , Myers, P.A. and Roberts, R.J. unpublished observations. 2 . Agarwal, K., unpublished observations. 3 . Arrand, J.R. , Myers, P.A. and Roberts, R.J. J. Mol. Biol. 118, 127-135 (1978). 4 . Bachi, B. , Reiser, J. and Pirrotta, V. J. Mol. Biol. 128, 143-163 (1979). 5 . Baumstark, B.R., Roberts, R.J. and RajBhandary, U.L. J. Biol. Chem. 254, 8943- 8950 (1979). 6. Bickle, T. and Ineichen, K., Gene 9, 205-212 (1980). 7. Bickle, T., Yuan, R., Pirrotta, V. and Ineichen, K., unpublished observations. 8 . Bigger, C.H., Murray, K and Murray, N.E. Nature New Biology 244, 7-10 (1973). 9 . 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Nucleic Acids Research – Oxford University Press

Published: Jan 10, 1981

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