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KINETICS OF NEUTRAL AMINO ACID TRANSPORT IN RAT BRAIN IN VITRO

KINETICS OF NEUTRAL AMINO ACID TRANSPORT IN RAT BRAIN IN VITRO Department of Pharmacology and Therapeutics, St. Thomas's Hospital Medical School, London, S.E.l. (Received 29 September 1966) IT IS well established that slices of brain, in common with those of other tissues, possess mechanisms for the active transport of amino acids which leads to their accumulation against a concentration gradient when the slices are incubated in a suitable medium (for references see SMITH, 1963; LAHIRI and LAJTHA, 1964). Net uptake at any time represents the difference between influx and efflux of the amino acids (NEAME SMITH, and 1965). To obtain information about the characteristics of the transport carrier or carriers involved it is necessary to separate the two processes in time or to study both together on a mathematical basis such as that used for kidney slices by ROSENBERG, BERMAN SEGAL and (1963). One limitation of the latter approach is imposed by the enormous number of observations which are needed to investigate the behaviour of the carriers towards an amino acid at more than one concentration. Another disadvantage is that it necessitates measuring steady state kinetics which may take a long incubation period to achieve in a preparation which is constantly deteriorating. The present report involves separation of the http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neurochemistry Wiley

KINETICS OF NEUTRAL AMINO ACID TRANSPORT IN RAT BRAIN IN VITRO

Smith, S. E.
Journal of Neurochemistry , Volume 14 (3) – Mar 1, 1967
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References (14)

Publisher
Wiley
Copyright
Copyright © 1967 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0022-3042
eISSN
1471-4159
DOI
10.1111/j.1471-4159.1967.tb09526.x
Publisher site
See Article on Publisher Site

Abstract

Department of Pharmacology and Therapeutics, St. Thomas's Hospital Medical School, London, S.E.l. (Received 29 September 1966) IT IS well established that slices of brain, in common with those of other tissues, possess mechanisms for the active transport of amino acids which leads to their accumulation against a concentration gradient when the slices are incubated in a suitable medium (for references see SMITH, 1963; LAHIRI and LAJTHA, 1964). Net uptake at any time represents the difference between influx and efflux of the amino acids (NEAME SMITH, and 1965). To obtain information about the characteristics of the transport carrier or carriers involved it is necessary to separate the two processes in time or to study both together on a mathematical basis such as that used for kidney slices by ROSENBERG, BERMAN SEGAL and (1963). One limitation of the latter approach is imposed by the enormous number of observations which are needed to investigate the behaviour of the carriers towards an amino acid at more than one concentration. Another disadvantage is that it necessitates measuring steady state kinetics which may take a long incubation period to achieve in a preparation which is constantly deteriorating. The present report involves separation of the

Journal

Journal of Neurochemistry – Wiley

Published: Mar 1, 1967

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