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Expression of Messenger RNAs Encoding Insulin-like Growth Factor-I, -II, and Insulin-like Growth Factor Binding Protein-2 in Bovine Endometrium during the Estrous Cycle and Early Pregnancy

Expression of Messenger RNAs Encoding Insulin-like Growth Factor-I, -II, and Insulin-like Growth... Abstract The present study characterized the changes in concentrations of insulin-like growth factors-I and -II (IGF-I and IGF-II) in uterine luminal flushings, and the endometrial mRNA levels of IGF-I, IGF-II, and IGF binding protein-2 (IGFBP-2) obtained from Days 0 through 18 of the bovine estrous cycle and early pregnancy. Concentrations of IGF-I and IGF-II in uterine flushings were greater on Days 0 and 5 than on other days of the estrous cycle or pregnancy. Northern blot analysis of endometrial poly(A)+ RNA revealed two major transcripts of 7.5 and 1.0 Kb for IGF-I, one major transcript of 4.0 Kb for IGF-II, and one major transcript of 1.3 Kb for IGFBP-2. RNA dot-blot analyses indicated that endometrial expression of IGF-I mRNA was unaffected by day of the estrous cycle or status (cyclic vs. pregnancy). However, endometrial expression of IGF-II mRNA was greater in pregnant than in cyclic endometrium on Days 15 and 18. Levels of endometrial IGFBP-2 mRNA increased (p < 0.05) between Days 10 and 18 of the estrous cycle and early pregnancy. Results suggest that the presence of the bovine conceptus has a stimulatory effect on endometrial expression levels of IGF-II, whereas progesterone appears to be involved with enhancement of IGFBP-2. This content is only available as a PDF. Author notes 1 This research was supported in part by USDA Grants 87-CRCR-1–2502 and 89–37265–4545: this manuscript is published as Journal Article No. 6080 of Agricultural Experiment Station, Oklahoma State University, Stillwater, Ok. copyright © 1991 by The Society for the Study of Reproduction http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biology of Reproduction Oxford University Press

Expression of Messenger RNAs Encoding Insulin-like Growth Factor-I, -II, and Insulin-like Growth Factor Binding Protein-2 in Bovine Endometrium during the Estrous Cycle and Early Pregnancy

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Publisher
Oxford University Press
Copyright
copyright © 1991 by The Society for the Study of Reproduction
ISSN
0006-3363
eISSN
1529-7268
DOI
10.1095/biolreprod45.6.975
Publisher site
See Article on Publisher Site

Abstract

Abstract The present study characterized the changes in concentrations of insulin-like growth factors-I and -II (IGF-I and IGF-II) in uterine luminal flushings, and the endometrial mRNA levels of IGF-I, IGF-II, and IGF binding protein-2 (IGFBP-2) obtained from Days 0 through 18 of the bovine estrous cycle and early pregnancy. Concentrations of IGF-I and IGF-II in uterine flushings were greater on Days 0 and 5 than on other days of the estrous cycle or pregnancy. Northern blot analysis of endometrial poly(A)+ RNA revealed two major transcripts of 7.5 and 1.0 Kb for IGF-I, one major transcript of 4.0 Kb for IGF-II, and one major transcript of 1.3 Kb for IGFBP-2. RNA dot-blot analyses indicated that endometrial expression of IGF-I mRNA was unaffected by day of the estrous cycle or status (cyclic vs. pregnancy). However, endometrial expression of IGF-II mRNA was greater in pregnant than in cyclic endometrium on Days 15 and 18. Levels of endometrial IGFBP-2 mRNA increased (p < 0.05) between Days 10 and 18 of the estrous cycle and early pregnancy. Results suggest that the presence of the bovine conceptus has a stimulatory effect on endometrial expression levels of IGF-II, whereas progesterone appears to be involved with enhancement of IGFBP-2. This content is only available as a PDF. Author notes 1 This research was supported in part by USDA Grants 87-CRCR-1–2502 and 89–37265–4545: this manuscript is published as Journal Article No. 6080 of Agricultural Experiment Station, Oklahoma State University, Stillwater, Ok. copyright © 1991 by The Society for the Study of Reproduction

Journal

Biology of Reproduction – Oxford University Press

Published: Dec 1, 1991

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