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Vitamin D receptor expression in chicken muscle tissue and cultured myoblasts.

Vitamin D receptor expression in chicken muscle tissue and cultured myoblasts. Muscle has long been recognized as a target tissue for 1,25-dihydroxy-vitamin D3 (1,25[OH]2D3). Evidence of the presence of VDR is provided here, thus supporting the existence of a receptor-mediated mechanism of action of 1,25(OH)2D3. Vitamin D receptor (VDR) expression is evidenced by detection of VDR-mRNA, through reverse transcription and polymerase chain reaction (RT/PCR), in chicken muscle and muscle cells (myoblasts) as well as in a variety of tissues such as intestine, kidney, heart and brain. VDR presence is also demonstrated by Southern blot of PCR products with a specific VDR-cDNA probe and by immunocytochemistry carried out on myoblasts and cardiac myocytes. Localization of VDR is mainly nuclear and more faintly detected in the cytosol. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme Pubmed

Vitamin D receptor expression in chicken muscle tissue and cultured myoblasts.

Zanello, S B; Collins, E D; Marinissen, M J; Norman, A W; Boland, R L
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme , Volume 29 (5): -224 – Sep 5, 1997
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Vitamin D receptor expression in chicken muscle tissue and cultured myoblasts.


Abstract

Muscle has long been recognized as a target tissue for 1,25-dihydroxy-vitamin D3 (1,25[OH]2D3). Evidence of the presence of VDR is provided here, thus supporting the existence of a receptor-mediated mechanism of action of 1,25(OH)2D3. Vitamin D receptor (VDR) expression is evidenced by detection of VDR-mRNA, through reverse transcription and polymerase chain reaction (RT/PCR), in chicken muscle and muscle cells (myoblasts) as well as in a variety of tissues such as intestine, kidney, heart and brain. VDR presence is also demonstrated by Southern blot of PCR products with a specific VDR-cDNA probe and by immunocytochemistry carried out on myoblasts and cardiac myocytes. Localization of VDR is mainly nuclear and more faintly detected in the cytosol.

 
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ISSN
0018-5043
DOI
10.1055/s-2007-979027
pmid
9228208

Abstract

Muscle has long been recognized as a target tissue for 1,25-dihydroxy-vitamin D3 (1,25[OH]2D3). Evidence of the presence of VDR is provided here, thus supporting the existence of a receptor-mediated mechanism of action of 1,25(OH)2D3. Vitamin D receptor (VDR) expression is evidenced by detection of VDR-mRNA, through reverse transcription and polymerase chain reaction (RT/PCR), in chicken muscle and muscle cells (myoblasts) as well as in a variety of tissues such as intestine, kidney, heart and brain. VDR presence is also demonstrated by Southern blot of PCR products with a specific VDR-cDNA probe and by immunocytochemistry carried out on myoblasts and cardiac myocytes. Localization of VDR is mainly nuclear and more faintly detected in the cytosol.

Journal

Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme – Pubmed

Published: Sep 5, 1997

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