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Taurine Down‐Regulates Basal and Osmolarity‐Induced Gene Expression of Its Transporter, but Not the Gene Expression of Its Biosynthetic Enzymes, in Astrocyte Primary Cultures

Taurine Down‐Regulates Basal and Osmolarity‐Induced Gene Expression of Its Transporter, but Not... Abstract: Taurine content of astrocytes is primarily regulated by transport from the extracellular medium and endogenous biosynthesis from cysteine. We have investigated the gene expression of the taurine transporter (TauT) and the taurine biosynthetic enzymes, cysteine dioxygenase (CDO) and cysteine sulfinate decarboxylase (CSD), in astrocyte primary cultures in relationship to cell taurine content. TauT, CDO, and CSD mRNA levels were determined through quantitative RT‐PCR. Cell taurine content was depleted by adapting the cells to a taurine‐free chemically defined medium and increased by incubating the cells in the same medium containing exogenous taurine. With increased cell taurine content the level of TauT mRNA decreased, whereas the levels of CDO and CSD mRNA remained unchanged. In astrocytes exposed to a hyperosmotic medium the TauT mRNA level increased, whereas the CDO and CSD mRNA levels were not significantly altered. The osmolarity‐induced up‐regulation of TauT mRNA expression was fully prevented by increasing cell taurine content. Thus, the gene expression of the taurine transporter, but not that of the taurine biosynthetic enzymes, appears to be under the control of two antagonistic regulations, namely, a taurine‐induced down‐regulation and an osmolarity‐induced up‐regulation. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neurochemistry Wiley

Taurine Down‐Regulates Basal and Osmolarity‐Induced Gene Expression of Its Transporter, but Not the Gene Expression of Its Biosynthetic Enzymes, in Astrocyte Primary Cultures

Bitoun, Marc; Tappaz, Marcel
Journal of Neurochemistry , Volume 75 (3) – Jan 1, 2000
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References (29)

Publisher
Wiley
Copyright
Copyright © 2000 Wiley Subscription Services
ISSN
0022-3042
eISSN
1471-4159
DOI
10.1046/j.1471-4159.2000.0750919.x
Publisher site
See Article on Publisher Site

Abstract

Abstract: Taurine content of astrocytes is primarily regulated by transport from the extracellular medium and endogenous biosynthesis from cysteine. We have investigated the gene expression of the taurine transporter (TauT) and the taurine biosynthetic enzymes, cysteine dioxygenase (CDO) and cysteine sulfinate decarboxylase (CSD), in astrocyte primary cultures in relationship to cell taurine content. TauT, CDO, and CSD mRNA levels were determined through quantitative RT‐PCR. Cell taurine content was depleted by adapting the cells to a taurine‐free chemically defined medium and increased by incubating the cells in the same medium containing exogenous taurine. With increased cell taurine content the level of TauT mRNA decreased, whereas the levels of CDO and CSD mRNA remained unchanged. In astrocytes exposed to a hyperosmotic medium the TauT mRNA level increased, whereas the CDO and CSD mRNA levels were not significantly altered. The osmolarity‐induced up‐regulation of TauT mRNA expression was fully prevented by increasing cell taurine content. Thus, the gene expression of the taurine transporter, but not that of the taurine biosynthetic enzymes, appears to be under the control of two antagonistic regulations, namely, a taurine‐induced down‐regulation and an osmolarity‐induced up‐regulation.

Journal

Journal of Neurochemistry – Wiley

Published: Jan 1, 2000

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