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- Publisher
- Wiley
- Copyright
- Copyright © 2002 Wiley Subscription Services
- ISSN
- 0951-4198
- eISSN
- 1097-0231
- DOI
- 10.1002/rcm.701
- pmid
- 12112272
- Publisher site
- See Article on Publisher Site
Abstract
A new β‐elimination procedure has been introduced to cleave O‐linked oligosaccharides from low‐ to sub‐microgram amounts of glycoproteins prior to analysis by mass spectrometry. Borane‐ammonia complex in aqueous ammonia is used as a cleaving solution alternative to the sodium borohydride/sodium hydroxide medium conventionally used in β‐elimination. The procedure results in minimum sample purification, leading to minimal sample loss and consequently an overall enhancement in sensitivity. It was applied successfully in the analysis of bovine fetuin and submaxillary mucin, as well as to a complex bile‐salt‐stimulated lipase glycoprotein isolated from human milk. Copyright © 2002 John Wiley & Sons, Ltd.
Journal
Rapid Communications in Mass Spectrometry – Wiley
Published: Jan 30, 2002