Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 7-Day Trial for You or Your Team.

Learn More →

Detection and Nucleotide Sequence Analysis of Human Caliciviruses (HuCVs) from Samples in Non‐Bacterial Gastroenteritis Outbreaks in Hokkaido, Japan

Detection and Nucleotide Sequence Analysis of Human Caliciviruses (HuCVs) from Samples in... Samples of feces and vomit collected from patients during 13 non‐bacterial gastroenteritis outbreaks which occurred in Hokkaido between 1995 and 1998 were examined by electron microscopy (EM) and reverse‐transcription polymerase chain reaction (RT‐PCR) for evidence of infection with human caliciviruses (HuCVs). In 6 food‐borne outbreaks, oysters were the probable source of infection, while the origin of HuCVs was not found out for the other 7 outbreaks. One‐hundred‐eleven of 214 stool, vomit and oyster specimens examined gave positive results by RT‐PCR, while HuCVs were detected by EM in 36 of 121 stool specimens examined. We determined the nucleotide sequences of 470‐bp or 373‐bp PCR products amplified from the RNA polymerase region of the HuCV genomes with primer sets MR3/4 and Yuri22F/R, respectively. The sequences of different strains revealed great heterogenicity, with a range of 60 to 100% homology among strains. In a few cases, a mixed genotype was found in the same patient or same outbreak by means of nested PCR and cloning of PCR products into an appropriate vector. Of the 19 different strains found, 4 strains could be classified as Norwalk virus (genogroup 1) and the other 15 strains as Snow Mountain agent (genogroup 2) based on genotyping with homology analysis. Furthermore, the strains belonging to genogroup 2 could be classified into 4 subgroups with more than 93% homology in amino acids among strains in the subgroup. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Microbiology and Immunology Wiley

Detection and Nucleotide Sequence Analysis of Human Caliciviruses (HuCVs) from Samples in Non‐Bacterial Gastroenteritis Outbreaks in Hokkaido, Japan

Download PDF
8 pages

Loading next page...
 
/lp/wiley/detection-and-nucleotide-sequence-analysis-of-human-caliciviruses-vEkOUOT30u

References (29)

Publisher
Wiley
Copyright
© owned by Center for Academic Publications Japan (Publisher)
ISSN
0385-5600
eISSN
1348-0421
DOI
10.1111/j.1348-0421.1999.tb02440.x
Publisher site
See Article on Publisher Site

Abstract

Samples of feces and vomit collected from patients during 13 non‐bacterial gastroenteritis outbreaks which occurred in Hokkaido between 1995 and 1998 were examined by electron microscopy (EM) and reverse‐transcription polymerase chain reaction (RT‐PCR) for evidence of infection with human caliciviruses (HuCVs). In 6 food‐borne outbreaks, oysters were the probable source of infection, while the origin of HuCVs was not found out for the other 7 outbreaks. One‐hundred‐eleven of 214 stool, vomit and oyster specimens examined gave positive results by RT‐PCR, while HuCVs were detected by EM in 36 of 121 stool specimens examined. We determined the nucleotide sequences of 470‐bp or 373‐bp PCR products amplified from the RNA polymerase region of the HuCV genomes with primer sets MR3/4 and Yuri22F/R, respectively. The sequences of different strains revealed great heterogenicity, with a range of 60 to 100% homology among strains. In a few cases, a mixed genotype was found in the same patient or same outbreak by means of nested PCR and cloning of PCR products into an appropriate vector. Of the 19 different strains found, 4 strains could be classified as Norwalk virus (genogroup 1) and the other 15 strains as Snow Mountain agent (genogroup 2) based on genotyping with homology analysis. Furthermore, the strains belonging to genogroup 2 could be classified into 4 subgroups with more than 93% homology in amino acids among strains in the subgroup.

Journal

Microbiology and Immunology – Wiley

Published: Jan 1, 1999

Keywords: ; ;

There are no references for this article.