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An improved toolbox to unravel the plant cellular machinery by tandem affinity purification of Arabidopsis protein complexes

An improved toolbox to unravel the plant cellular machinery by tandem affinity purification of... A platform for isolating low-abundance protein complexes from Arabidopsis seedlings and cell cultures is described. Its power resides in an improved TAP tag combined with ultrasensitive MS and filtering against a list of nonspecific proteins. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nature Protocols Springer Journals

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References (73)

Publisher
Springer Journals
Copyright
Copyright © 2014 by Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved.
Subject
Life Sciences; Life Sciences, general; Biological Techniques; Analytical Chemistry; Microarrays; Computational Biology/Bioinformatics; Organic Chemistry
ISSN
1754-2189
eISSN
1750-2799
DOI
10.1038/nprot.2014.199
Publisher site
See Article on Publisher Site

Abstract

A platform for isolating low-abundance protein complexes from Arabidopsis seedlings and cell cultures is described. Its power resides in an improved TAP tag combined with ultrasensitive MS and filtering against a list of nonspecific proteins.

Journal

Nature ProtocolsSpringer Journals

Published: Dec 18, 2014

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