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Immunological and non‐immunological influence of H‐2K b gene transfection on the metastatic ability of B16 melanoma cells

Immunological and non‐immunological influence of H‐2K b gene transfection on the metastatic... The H‐2b‐negative B78HI clone (derived from B16 melanoma) was transfected with the H‐2Kb gene; 4 cell clones expressing membrane H‐2Kb antigens and 2 control clones (transfected with pSV2neo alone) were used for studies of metastatic ability, immunogenicity, NK sensitivity and homo‐typic adhesion. The experimental metastatic capacity of H‐2Kb transfectants In syngeneic mice was greatly diminished in comparison with control and parent cells. Both immune‐mediated and Intrinsic properties of transfectants correlated with their lower metastatic ability. A cell‐mediated cytotoxic response was induced by repeated in vivo immunizations of syngeneic mice followed by in vitro restimulation of effectors when transfectants (but not controls) were used as immunizers and as targets. Moreover, homotypic adhesion of H‐2Kbtransfectants was significantly lower than that of controls. Sensitivity to NK ceils of transfectants was not decreased in comparison to H‐2‐negative controls. It is known that In vitro treatment with IFN‐γ of H‐2‐positive B16 melanoma cells induces a simultaneous increase in H‐2 expression and in experimental metastasis; treatment of H‐2Kb transfectants with IFN‐γ induced a higher Kb expression, but no increase in metastatic ability, thus suggesting that the IFN‐sensitive component that mediates enhancement of metastasis is not H‐2Kb. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png International Journal of Cancer Wiley

Immunological and non‐immunological influence of H‐2K b gene transfection on the metastatic ability of B16 melanoma cells

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References (42)

Publisher
Wiley
Copyright
Copyright © 1991 Wiley‐Liss, Inc., A Wiley Company
ISSN
0020-7136
eISSN
1097-0215
DOI
10.1002/ijc.2910480220
Publisher site
See Article on Publisher Site

Abstract

The H‐2b‐negative B78HI clone (derived from B16 melanoma) was transfected with the H‐2Kb gene; 4 cell clones expressing membrane H‐2Kb antigens and 2 control clones (transfected with pSV2neo alone) were used for studies of metastatic ability, immunogenicity, NK sensitivity and homo‐typic adhesion. The experimental metastatic capacity of H‐2Kb transfectants In syngeneic mice was greatly diminished in comparison with control and parent cells. Both immune‐mediated and Intrinsic properties of transfectants correlated with their lower metastatic ability. A cell‐mediated cytotoxic response was induced by repeated in vivo immunizations of syngeneic mice followed by in vitro restimulation of effectors when transfectants (but not controls) were used as immunizers and as targets. Moreover, homotypic adhesion of H‐2Kbtransfectants was significantly lower than that of controls. Sensitivity to NK ceils of transfectants was not decreased in comparison to H‐2‐negative controls. It is known that In vitro treatment with IFN‐γ of H‐2‐positive B16 melanoma cells induces a simultaneous increase in H‐2 expression and in experimental metastasis; treatment of H‐2Kb transfectants with IFN‐γ induced a higher Kb expression, but no increase in metastatic ability, thus suggesting that the IFN‐sensitive component that mediates enhancement of metastasis is not H‐2Kb.

Journal

International Journal of Cancer – Wiley

Published: May 10, 1991

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