Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 7-Day Trial for You or Your Team.

Learn More →

Detection of Legionella spp. and Legionella pneumophila in water samples of Spain by specific real-time PCR

Detection of Legionella spp. and Legionella pneumophila in water samples of Spain by specific... Legionella pneumophila is the primary cause of the legionellosis diseases (90 %) (Yu et al. in J Infect Dis 186:127–128, 2002 ; Doleans et al. in J Clin Microbiol 42:458–460, 2004 ; Den Boer et al. in Clin Microbiol Infect 14:459–466, 2008 ). In this study, methodologies based on molecular biology were developed in order to provide a quick diagnosis of the bacterial presence in water samples of Spain. Multiplex real-time polymerase chain reaction assays were realized to target the 16S rRNA and macrophage infectivity potentiator ( mip) genes of, respectively, Legionella spp . and L. pneumophila including in the design of an internal control. The results obtained by the culture and the gene amplification methods agreed in 94.44 % for the 16S rRNA gene, and a concordance of 66.67 % of the cases was obtained for the mip gene. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Microbiology Springer Journals

Detection of Legionella spp. and Legionella pneumophila in water samples of Spain by specific real-time PCR

Download PDF
9 pages

Loading next page...
 
/lp/springer-journals/detection-of-legionella-spp-and-legionella-pneumophila-in-water-vf9hcnbbMw

References (6)

Publisher
Springer Journals
Copyright
Copyright © 2014 by Springer-Verlag Berlin Heidelberg
Subject
Life Sciences; Microbiology; Microbial Ecology; Biochemistry, general; Cell Biology; Biotechnology; Ecology
ISSN
0302-8933
eISSN
1432-072X
DOI
10.1007/s00203-013-0934-2
pmid
24264468
Publisher site
See Article on Publisher Site

Abstract

Legionella pneumophila is the primary cause of the legionellosis diseases (90 %) (Yu et al. in J Infect Dis 186:127–128, 2002 ; Doleans et al. in J Clin Microbiol 42:458–460, 2004 ; Den Boer et al. in Clin Microbiol Infect 14:459–466, 2008 ). In this study, methodologies based on molecular biology were developed in order to provide a quick diagnosis of the bacterial presence in water samples of Spain. Multiplex real-time polymerase chain reaction assays were realized to target the 16S rRNA and macrophage infectivity potentiator ( mip) genes of, respectively, Legionella spp . and L. pneumophila including in the design of an internal control. The results obtained by the culture and the gene amplification methods agreed in 94.44 % for the 16S rRNA gene, and a concordance of 66.67 % of the cases was obtained for the mip gene.

Journal

Archives of Microbiology – Springer Journals

Published: Jan 1, 2014

There are no references for this article.